Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (4): 794-800.doi: 10.3864/j.issn.0578-1752.2012.04.021

• VETERINARY SCIENCE • Previous Articles     Next Articles

Expression, Sequence Analysis and Immunohistochemical Localization of Mitf-M Transcription Factor in Alpaca Skin

 ZHU  Zhi-Wei, HE  Jun-Ping, YU  Xiu-Ju, CHENG  Zhi-Xue, DONG  Chang-Sheng   

  1. 1.山西农业大学生命科学学院，山西太谷 030801
    2.山西农业大学动物科技学院，山西太谷 030801
  • Received:2011-09-12 Online:2012-02-15 Published:2011-12-08

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Abstract: 【Objective】 Cloning, sequence analysis and tissue location of the Mitf-M CDS domain sequence of alpaca skin could provide a foundation for studying coat color expression mechanism.【Method】The CDS domain sequence of Mitf-M was amplified by RT-PCR, multiple sequence alignment of the amino acid sequence was analyzed by Clustal X and BioEdit software. The distribution of Mitf-M protein in alpaca skin was studied by immunohistochemistry.【Result】Mitf-M CDS domain of alpaca is composed by 419 amino acids, with bHLH-zip conserved domain of transcription factor family. Phylogenetic analysis showed that Mitf-M had the highest homology with cattle (Bos Taurus) and dogs (Canis familiaris). The Mitf-M protein was mainly located in melancytes during the basal cells of hair bulb and around dermis papilla. 【Conclusion】 Compared with other species, Mitf-M in alpaca skin are highly conservative, indicating that Mitf-M plays an important role in the transcriptional regulation and pigmentation.

Key words: alpaca (Lama pacos), skin, hair follicles, Mitf-M, PCR, immunohistochemistry

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