玉米大斑病菌黑色素合成酶基因的全基因组定位及表达模式分析

doi:10.3864/j.issn.0578-1752.2015.14.008

Abstract

Abstract: 【Objective】The objectives of this study are to determine the location in the genome, and to investigate the structure of melanin biosynthesis genes StPKS, StSCD, St3HNR, St4HNR, StLAC1 and StLAC2 of Setosphaeria turcica. Furthermore, to elucidate the relationship between melanin biosynthesis enzyme genes and morphogenesis and pathogenicity of S. turcica viaanalyzing the expression pattern of six genes during infection progress from conidial germination to penetration and the mycelia growth period.【Method】 Based on the S. turcica genome database and genome searching with Blastp, the locations of six genes were identified and genes linkage were analyzed. Primers were designed according to the six melanin synthesis enzyme gene sequences. qRT-PCR system was used to detect the six genes expressions of S. turcica, in which total RNAs from different infectious morphogenesis stages including conidial germination to penetration were extracted and used as templates and β-tubulin as the reference gene, and the expression patterns between vegetative and reproductive growth stages were compared.【Result】In genome database, the StPKS and St3HNR were located on the positive and negative strand of scaffold_12, respectively. St4HNR and StLAC2 were on the negative strand scaffold_11. StSCD and StLAC1 located on the positive of strand scaffold_1 and scaffold_7, respectively. Furthermore, StPKS and St3HNR were clustered within the 26.9 kb chromosomal region. Relative expression of six genes had two expression profiles, which was up-down- up regulation or up- down- up- down regulation at five stages from conidial germination to penetration. The StLAC2 had the highest expression in conidia, and there was a significant difference in expression. However, St3HNR and StSCD had a higher expression level than other genes in mycelia.【Conclusion】StPKS and St3HNR in melanin biosynthesis were clustered within a 26.9 kb chromosomal region. Six genes were whole expressed at five stages from conidial germination to penetration. They had a similar expression pattern. Unfortunately, they had a significant difference in expression levels. The results showed that six genes of melanin biosynthesis enzyme participated in the infection process and played an important role in the pathogenic of S. turcica. In addition, St3HNR and StSCD played an important role at mycelial stage. StLAC2 had the highest expression at conidial stage.

Key words: Setosphaeria turcica; melanin, biosynthesis enzyme gene, location, expression pattern

JIA Hui, MENG Qing-jiang, LI Zhi-yong, GONG Xiao-dong, ZANG Jin-ping, HAO Zhi-min, CAO Zhi-yan, DONG Jin-gao. Localization of Melanin Biosynthesis Enzyme Genes in the Genome and Expression Pattern Analysis of Setosphaeria turcica[J].Scientia Agricultura Sinica, 2015, 48(14): 2767-2776.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2015.14.008

https://www.chinaagrisci.com/EN/Y2015/V48/I14/2767

References

[1] 郭丽媛, 贾慧, 曹志艳, 谷守芹, 孙淑琴, 董金皋. 玉米大斑病菌有性杂交后代的交配型与寄生适合度分化. 中国农业科学, 2013, 46(19): 4058-4065.

Guo L Y, Jia H, Cao Z Y, Gu S Q, Sun S Q, Dong J G. Analysis on mating type and parasitic fitness diversity in sexual hybridization offsprings of Setosphaeria turcica. Scientia Agricultura Sinica, 2013, 46(19): 4058-4065. (in Chinese)

[2] Dong J G, Fan Y S, Gui X M, An X L, Ma J F, Dong Z P. Geographic distribution and genetic analysis of physiological races of Setosphaeria turcica in Northern China. American Journal of Agricultural and Biological Sciences, 2008, 3(1): 389-398.

[3] Tsuji G, Tsuge S, Shiraishi T, Kubo Y. Expression pattern of melanin biosynthesis enzymes during infectious morphogenesis of Colletotrichum lagenarium. Journal of General Plant Pathology, 2003, 69: 169-175.

[4] 范永山, 曹志艳, 谷守芹, 董金皋. 不同诱导因素对玉米大斑病菌附着胞产生的影响. 中国农业科学, 2004, 37(5): 769-772.

