Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (6): 1191-1198.doi: 10.3864/j.issn.0578-1752.2012.06.018

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Influence of 5-HT Transporter Inhibitors on the 5-HT Content and the Proliferation of Smooth Muscle Cells in Pulmonary Arteriolar Media in Broilers with Pulmonary Hypertension

 LI  Ying, ZENG  Jian-Ying, HAN  Qian-Biao, TANG  Zhao-Xin, GUO  Jian-Ying, PAN  Jia-Qiang   

  1. 华南农业大学兽医学院，广州 510642
  • Received:2011-10-11 Online:2012-03-15 Published:2012-01-09

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Abstract: 【Objective】 An experiment was designed to detect the relationship between the content of 5-hydroxytryptamine (5-HT) in the media layer of pulmonary arterioles and the proliferation of pulmonary arterioles smooth muscle cells, with the purpose to clarify whether or not 5-HT transporter (5-HTT) was participated in the occurrence of pulmonary hypertension in broilers.【Method】Broilers at 20 day of age were randomly divided into non-disease inducing groups (intravenously injection of normal saline, including blank control group, Fluoxetine group, Citalopram group, 30 fowls in each group) and disease inducing groups (intravenously injection of cellulose particles, including medicine blank control group, Fluoxetine group, Citalopram group, 50 fowls in each group.). From day 21 on, the morbidity of pulmonary hypertension syndrome (PHS) of each group were recorded. Samples were randomly taken from each group on day 21, 28, 35 and 42, and the weight ratio between right ventricle and total ventricle (RV and TV) was determined. 5-HT content in pulmonary arteriolar media layer was detected by using the immunohistochemical technique, and the proliferation conditions of smooth muscle cells in pulmonary arterioles was detected by argyrophilic nucleolar organizer region proteins staining method (AgNOR) and proliferating cell nuclear antigen (PCNA) staining method.【Result】PHS morbidity and RV/TV value of disease-inducing groups were significantly higher than that of non-disease inducing groups (P＜0.05). The 5-HT content in pulmonary arteriolar media was increased in disease-inducing groups, as well as the argyrophilic particles on the smooth muscle cells and the PCNA positive cells. Fluoxetine and Citalopram (both are 5-HTT inhibitors) significantly reduced the morbidity of PHS and 5-HT contents in pulmonary arteriolar media in broiler, and inhibited the proliferation of smooth muscle cells. There were no significant difference between the two medicines.【Conclusion】During the occurrence of pulmonary hypertension in broilers, the increasing of 5-HT content in pulmonary arteriolar smooth muscle cells which was mediated by 5-HTT resulted in the  excessive proliferation of smooth muscle cells, leading to the occurrence of PHS. 5-HTT is possibly a significant target molecular for the prevention and treatment of PHS in broilers.

Key words: pulmonary hypertension, smooth muscle cells, 5-hydroxytryptamine, 5-hydroxytryptamine transporter inhibitor, broiler

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