Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (9): 1826-1832.doi: 10.3864/j.issn.0578-1752.2012.09.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

The Difference Expression Profiles of miR-181a and Transcription Regulation Region Analysis in Chicken

 SUN  Gui-Rong, LI  Ming, KANG  Xiang-Tao, LI  Guo-Xi, TIAN  Ya-Dong, HAN  Rui-Li, BAI  Yi-Chun   

  1. 1.河南农业大学牧医工程学院/河南省家禽种质资源创新工程研究中心，郑州 450002
  • Received:2011-09-07 Online:2012-05-01 Published:2011-12-01

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Abstract: 【Objective】 In order to provide theoretical data support for the study of the function of miR-181a, the tissue and developmental expression profiles, as well as transcriptional regulation region of chicken miR-181a were analyzed. 【Method】 The expression patterns of chicken miR-181a at two stages of chicken cerebrum lung, liver, spleen, kindey, crura, muscle, hupothalamus, cerebellum, heart, pectoralmuscle and thymus gland tissues were performed with qPCR technology, and the miR-181a transcriptional regulation site and the promoter region were analyzed by ENCODE. 【Result】 The results indicated that the expression of miR-181a in hypothalamus from the solexa sequencing was similar to those from qPCR. The results of qPCR showed that: the expression of miR-181a was tissue-specific in the same developmental period. The expression level of miR-181a was significantly different in different tissues. The expression in the same tissue between one day and adult chicken showed phase specific, the expression of miR-181a in other ten tissues was significantly different (P＜0.05), except liver (P＞0.05). ENCODE showed that in the upstream region of miR-181a-1 there existed continuous transcriptional regulation regions, ChIP-seq showed that the transcriptional regulatory region of miR-181a-1 may exist a transcriptional factor. The main transcription factors are NFKB, c-Fos and so on. 【Conclusion】The result indicated that the expression of miR-181a was tissue- and phase-specific. The sequence of miR-181a is highly conservative in vertebrates. The upstream of miR-181a has a transcriptional regulation region and a promoter region.

Key words: chicken, miR-181a, transcription regulation region

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