Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (6): 1183-1190.doi: 10.3864/j.issn.0578-1752.2012.06.017

• ANIMAL SCIENCE·RESOURCE INSECT • Previous Articles     Next Articles

Cloning of Dairy Goat Prm1 Gene CDS and Construction of the Eukaryotic Expression Vector

 LI  Ming-Zhao, LIU  Chao, SUN  Jun-Wei, ZHU  Hai-Jing, CAO  Hui, LU Xiao , CHOU  Pu-Bin, HUA  Jin-Lian   

  1. 西北农林科技大学动物医学院/陕西省干细胞工程技术研究中心/农业部动物生物技术重点开放性实验室，陕西杨凌 712100
  • Received:2011-05-09 Online:2012-03-15 Published:2011-08-18

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Abstract: 【objective】The objective of the experiment is to study the Prm1 gene of dairy goat, and for further research on dairy goat spermatogenesis.【Method】The CDS of Prm1 gene was cloned by PCR from Guanzhong dairy goat testis tissue. The obtained sequence was constructed into the eukaryotic expression vector pIRES-GFP, and then transfected to GC1 cell line and human umbilical cord cells. PCR and immunohistochemistry were used to test the expression of Prm1 at different ages of dairy goat testis. 【Result】In dairy goats, the expression of Prm1 gene was high and specific in adult testis, located only in the head of sperm. The CDS of Prm1 gene of Guanzhong dairy goat was amplified and compared with the sequences of other species. The results showed that they shared a high homology, being 97.4% between dairy goats and bovine. The Prm1 gene was overexpressed in GC1 cells and human umbilical cord cells to confirm its function. 【Conclusion】 The complete CDS of dairy goat Prm1 gene and its expression patterns were obtained.

Key words: Guanzhong dairy goat, Prm1 gene, gene cloning, expression pattern

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