Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (16): 3231-3239.doi: 10.3864/j.issn.0578-1752.2012.16.001

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS •     Next Articles

Functional Analysis via Overexpressing Xyloglucan Endotransglycosylase Gene OsXTH11 in Rice

 ZHANG  Li, NIU  Xiang-Li, ZHANG  Hui-Ying, LIU  Yong-Sheng   

  1. 1.重庆大学农学及生命科学研究院，重庆 400000
    2.合肥工业大学生物与食品工程学院，合肥 230009
  • Received:2012-03-26 Online:2012-08-15 Published:2012-05-16

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Abstract: 【Objective】 The functions of rice XTH gene (OsXTH11) in deveolpment regulation and stress responses were investigated by utilizing transgenic technology. 【Method】 The overexpression vector of OsXTH11 was constructed and introduced into rice cv. Nipponbare by Agrobacterium-mediated transformation. The phenotypes analysis and molecular detection of transgenic plants were performed to verify the function of OsXTH11. 【Result】 Real-time PCR indicated that the expression levels of OsXTH11 in transgenic lines were distinctly increased. The performance of T1 transgenic seedlings revealed that OsXTH11 overexpression lines showed increased growth rates under normal condition, and more responsive to gibberellin (GA) and auxin (IAA). The stem elongation of transgenic plants was significantly faster than that of wild type under dark or deep-water conditions, and exhibited enhanced tolerance to 100 mmol•L-1 NaCl. 【Conclusion】 Phenotypes of transgenic plants overexpressed the xyloglucan endotransglycosylase/hydrolase gene suggested the role of OsXTH11 in rice growth and salt stress.

Key words: Oryza sativa L., xyloglucan endotransglucosylase/hydrolase, phytohormone, salt stress

[1] Carpita N C, Gibeaut D M. Structural models of primary cell walls in flowering plants: Consistency of molecular structure with the physical properties of the walls during growth. The Plant Journal, 1993, 3(1): 1-30.

[2] Hayashi T, Kaida R. Function of xyloglucan in plant cells. Molecular Plant, 2011, 4(1): 17-24.

[3] Edelmann H G, Fry S C. Effect of cellulose synthesis inhibition on growth and the integration of xyloglucan into pea internode cell walls. Plant Physiology, 1992, 100(2): 993-997.

[4] Cavalier D M, Lerouxel O, Neumetzler L, Yamauchi K, Reinecke A, Freshour G, Zabotina O A, Hahn M G, Burgert I, Pauly M, Raikhel N V, Keegstra K. Disrupting two Arabidopsis thaliana xylosyltransferase genes results in plants deficient in xyloglucan, a major primary cell wall component. The Plant Cell, 2008, 20(6): 1519-1537.

[5] Vissenberg K, Fry S C, Pauly M, Hofte H, Verbelen J P. XTH acts at the microfibril-matrix interface during cell elongation. Journal of Experimental Botany, 2005, 56(412): 673-683.

[6] Fry S C. Primary cell wall metabolism: Tracking the careers of wall polymers in living plant cells. New Phytologist, 2004, 161: 641-675.

[7] Rose J K C, Braam J, Fry S C, Nishitani K. The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: Current perspectives and a new unifying nomenclature. Plant Cell Physiology, 2002, 43(12): 1421-1435.

[8] Yokoyama R, Rose J K C, Nishitani K. A surprising diversity and abundance of xyloglucan endotransglucosylase/hydrolases in rice. Classification and expression analysis. Plant Physiology, 2004, 134(3): 1088-1099.

[9] He H, Serraj R, Yang Q. Changes in OsXTH gene expression, ABA content, and peduncle elongation in rice subjected to drought at the reproductive stage. Acta Physiology Plant, 2009, 31(4): 749-756.

[10] Dong J, Jiang Y Y, Chen R J, Xu Z J, Gao X L. Isolation of a novel xyloglucan endotransgucosylase (OsXET9) gene from rice and analysis of the response of this gene to abiotic stresses. African Journal of Biotechnology, 2011, 10(76): 17424-17434.

[11] Schünmann P H D, Smith R C, Lang V, Matthews P R, Chandler P M. Expression of XET-related genes and its relation to elongation in leaves of barley (Hordeum vulgare L.). Plant Cell and Environment, 1997, 20(12): 1439-1450.

[12] Campbell P, Braam J. Xyloglucan endotransglycosylases: diversity of genes, enzymes and potential wall-modifying functions. Trends in Plant Science, 1999, 4(9): 1360-1385.

[13] Catalá C, Rose J K C, Bennett A B. Auxin-regulated genes encoding cell wall-modifying proteins are expressed during early tomato fruit growth. Plant Physiology, 2000, 122(2): 527-534.

