玉米大斑病菌MAPK超家族的全基因组鉴定及途径模型建立

doi:10.3864/j.issn.0578-1752.2014.09.006

Abstract

Abstract: 【Objective】 Identification of MAPK superfamily genes from wide genome, and analysis of phylogeny, gene structure, multiple sequence alignment and conservative sites in Setosphaeria turcica will be useful for further research on the functions of MAPK cascade pathway in plant pathogenic fungus.【Method】Based on S. turcica genome database and genome searching with HMMER 3.0, MAPK superfamily genes were identified and their gene sequences, and genome location were obtained. The conservative protein kinase domains and sites were analyzed with ClustalX, MEME tools.【Result】Four MAPK genes, three MAPKK genes and three MAPKKK genes in S. turcica genome were found, and the MAPK was classified into Kss1/Fus3, Slt2, Hog1 and Ime2 four classes, MAPKK was classified into Pbs2, Ste7 and Mkk1 three classes, MAPKKK was classified into Ste11, Bck1 and Ssk2 three classes. Genome location and gene structure analysis showed that MAPK superfamily genes extensively distributed in the genome, and the structure of MAPK genes was the most complicated, MAPKK taken the second place, and MAPKKK structure was the simplest. Multiple sequence alignment and conservative sites analysis showed that the MAPK superfamily of kinase domain structure was highly conserved in S. turcica, and MAPK contained phosphorylation sites (-TxY-), MAPKK contained conservative phosphorylation sites (-SD[V/I]WS-), MAPKKK contained specificity conservative sites (-G[S/T] [V/P][F/M][W/Y]M[A/S]PEV-).【Conclusion】Genome-wide analysis showed that there were four MAPK genes, three MAPKK genes and three MAPKKK genes in S. turcica. Phylogenetic, gene structure, multiple sequence alignment and conservative site analysis showed that there were Fus3/Kss1-homolog, Slt2-homolog, Hog1-homolog and Ime2-homolog four MAPK cascade pathways, and Ime2 homolog may be a newly discovered MAPK cascade pathway in S. turcica. These results are helpful for the further functional research of MAPK superfamily in plant pathogenic fungus.

Key words: Setosphaeria turcica , MAPK superfamily , phylogenetic analysis , conservative motif

GONG Xiao-Dong-1, ZHANG Xiao-Yu-1, TIAN Lan-1, WANG Xing-Yi-1, LI Po-2, ZHANG Pan-1, WANG Yue-1, FAN Yong-Shan-3, HAN Jian-Min-1, GU Shou-Qin-1, DONG Jin-Gao-1. Genome-Wide Identification MAPK Superfamily and Establishment of the Model of MAPK Cascade Pathway in Setosphaeria turcica[J].Scientia Agricultura Sinica, 2014, 47(9): 1715-1724.

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Genome-Wide Identification MAPK Superfamily and Establishment of the Model of MAPK Cascade Pathway in Setosphaeria turcica

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