Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (8): 1587-1594.doi: 10.3864/j.issn.0578-1752.2012.08.014

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Genetic Diversity of Mitochondrial DNA D-loop Sequences in Ningqiang Pony

 ZHANG  Tao, LU  Hong-Chao   

  1. 1.陕西理工学院生物科学与工程学院，陕西汉中 723000
  • Received:2011-06-29 Online:2012-04-15 Published:2011-10-27

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Abstract: 【Objective】In order to provide a scientific and theoretical basis for genetic resource protection of Ningqiang pony, the genetic diversity of mitochondrial DNA (mtDNA) D-loop and maternal origin of Ningqiang pony was investigated.【Method】Twelve 600 bp mtDNA D-loop sequences were analyzed by PCR and sequencing techniques, and the phylogenetic trees were constructed based on the mtDNA D-loop sequences of Ningqiang pony samples and other equine D-loop sequence available from NCBI.【Result】There were 9 unique haplotypes with 34 polymorphic sites in Ningqiang pony, which account for 5.67% of 600 bp sequence analyzed. The haplotype diversity (h) and nucleotide diversity (π) were 0.978±0.054 and 0.01988±0.00818, respectively．Moreover, Ningqiang pony distributed in four branches, and are closely related to Hucul，Shetlan, Debao horse, Cheju horse and Mongolian horse , whereas they are less related to Thoroughbred, Yunnan horse and Jeju native horse. 【Conclusion】 Ningqiang pony have rather abundant genetic diversity and were greatly affected by other horse breeds in history.

Key words: Ningqiang pony, mitochondrial DNA D-loop, genetic diversity, maternal origine

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