Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (1): 179-186.doi: 10.3864/j.issn.0578-1752.2013.01.021

• VETERINARY SCIENCE • Previous Articles     Next Articles

Construction of a Chimeric Classical Swine Fever Virus C-strain Containing E2 of Group Ⅱ Isolate Remains and Its Biological Characteristics

 TONG  Chao, CHEN  Ning, LIAO  Xun, YUAN  Xue-Mei, LI  Xiao-Liang, FANG  Wei-Huan   

  1. Institute of Preventive Veterinary Medicine, Zhejiang University/Laboratory of Prevent Veterinary Medicine, Hangzhou 310058
  • Received:2012-11-15 Online:2013-01-01 Published:2012-12-09

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Abstract: 【Objective】 The classical swine fever (CSF) C-strain vaccine has proven to be safe and highly efficacious. However, classical swine fever virus (CSFV) genotype Ⅱisolates became dominant in recent years, making it more challenging to bring the disease into total control. This study was attempted to engineer, by reverse genetic approach, a novel C-strain-based vaccine candidate that matches molecular variations of field isolates, and to characterize its biological features. Those results could provide foundations for further development of a marker vaccine for differential diagnostic purpose.【Method】An infectious recombinant CSF virus was generated by exchanging the 5’-end 870-bp of E2 gene in C-strain with the same fragment from group 2 strain HZ1-08. The resulting recombinant virus RecCHZE2 was rescued and its pathogenicity and immunogenicity were characterized in rabbits and pigs. 【Result】 Rabbits inoculated with RecCHZE2 exhibited typical temperature profiles similar to the C-strain with spleen enlargement. Pigs inoculated with the recombinant chimeric virus did not show fever and significant decline of the number of white blood cells. Mild transient viremia was detected at day 12 post-inoculation. The antibody responses specific to the field strain HZ1-08 reached the peak 20 days post-inoculation. The neutralizing antibody titers from pigs immunized with RecCHZE2 were similar to both HZ1-08 and C strains while the titer from pigs immunized with RecC were lower for group 2 strain HZ1-08.【Conclusion】The recombinant virus RecCHZE2 remains avirulent but differs in immunogenic properties from the C-strain.

Key words: classical swine fever virus , chimeric C-strain virus , pathogenicity , immunogenicity

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