Fan Y S, Cao Z Y, Gu S Q, Dong J G. Effect of different induction factors on appressorium of Setosphaeria turcica. Scientia Agricultura Sinica, 2004, 37(5): 769-772. (in Chinese)

[5] 曹志艳, 董金皋, 杨胜勇, 姚晓霞. 玉米大斑病菌黑色素的一些理化性质和光谱吸收特征. 植物病理学报, 2007, 37(4): 410-417.

Cao Z Y, Dong J G, Yang S Y, Yao X X. Characteristics and spectral analysis of melanin in Setosphaeria turcica. Acta Phytopathologica Sinica,2007, 37(4): 410-417. (in Chinese)

[6] 吴楠, 李青为, 曹志艳, 张娇, 郝志敏, 董金皋. 玉米大斑病菌的胞外黑色素种类及影响其产量的因素. 中国农业科学, 2013, 46(5): 927-933.

Wu N, Li Q W, Cao Z Y, Zhang J, Hao Z M, Dong J G. Determination and characterization of extracellular melanin from Setosphaeria turcica and influencing factors of its production. Scientia Agricultura Sinica, 2013, 46(5): 927-933. (in Chinese)

[7] Jackson J C, Higgins L A, Lin X. Conidiation color mutants of Aspergillus fumigatus are highly pathogenic to the heterologous insect host Galleria mellonella. PLoS ONE, 2009, 4(1): e4224.

[8] Lanisnik R T, Wheeler M H. Melanin biosynthesis in the fungus Curvularia lunata (teleomorph: Cochliobolus lunatus). Canadian Journal of Microbiology, 2003, 49(2): 110-119.

[9] Keller N P, Hohn T M. Metabolic pathway gene clusters in filamentous fungi. Fungal Genetics and Biology, 1997, 21: 17-29.

[10] Kim J E, Kim J C, Jin J M, Yun S H, Lee Y W. Functional characterization of genes located at the aurofusarin biosynthesis gene cluster in Gibberella zeae. Plant Pathology Journal, 2008, 24(1): 8-16.

[11] Loppnau P, Tanguay P, Breuil C. Isolation and disruption of the melanin pathway polyketide synthase gene of the softwood deep strain fungus Ceratocystis resinifera. Fungal Genetics and Biology, 2004, 41(1): 33-41.

[12] Xue C S, Wu D L, Condon B J, Bi Q, Wang W W, Turgeon G. Efficient gene knockout in the maize pathogen Setosphaeria turcica using Agrobacterium tumefaciens-mediated transformation. Phytopathology, 2013, 103(6): 641-647.

[13] Zhang C Q, Zhu G N, Ma Z H, Zhou M G. Isolation, Characterization and preliminary genetic analysis of laboratory tricyclazole-resistant mutants of the rice blast fungus, Magnaporthe grisea. Journal of Phytopathology, 2006, 154(78): 392-397.

[14] Saitoh Y, Izumitsu K, Morita A, Shimizu K, Tanaka C. Cloning of Sal1, a scytalone dehydratase gene involved in melanin biosynthesis in Cochliobolus heterostrophus. Mycoscience,2012, 53: 330-334.

[15] Pukkila-Worley R, Gerrald Q D, Kraus P R, Boily M J, Davis M J, Giles S S, Cox G M, Heitman J, Alspaugh J A. Transcriptional network of multiple capsule and melanin genes governed by the Cryptococcus neoformans cyclic AMP cascade. Eukaryotic Cell, 2005, 4(1): 190-201.

[16] Tsai H F, Wheeler M H, Chang Y C, Kwon-Chung K J. A developmentally regulated gene cluster involved in conidial pigment biosynthesis in Aspergillus fumigatus. Journal of Bacteriology, 1999, 181(20): 6469-6477.

[17] Eliahu N, Igbaria A, Rose M S, Horwitz B A, Lev S. Melanin biosynthesis in the maize pathogen Cochliobolus heterostrophus depends on two mitogen-activated protein Kinases, Chk1 and Mps1, and the transcription factor Cmr1. Eukaryotic Cell, 2007, 6(3): 421-429.

[18] Duressa D, Anchieta A, Chen D Q, Klimes A, Garcia-Pedrajas M D, Dobinson K F , Klosterman S J. RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahlia. BMC Genomics,2013, 14: 607.

[19] Kimura N, Tsuge T. Gene cluster involved in melanin biosynthesis of the filamentous fungus Alternaria alternata. Journal of Bacteriology, 1993, 175(14): 4427-4435.

