Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (23): 4931-4938.doi: 10.3864/j.issn.0578-1752.2012.23.021

• RESEARCH NOTES • Previous Articles     Next Articles

Molecular Maker Screen for High Oleic Acid in Space Flight Mutant Brassica napus

 LIU  Lie-Zhao, WANG  Xin-Na, YAN  Xing-Ying, WANG  Rui, XU  Xin-Fu, LU  Kun, LI  Jia-Na   

  1. College of Agronomy and Biotechnology, Southwest University/Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400716
  • Received:2012-08-09 Online:2012-12-01 Published:2012-10-12

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Abstract: 【Objective】 A study was conducted to analyze the mutant locus and screen SSR markers tightly linked to high oleic acid in the high oleic acid space flight mutant derived F2 population by using the SSR markers beside FAD2 gene. 【Method】 The F2 population was derived from the cross of yellow seeded low oleic acid/high linolenic acid self line 10L421 and space flight mutant high oleic acid/ low linolenic acid self line 10L422. Totally, 148 SSR markers beside FAD2 genes by 100 kb region in both sides from chromosomes A05, A01, C05 and C01 were analyzed in the two parents, high and low oleic acid bulks and F2 population. The parents and F2 plants oleic acid were analyzed by GC. Marker and oleic acid relationship were analyzed by single marker test.【Result】There were 36 of the SSR markers were polymorphic among the two parents, ten SSR markers showed a cosegregation in the high and low oleic acid extreme plants. The SSR markers beside FAD2 gene in A05 and A01 were significantly related to the oleic acid and accounted for 31.1% and 29.4% of the oleic acid variation by single marker analysis. 【Conclusion】 The ANOVA and single marker analysis indicated that the FAD2 genes in A05 and A01 are the major loci responsible for the oleic acid variation in this space flight mutant derived population, which means that the double recessive mutation of FAD2 genes in A05 and A01 lead to the high oleic acid content of the space flight mutant.

Key words: Brassica napus, molecular marker, oleic acid, FAD2

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