[14] Miedes E, Lorences E P. Xyloglucan endotransglucosylase/hydrolases (XTHs) during tomato fruit growth and ripening. Journal of Plant Physiology, 2009, 166(5): 489-498.

[15] Uozu S, Tanaka-Ueguchi M, Kitano H, Hattori K, Matsuoka M. Characterization of XET-related genes of rice. Plant Physiology, 2000, 122(3): 853-859.

[16] Yokoyama R, Nishitani K. A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell wall construction in specific organs of Arabidopsis. Plant Cell Physiology, 2001, 42(10): 1025-1033.

[17] Okazawa K, Sato Y, Nakagawa T, Asada K, Kato I, Tomita E, Nishitani K. Molecular cloning and cDNA sequencing of endoxyloglucan transferase, a novel class of glycosyltransferase that mediates molecular grafting between matrix polysaccharides in plant cell walls. The Journal of Biological Chemistry, 1993, 268(34): 25364-25368.

[18] Baumann M J, Eklof J M, Michel G, Kallas A M, Teeri T T, Czjzek M, Brumer H. Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: Biological implications for cell wall metabolism. The Plant Cell, 2007, 19(6): 1947-1963.

[19] Eklof J M, Brumer H. The XTH gene family: An update on enzyme structure, function, and phylogeny in xyloglucan remodeling. Plant Physiology, 2010, 153(2): 456-466.

[20] Michailidis G, Argiriou A, Darzentas N, Tsaftaris A. Analysis of xyloglucan endotransglycosylase/hydrolase (XTH) genes from allotetraploid (Gossypium hirsutum) cotton and its diploid progenitors expressed during fiber elongation. Journal of Plant Physiology, 2009, 166 (4): 403-416.

[21] Jimenez T, Martin I, Labrador E, Dopico B. The immunolocation of a xyloglucan endotransglucosylase/hydrolase specific to elongating tissues in Cicer arietinum suggests a role in the elongation of vascular cells. Journal of Experimental Botany, 2006, 57(15): 3979-3988.

[22] Hernandez-Nistal J, Martin I, Labrador E, Dopico B. The immunolocation of XTH1 in embryonic axes during chickpea germination and seedling growth confirms its function in cell elongation and vascular differentiation. Journal of Experimental Botany, 2010, 61(15): 4231-4238.

[23] Jan A, Yang G X, Nakamura H, Ichikawa H, Kitano H, Matsuoka M, Matsumoto H, Komatsu S. Characterization of a xyloglucan endotransglucosylase gene that is up-regulated by gibberellin in rice. Plant Physiology, 2004, 136(3): 3670-3681.

[24] Hiei Y, Ohta S, Komari T, Kumashiro T. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal, 1994, 6(2): 271-282.

[25] 曹明霞, 卫志明, 黄健秋. 根癌农杆菌介导的水稻遗传转化. 植物生理学通讯, 2002, 38(5): 423-427.

Cao M X, Wei Z M, Huang J Q. Progress on transformation of rice mediated by Agrobacterium tumefaciens. Plant Physiology Communications, 2002, 38(5): 423-427. (in Chinese)

[26] Li L C, Qu R D, Kochko A D, Fauquet C, Beachy R N. An improved rice transformation system using the biolistic method. Plant Cell Reports, 1993, 12(5): 250-255.

[27] Antosiewicz D M, Purugganan M M, Polisensky D H, Braam J. Cellular localization of Arabidopsis xyloglucan endotransglycosylase- related proteins during development and after wind stimulation. Plant Physiology, 1997, 115(4): 1319-1328.

[28] Vissenberg K, Martinez-Vilchez I M, Verbelen J P, Miller J G, Fry S  C. In vivo colocalization of xyloglucan endotransglycosylase activity and its donor substrate in the elongation zone of Arabidopsis roots. The Plant Cell, 2000, 12(7): 1229-1237.

[29] Potter I, Fry S C. Xyloglucan endotransglycosylase activity in pea internodes. Plant Physiology, 1993, 103(1): 235-241.

[30] Xu W, Purugganan M M, Polisensky D H, Antoslewicz D M, Fry S C, Braam J. Arabidopsis TCH4, regulated by hormones and the environment, encodes a xyloglucan endotransglycosylase. The Plant Cell, 1995, 7(10): 1555-1567.

[31] Xu W, Campbell P, Vargheese A K, Braam J. The arabidopsis XET-related gene family: Environmental and hormonal regulation of expression. The Plant Journal, 1996, 9(6): 879-889.
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