[20] Deising H B, Werner S, Wernitz M. The role of fungal appressoria in plant infection. Microbes and Infection, 2000, 2(13): 1631-1641.

[21] Butler M J, Day A W, Henson J M, Money N P. Pathogenic properties of fungal melanins. Mycologia, 2001, 93(1): 1-8.

[22] Ludwig N, Löhrer M, Hempel M, Mathea S, Schliebner I, Menzel M, Kiesow A, Schaffrath U, Deising H B, Horbach R. Melanin is not required for turgor generation but enhances cell-wall rigidity in appressoria of the corn pathogen Colletotrichum graminicola. Molecular Plant-Microbe Interactions, 2014, 27(4): 315-327.

[23] Saitoh Y, Izumitsu K, Tanaka C. Phylogenetic analysis of heavy-metal ATPases in fungi and characterization of the copper-transporting ATPase of Cochliobolus heterostrophus. Mycological research, 2009, 113: 737-745.

[24] Funa N, Funabashi M, Ohnishi Y, Horinouchi S. Biosynthesis of hexahydroxyperylenequinone melanin via oxidative Aryl coupling by cytochrome P-450 in Streptomyces griseus. Journal of Bacteriology, 2005, 187(23): 8149-8155.

[25] 张鑫, 曹志艳, 刘士伟, 郭丽媛, 董金皋. 玉米大斑病菌聚酮体合成酶基因StPKS 功能分析. 中国农业科学, 2011, 44(8): 1603-1609.

Zhang X, Cao Z Y, Liu S W, Guo L Y, Dong J G. Functional analysis of polyketide synthase gene StPKS in Setosphaeria turcica. Scientia Agricultura Sinica, 2011, 44(8): 1603-1609. (in Chinese)

[26] 曹志艳, 贾慧, 朱显明, 董金皋. DHN黑色素与玉米大斑病菌附着胞膨压形成的关系. 中国农业科学, 2011, 44(5): 925-932.

Cao Z Y, Jia H, Zhu X M, Dong J G. Relationship between DHN melanin and formation of appressorium turgor pressure of Setosphaeria turcica. Scientia Agricultura Sinica, 2011, 44(5): 925-932. (in Chinese)

[27] 于清, 曹志艳, 董金皋. 玉米大斑病菌小柱孢酮脱水酶基因(scd)的克隆与功能分析. 微生物学报, 2007, 47(6): 1013-1018.

Yu Q, Cao Z Y, Dong J G. Cloning and functional analysis of scytalone dehytratase gene in Exserohilum turcicum. Acta Microbiologica Sinica, 2007, 47(6): 1013-1018. (in Chinese)

[28] Saitoh Y, Izumitsu K, Morita A, Shimizu K, Tanaka C. ChMCO1 of Cochliobolus heterostrophus is a new class of metallo-oxidase, playing an important role in DHN-melanization. Mycoscience, 2010, 51: 327-336.

