Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (8): 1576-1586.doi: 10.3864/j.issn.0578-1752.2012.08.013

• STORAGE·FRESH-KEEPING·PROCESSING • Previous Articles     Next Articles

Effects of Alginate Oligosaccharides on the Accumulation of Glyceollins in Soybean

 HU  Jia, SHI  Bo, Ojokoh Eromosele solomon , LIANG  Ping, LI  Jing-Mei   

  1. 1.中国农业科学院饲料研究所，北京 100081
    2.中国农业科学院作物科学研究所，北京 100081
  • Received:2011-11-16 Online:2012-04-15 Published:2012-03-12

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Abstract: 【Objective】The objective of this study was to investigate the relationship among alginate oligosaccharides(AOS) elicitation and glyceollins accumulation in soybean.【Method】The preparative high performance liquid chromatography was selected to separate and purify the ethanol extracts of elicited soybeans seeds with AOS, the separation process was monitored by HPLC and UPLC-MS was used to detect their molecular weights. The optimal parameters were determined by investigating the effects of the concentration of AOS, the soaking time, the temperature, moisture and time course.【Result】AOS induced glyceollins synthesis in soybean seeds. The optimal conditions of elicitation were as follows: concentration of AOS 4%, presoaked in the sterile water for 5 h, stored at a controlled temperature of 25℃ and humidity of 60% in the dark for 4 d. Results from the optimal conditions in the study indicated that the maximum concentration of the phytoalexin glyceollins was 0.525 mg•g-1 fresh weight.【Conclusion】 These findings will give some science suggestions for soybean high value processing and the development of AOS.

Key words: glyceollins, alginate oligosaccharides, elicitation, optimization

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