Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (6): 1199-1205.doi: 10.3864/j.issn.0578-1752.2012.06.019

• VETERINARY SCIENCE • Previous Articles     Next Articles

Detection of High Pathogenicity Islands in Escherichia coli Isolated from Clinical and Subclinical Bovine Mastitis in Some Areas of China

 XU  Ji-Ying, YANG  Zhi-Qiang, CHEN  Hua-Qi, LIU  Jun-Lin, XING  Juan, LI  Jian-Xi, LI  Hong-Sheng   

  1. 1.西北民族大学生命科学与工程学院，兰州 730000
    2.中国农业科学院兰州畜牧与兽药研究所，兰州 730050
    3.中国农业科学院兰州兽医研究所，兰州 730000
  • Received:2011-09-30 Online:2012-03-15 Published:2012-02-06

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Abstract: 【Objective】The objective of the experiment is to investigate the present distribution of high pathogenicity island (HPI) in E. coli isolated from cows with clinical and subclinical mastitis in China.【Method】Milk samples from 1 260 cows with clinical and subclinical mastitis in different farms and areas were used to screen Gram-negative bacteria by MacConkey agar. One hundred and ninety E. coli isolates was further identified from the Gram-negative bacteria by microbiological tests. Total DNA extraction from E. coli was used to detect HPI genes by PCR.【Result】The PCR results showed that 50 out of 190 isolates carried the irp2 gene, 36 isolates were positive for fyuA gene and 32 for intB genes. Sequence analysis of randomly selected PCR products showed that homology of genes irp2, fyuA, and asn_tRNA_intB were above 97.1%, 98.2%, and 97.2% identical to the published sequences. HPI+ isolates were associated with fyuA (72%) and asn_tRNA locus (64%).【Conclusion】 Yersinia HPI was widely distributed in the E. coli isolates from cows with clinical and subclinical mastitis in China. The prevalence of HPI is associated with special serotypes in E.coli from cows with clinical and subclinical mastitis.

Key words: bovine mastitis, Escherichia coli, high pathogenicity island (HPI), O serogroups

[1]Carniel E, Guilvout I, Prentice M. Characterization of a large chromosomal ‘high_pathogenicity island’ in biotype 1B Yersinia enterocolitica. Journal of Bacteriology, 1996, 178(23): 6743-6751.

[2]Buchrieser C, Prentice M, Carniel E. The 102-kilobase unstable region of Yersinia pestis comprises a high-pathogenicity island linked to a pigmentation segment which undergoes internal rearrangement. Journal of Bacteriology, 1998a, 180: 2321-2329.

[3]Rakin A, Noelting C, Schubert S, Heesmann J. Common and specific characteristics of the high-pathogenicity island of Yersinia enterocolitica. Infection and Immunity, 1999, 67(10): 5265-5274.

[4]Mokracka J, Koczura R, Kaznowski A. Yersiniabactin and other siderophores produced by clinical isolates of Enterobacter spp. and Citrobacter spp. FEMS Immunology & Medical Microbiology, 2004, 40(1): 51-55.

[5]Carniel E. The Yersinia high-pathogenicity island: an iron-uptake island. Microbes and Infection, 2001, 3(7): 561-569.

[6]Rakin A, Urbitsch P, Heesemann J. Evidence for two evolutionary lineages of highly pathogenic Yersinia species. Journal of Bacteriology, 1995, 177(9): 2292-2298.

[7]Bach S, de Almeida A, Carniel E. The Yersinia high- pathogenicity island is present in different members of the family Enterobacteriaceae. FEMS Microbiology Letters, 2000, 183(2): 289-294.

[8]Rangel P M, Marin J M. Antimicrobial resistance in Brazilian isolates of Shiga toxin-encoding Escherichia coli from cows with mastitis. Ars Veterinaria, Jaboticabal, SP, 2009, 25 (1): 18-23.

[9]Sumathi B R, Gomes A R, Krishnappa G. Antibiogram profile based dendrogram analysis of Escherichia coli serotypes isolated from bovine mastitis. Veterinary World, 2008, 1(2): 37-39.

[10]王  娜, 高学军. 哈尔滨地区奶牛隐性乳房炎病原菌的分离鉴定. 东北农业大学学报, 2011, 42(2): 29-32.

Wang N, Gao X J. Isolation and identification of pathogenic bacteria of bovine subclinical mastitis in Harbin. Journal of Northeast Agricultural University, 2011, 42(2): 29-32. (in Chinese)

[11]金兰梅, 伍清林, 马高民, 葛继文, 丁  艳, 赵  超, 王  君. 规模化奶牛场奶牛乳房炎病原菌的分离与药敏试验. 黑龙江畜牧兽医, 2010, 42(21): 101-103.

Jin L M, Wu Q L, Ma G M, Ge J W, Ding Y, Zhao C, Wang J. Isolation and Identification on Pathogens from cow mastitis in scale cow farms and their drug sensitive experiment. Heilongjiang Animal Science and Veterinary Medicine, 2010, 42(21): 101-103. (in Chinese)

[12]Burvenich C, Van Merris V, Mehrzad J, Diez-Fraile A, Duchateau L. Severity of E. coli mastitis is mainly determined by cow factors. Veterinary Reseach, 2003, 34 (5): 521-564.

