Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (2): 367-375.doi: 10.3864/j.issn.0578-1752.2013.02.016

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Cloning and Testicular Expression Level of b-Sycp2 in Cattle, Yak and Cattle-Yak

 LUO  Hua, JIA  Chao, QU  Xu-Guang, ZHAO  Xing-Bo, ZHONG  Jin-Cheng, XIE  Zhuang, LI  Qi-Fa   

  1. 1.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095
    2.College of Animal Science and Technology, China Agricultural University, Beijing 100193
    3.College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041
  • Received:2012-05-11 Online:2013-01-15 Published:2012-07-03

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Abstract: 【Objective】The study was aimed to clone the Sycp2 gene sequence of cattle, yak and cattle-yak, investigate the gene structures and its tissue-expression patterns and testicular expression levels in cattle, yak and cattle-yak.【Method】 In silico cloning and clone sequencing were applied to acquire the coding region sequences of Sycp2 gene of cattle, yak and yak-cattle, and molecular characterization were analyzed by bioinformatics software. RT-PCR was applied to analyze the tissue-expression patterns, and real-time PCR was employed to examine the expression levels in cattle, yak and cattle-yak testis.【Result】The full length of the coding region sequences of the Sycp2 gene was 4 365 bp, named b-Sycp2. The b-Sycp2 gene in cattle, yak and cattle-yak encoded 1 454 amino acid residues which included some typical domains such as coiled-coil domain. RT-PCR analysis showed that b-Sycp2 gene was expressed only in the testis. Real-time PCR analysis revealed that the mRNA expression level of b-Sycp2 gene in the testis of cattle and yak was remarkably higher than that of in cattle-yak (P＜0.05). 【Conclusion】The b-Sycp2 gene was cloned successfully, and it expressed only in testis, b-Sycp2 mRNA expression level of cattle and yak represented a dramatic higher than that of cattle-yak.

Key words: cattle , yak , cattle-yak , b-Sycp2 gene , clone , expression level

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