Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (20): 4130-4140.doi: 10.3864/j.issn.0578-1752.2012.20.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Study on Microspore Abortion of Male Sterile Cotton Yamian A and Yamian B

 ZHAO  Hai-Yan, HUANG  Jin-Ling   

  1. 1.山西农业大学农学院，山西太谷 030801）
  • Received:2012-06-20 Online:2012-10-15 Published:2012-08-16

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Abstract: 【Objective】 Cell morphological observation and comparison of physiology and biochemistry characteristics of the new male sterile materials Yamian A and its maintainer Yamian B in cotton were studied.【Method】 Yamian A was identified as a new and stable cytoplasmic male sterile line derived from the triple hybrids of G. arboreum, G. bickii and G. herbaceum. This study involved observation of cell morphology and comparison of physiology and biochemicaI characteristics of the new male sterile materials Yamian A and its maintainer Yamian B by means of optical and electron microscopy and measurement of physiology and biochemical indexes.【Result】 Microspore abortion of Yamian A occurred mainly between the stages of sporogenous cell and leptotene. In the microscopic structure, the indications of abortion were that the sporogenous cells were adhesive, the sporogenous cells were without nuclei, then some of them were dissolved at the sporogenous cell stage, the sporogenous cells developed into microsporocyte without going through disintegration, then the nucleus disappeared, and completely disintegrated before the leptotene stage. Compared with the fertile line, the tapetal cell development in the sterile line were delayed, both the tapetal cells and middle layer cells did not collapse in the whole process of anther development. All these abnormal phenomenon resulted in the formation of pollen sacs without pollen grains finally. In the submicroscopic structure, the corresponding tapetal cells also showed an abnormal phenomenon of mitochondrial degradation, while the sporogenous cells and microsporocyte showed degradation signs of mitochondrial cristae, endoplasmic reticulum fracture, and so on. The peroxidase activities in the abortion flower buds of Yamian A were significantly higher than that in its maintainer Yamian B, but its succinodehydrogenase activities were lower than that in its maintainer Yamian B, the superoxide dismutase and cytochrome oxidase activities in the leaves and flower buds of different development stages were both lower than that in its maintainer Yamian B.【Conclusion】 The main cause leading to male sterility of Yamian A may be the delayed development of the tapetal cells, and the changes in the activities of peroxidase, superoxide dismutase, cytochrome oxidase and succinodehydrogenase.

Key words: cotton, cytoplasmic male sterility, microspore, cytology, physiology and biochemistry

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