[13]Lucier T S, Fetherston J D, Brubaker R R, Perry R D. Iron uptake and iron-repressible polypeptides in Yersinia pestis. Infection and Immunity, 1996, 64(8): 3023-3031.

[14]Koczura R, Kaznowski A. The Yersinia high-pathogenicity island and ironuptake systems in clinical isolates of Escherichia coli. Journal of Medical Microbiology, 2003, 52: 637-642.

[15]Brem D, Pelludat C, Rakin A, Jacobi C A, Heesemann J. Functional analysis of yersiniabactin transport genes of Yersinia enterocolitica. Microbiology, 2001, 147(5): 1115-1127.

[16]Schubert S, Rakin A, Heesmann J. The Yersinia high- pathogenicity island (HPI): evolutionary and functional aspects. International Journal of Medical Microbiology, 2004, 294(2): 83-94.

[17]Fetherston J. D, Lillard J. W, Perry R. D. Analysis of the Pesticin Receptor from Yersinia pestis: Role in Iron-Deficient Growth and Possible Regulation by Its Siderophore. Journal of Bacteriology, 1995: 1824-1833.

[18]Rakin A, Schubert S, Guilvout I. Local hopping of IS3 elements into the A+T rich part of the high-pathogenicity island in Yersinia enterocolitica1B, O: 8. FEMS Microbiology Letters, 2000, 182(2): 225-229.

[19]Clermont O, Bonacorsi S, Bingen E. The Yersinia high- pathogenicity island is highly predominant in virulence- associated phylogenetic groups of Escherichia coli. FEMS Microbiology Letters, 2001, 196(2): 153-157.

[20]Penteado A S, Ugrinovich L A, Blanco J. Serobiotypes and virulence genes of Escherichia coli strains isolated from diarrheic and healthy rabbits in Brazil. Veterinary Microbiology, 2002, 89(1): 41-51.

[21]叶长芸, 徐建国. 部分ESIEC菌株存在耶尔森氏菌HPI毒力岛. 疾病监测, 2000, 15(2): 48-50.

Ye C Y, Xu J G. A “High-pathogenicity Island” of Yersinia enterocolitia exsited in entero-SLT-producing and invasive E. Coli strains. Disease Surveillance, 2000, 15(2): 48-50. (in Chinese)

[22]王 勇, 王 红, 向 前, 孙素霞, 俞守义. 产毒性和致病性大肠埃希菌中小肠结肠炎耶尔森菌强毒力岛的检测. 第一军医大学学报, 2002, 22(7): 580-583.

Wang Y, Wang H, Xiang Q, Sun S X, Yu S Y. Detection of the high-Pathogenieity island of Yersinia enterocolitica in enterotoxigenic and enteroPathogenic E. coli strains. Journal of First Military Medical University, 2002, 22(7): 580-583. (in Chinese)

[23]金文杰, 郑志明, 秦爱建, 刘岳龙, 邵红霞, 张永志, 黄训良. 禽致病性大肠杆菌中耶尔森菌强毒力岛的分子流行病学调查. 中国兽医科学, 2006, 36(10): 787-790.

Jin W J, Zheng Z M, Qin A J, Liu Y L, Shao H X, Zhang Y Z, Huang X L. Molecular epidemiological investigation of Yersinia high- pathogenicity island in avian pathogenic Escherichia coli. Veterinary Science in China, 2006, 36(10): 787-790. (in Chinese)

[24]陈  祥, 赵  娟, 高  崧, 王  雷, 焦新安, 刘秀梵. 我国部分地区猪源大肠杆菌LEE和HPI毒力岛相关基因的检测. 中国人兽共患病学报, 2006, 22(1): 33-47.

Cheng X, Zhao J, Gao S, Miao X Q, Liu J, Huang L L, Jiao X A, Liu X F. Detection of related genes of LEE and HPI pathogenicity island of Escherichia coli strains isolated from pigs in China. Chinese Journal of Zoonoses, 2006, 22(1): 33-47. (in Chinese)

[25]成大荣, 孙怀昌, 徐建生, 高  崧. 断奶仔猪源大肠杆菌LEE及HPI毒力岛的检测. 微生物学报, 2006, 46(3): 368-372.

Cheng D R, Sun H C, Xu J S, Gao S. Prevalence of LEE and HPI pathogenicity islands of Escherichia coli isolates from weaned piglets in China. Acta Microbiologica Sinica, 2006, 46(3): 368-372. (in Chinese)

[26]Schubert S, Rakin A, Fischer D, Sorsa J, Heesmann J. Characterization of the integration site of Yersinia high- pathogenicity island in Escherichia coli. FEMS Microbiology Letters, 1999, 179(2): 409-414.

[27]Rakin A, Noelting C, Schropp P, Heesemann J. Integrative module of the high-pathogenicity island of Yersinia. Molecular Microbiology, 2001, 39(2): 407-416.

[28]Karch H, Schubert S, Zhang D, Zhang W, Schmidt H, Olschlager T, Hacker J. A Genomic island, termed high- pathogenicity island, is present in certain non-O157 shiga toxin-producing Escherichia coli Clonal Lineages. Infection and Immunity, 1999, 67(11): 5994-6001.

[29]Schubert S, Picard B, Gouriou S, Heesemann J, Denamur E. Yersinia High-pathogenicity island contributes to virulence in Escherichia coli causing extraintestinal infections. Infection and Immunity, 2002, 70(9): 5335-5337.
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