高渗胁迫对玉米大斑病菌生长发育及STK1表达的影响

doi:10.3864/j.issn.0578-1752.2012.19.007

Abstract

Abstract: 【Objective】The objective of this study is to measure the effect of hyperosmotic stress on the growth and development of Setosphaeria turcica, to analyze whether or not the glycerol is one of the compatible solutes, and to detect the expression characteristic of STK1 when the pathogen is cultured under hyperosmotic stress. 【Method】 The growth and development of S. turcica, which cultured under 2 different hyperosmotic stresses, were observed. By analyzing the change of glycerol content in mycelium cells, it was confirmed that glycerol was one of the compatible solutes. The expression characteristic of STK1 was detected using semiquantitative RT-PCR method.【Result】Isotonic solution concentration of hyphal cells of S. turcica was 0.78 mol•L-1. The colony color changed distinctly under hyperosmotic stress. The colony cultured on PDA medium containing 1 mol•L-1 NaCl was reddish-brown in colour, the growth rate of the colony was repressed remarkably, cultured on PDA medium containing 1 mol•L-1 NaCl showed strong inhibition. The condensation was observed in the protoplasts when cultured in 1 mol•L-1 NaCl treatment. With the increase of hyperosmotic stress time and compatible solute concentration, glycerol content increased evidently. The expression level of STK1 enhanced distinctly within 48 hours under hyperosmotic stress.【Conclusion】Colony growth rate was inhibited, colony color turned obviously, protoplast became concentrated, some mycelium cells enlarged and changed into globosed under hyperosmotic stress in S. turcica. Glycerol was one of the compatible solutes in the mycelium cells of S. turcica. STK1 took part in regulating hyperosmotic stress responses in S. turcica.

Key words: Setosphaeria turcica, hyperosmotic stress, glycerol, STK1

WANG Mei-Juan, LI Po, WU Min, FAN Yong-Shan, GU Shou-Qin, DONG Jin-Gao. Effect of Hyperosmotic Stress on the Growth, Development and STK1 Expression of Setosphaeria turcica[J].Scientia Agricultura Sinica, 2012, 45(19): 3965-3970.

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Effect of Hyperosmotic Stress on the Growth, Development and STK1 Expression of Setosphaeria turcica

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