Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (8): 1465-1474.doi: 10.3864/j.issn.0578-1752.2012.08.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Creative Process of a New Restorer Material with Ogu CMS in Brassica nupus

 CHEN  Wei-Jiang, LI  Mei, WANG  Tong-Hua, HUI  Rong-Kui, TU  Jin-Xing, FU  Ting-Dong   

  1. 1.湖南省农业科学院作物研究所，长沙 410125
    2.华中农业大学植物科学技术学院，武汉 430070
  • Received:2011-12-31 Online:2012-04-15 Published:2012-02-07

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Abstract: 【Objective】 The objective of this study was to explore the radish restore gene transferring and the stable restorer line screening for Ogu CMS in rapeseed.【Method】 The Raphanobrassica (2n=58) as the restoring gene donor and grafting as the restoring gene importing tool was used to obtain the hybrid of Ogu CMS in B. napus. And the stable restoring  materials were bred through selection of the seed setting rate in single plant and the restoring ratio in populations from every selfing and backcross generations. Also the selected materials were detected by the cytological observation and the molecular marker screening. 【Result】With the high strength selection, a stable homozygous restoring line named as CLR650 of Ogu CMS in B. napus was selected. This line can restore the male fertility in both progenies of self-pollination and testcross at a rate of 100%. Although it’s somatic chromosome numbers were in 2n= 38 to 40 and some abnormal phenomena such as chromosome bridge and lagged chromosomes in meiosis were observed, the pollens still could be normally formed without the influence on the normal seed setting. It was proved that the Ogu CMS restoring gene existed in CLR650 and the length of radish fragment was shorter than the Ogu CMS restoring line R113 by detecting the molecular markers which closely linked with radish restoring gene (Rf0). 【Conclusion】 The restoring line CLR650 could be a new restoring type for the Ogu CMS in B. napus.

Key words: Brassica napus, Raphanobrassica, grafting, wide hybridization, Ogu CMS restorer

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