Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (18): 3773-3781.doi: 10.3864/j.issn.0578-1752.2012.18.010

• SOIL & FERTILIZER·WATER-SAVING IRRIGATION·AGROECOLOGY & ENVIRONMENT • Previous Articles     Next Articles

Comparison of Soil Organic Carbon, Microbial Diversity and Enzyme Activity of Wetlands and Rice Paddies in Jingjiang Area of Hubei, China

 JIN  Zhen-Jiang, TAI  Ji-Cheng, PAN  Gen-Xing, LI  Lian-Qing, SONG  Xiang-Yun, XIE  Tian, LIU  Xiao-Yu, WANG  Dan   

  1. 1.南京农业大学农业资源与生态环境研究所，南京 210095
    2.桂林理工大学环境科学与工程学院，广西桂林541004
    3.内蒙古民族大学农学院，内蒙古通辽028042
  • Received:2012-01-18 Online:2012-09-15 Published:2012-05-29

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Abstract: 【Objective】The purpose of this study is to, by means of a comparative analysis of soil organic carbon (SOC), microbial community abundance and soil enzyme activity, look into the relationship between SOC and microbial abundance/activity after rice cultivation of wetland soil for several decades. 【Method】 The topsoil (0-20 cm) samples of uncultivated wetland and paddy field were collected in Jingjiang area, Hubei, China. Contents of SOC, soil microbial biomass carbon (SMB-C) and microbial community abundance were analyzed using wet digestion with potassium dichromate, chloroform fumigation-extraction, dilute plate incubation counting and PCR-DGGE assays respectively. Microbial activity was examed using soil enzyme assays as well. 【Result】 The results showed that the SOC content increased by 55.42%, in accordance to the increases of total N and alkali-hydrolyzable N in rice paddies compared to wetland. SMB-C was found 180% higher in rice paddies than in wetlands. There was no significant difference in abundance of a single microbial community of bacterial, fungi, actionmycetes and autotrophic azotobacter, neither in diversity of bacterial and fungi between wetlands and rice paddies. However, microbial enzyme activities of invertase, urease and alkaline phosphatase was enhanced by 89%, 70% and 72%, respectively, in rice paddy over wetlands. Statistical analysis revealed a significant correlation of SMB-C and normalized overall enzyme activity index with soil organic carbon contents, respectively. 【Conclusion】 These results indicate that the size of soil microbial biomass and microbial functional activity with enzymes were enhanced as SOC storage increased due to rice cultivation of wetlands for several decades. Therefore, SMB-C size and normalized enzyme activity could be used as indicators for soil functioning by SOC.

Key words: wetland, rice paddy, soil organic carbon, microbial diversity, DGGE, soil enzyme, soil functioning

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