Related Articles 15

[1]	ZHANG MingJing,HAN Xiao,HU Xue,ZANG Qian,XU Ke,JIANG Min,ZHUANG HengYang,HUANG LiFen. Effects of Elevated Temperature on Rice Yield and Assimilate Translocation Under Different Planting Patterns [J]. Scientia Agricultura Sinica, 2021, 54(7): 1537-1552.
[2]	Qian CAI,ZhanXiang SUN,JiaMing ZHENG,WenBin WANG,Wei BAI,LiangShan FENG,Ning YANG,WuYan XIANG,Zhe ZHANG,Chen FENG. Dry Matter Accumulation, Allocation, Yield and Productivity of Maize- Soybean Intercropping Systems in the Semi-Arid Region of Western Liaoning Province [J]. Scientia Agricultura Sinica, 2021, 54(5): 909-920.
[3]	TAO YouFeng,PU ShiLin,ZHOU Wei,DENG Fei,ZHONG XiaoYuan,QIN Qin,REN WanJun. Canopy Population Quality Characteristics of Mechanical Transplanting Hybrid Indica Rice with “Reducing Hills and Stabilizing Basic-Seedlings” in Low-Light Region of Southwest China [J]. Scientia Agricultura Sinica, 2021, 54(23): 4969-4983.
[4]	TONG Jin,SUN Min,REN AiXia,LIN Wen,YU ShaoBo,WANG Qiang,FENG Yu,REN Jie,GAO ZhiQiang. Relationship Between Plant Dry Matter Accumulation, Translocation, Soil Water Consumption and Yield of High-Yielding Wheat Cultivars [J]. Scientia Agricultura Sinica, 2020, 53(17): 3467-3478.
[5]	SUN Hong,JIANG YiWen,YU Xin,XIANG GuangQing,YAO YuXin. Effects of Local Root Zone Salinity on Grapevine Injury, Na ⁺ Accumulation and Allocation of Carbon and Nitrogen [J]. Scientia Agricultura Sinica, 2019, 52(7): 1173-1182.
[6]	LI YongHu,CAO MengLin,DU HuiLing,GUO PingYi,ZHANG HaiYing,GUO MeiJun,YUAN XiangYang. Effect of Fertilization Location and Amount on Dry Matter Accumulation, Translocation and Yield of Hybrid Millet [J]. Scientia Agricultura Sinica, 2019, 52(22): 4177-4190.
[7]	YunPeng HOU,LiLi KONG,HongGuang CAI,HuiTao LIU,YuShan GAO,YongJun WANG,LiChun WANG. The Accumulation and Distribution Characteristics on Dry Matter and Nutrients of High-Yielding Maize Under Drip Irrigation and Fertilization Conditions in Semi-Arid Region of Northeastern China [J]. Scientia Agricultura Sinica, 2019, 52(20): 3559-3572.
[8]	LI Ting, LI ShiQing, ZHAN Ai, LIU JianLiang. Effects of Film Mulching, Nitrogen Fertilizer, Plant Density and Its Interaction on Nitrogen Accumulation, Translocation and Production Efficiency of Spring Maize on Dryland of Loess Plateau [J]. Scientia Agricultura Sinica, 2018, 51(8): 1504-1517.
[9]	MING Yi,ZHANG XiZhou,YU HaiYing. The Evaluation of Cd Accumulation in Grains of Different Wheat Materials [J]. Scientia Agricultura Sinica, 2018, 51(22): 4219-4229.
[10]	WANG DaGang, LI Kai, ZHI HaiJian. Progresses of Resistance on Soybean Mosaic Virus in Soybean [J]. Scientia Agricultura Sinica, 2018, 51(16): 3040-3059.
[11]	ZHANG WenYing, WANG Chen, ZHU XuDong, MA Chao, WANG WenRan, LENG XiangPeng, ZHENG Ting, FANG JingGui. Genome-Wide Identification and Expression of DELLA Protein Gene Family During the Development of Grape Berry Induced by Exogenous GA [J]. Scientia Agricultura Sinica, 2018, 51(16): 3130-3146.
[12]	YAN Li, WANG CuiPing, CHEN JianWei, QIAO GaiXia, LI Jian. Analysis of MYB Transcription Factor Family Based on Transcriptome Sequencing in Lycium ruthenicum Murr. [J]. Scientia Agricultura Sinica, 2017, 50(20): 3991-4002.
[13]	NIU XiaoLi, HU TianTian, ZHANG FuCang, DUAN AiWang, LIU ZhanDong, SHEN XiaoJun . Effect of Partial Water Resupply at Seedling Stage on Maize Growth, Water Absorption Capacity and Anatomical Structure [J]. Scientia Agricultura Sinica, 2017, 50(16): 3110-3121.
[14]	LIU Yi-ke, TONG Han-wen, ZHU Zhan-wang, CHEN Ling, ZOU Juan, ZHANG Yu-qing, GAO Chun-bao. Progress in Research on Mechanism of Resistance to Fusarium Head Blight in Wheat [J]. Scientia Agricultura Sinica, 2016, 49(8): 1476-1488.
[15]	XU Bei-bei, YOU Cui-cui, DING Yan-feng, WANG Shao-hua. Effect of Source-Sink Manipulation on Translocation of Carbohydrate and Nitrogen, Phosphors, Potassium in Vegetative Organs of Conventional Japonica Rice After Anthesis [J]. Scientia Agricultura Sinica, 2016, 49(4): 643-656.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!

Localization of Melanin Biosynthesis Enzyme Genes in the Genome and Expression Pattern Analysis of Setosphaeria turcica

RichHTML

PDF