Scientia Agricultura Sinica ›› 2012, Vol. 45 ›› Issue (3): 584-589.doi: 10.3864/j.issn.0578-1752.2012.03.022

• RESEARCH NOTES • Previous Articles     Next Articles

Effect of Salicylic Acid Dipping on Postharvest Diseases and Phenylpropanoid Pathway in Muskmelon Fruits

 FAN  Cun-Fei, BI  Yang, WANG  Yun-Fei, REN  Ya-Lin, YANG  Zhi-Min, WANG  Yi   

  1. 1.甘肃农业大学食品科学与工程学院,兰州730070
  • Received:2011-09-30 Online:2012-02-01 Published:2011-11-28

Abstract: 【Objective】The effects of salicylic acid (SA) dipping on postharvest disease resistance and phenylpropanoid pathway in muskmelon fruit was investigated in this paper. 【Method】 Muskmelons (Cucumis melo L. cv.Yujinxiang) were dipped at 4 mmol•L-1 of SA for 10 min. The inhibition effect of SA dipping on the lesion diameter of fruits inoculated with Trichothecium roseum and natural incidence of melons were investigated during storage at room temperature. The key enzyme activities and substances accumulation of phenylpropanoid pathway in fruits were also detected. 【Result】 SA significantly decreased the lesion diameter of fruits inoculated with T. roseum, and the natural incidence of fruits during storage at room temperature. The treatment increased the activity of phenylalanine ammonia-lyase (PAL), 4-coumarate: CoA ligase (4CL) and cinnamate-4hydroxylase (C4H) in fruits. The activity of C4H and 4CL was 1.0 and 2.8 times higher in treated fruits than that in the control after 6 days of treatment, and PAL activity was 1.6 times of the control after 10 days of treatment. SA significantly enhanced the content of total phenolic and lignin. The content of lignin and total phenolic was 30.6% and 30.0% higher in treated fruits than that in the control after 4 and 10 days of treatment; however, no significant difference was found in flavanoid content between the treatment and the control. 【Conclusion】Postharvest SA treatment could increase the resistance in melon fruits against postharvest diseases by inducing phenylpropanoid pathway.

Key words: muskmelon, salicylic acid, phenylpropanoid pathway, postharvest diseases

[1]Bi Y, Ge Y H, Wang C L, Li X W. Melon production in China. Acta Horticulturae, 2007, 731(1): 493-500.

[2]葛永红, 毕  阳, 杨冬梅. 诱抗剂处理对“银帝”甜瓜采后粉霉病和黑斑病的抑制效果. 食品科学, 2006, 27(1) : 246-249.

Ge Y H, Bi Y, Yang D M. Effect of elicitors treatment on postharvest Trichothecium and Alternaria inoculation in harvested muskmelon (cv.Yindi). Food Science, 2006, 27(1): 246-249. (in Chinese)

[3]马凌云, 毕  阳, 张正科, 赵  亮, 安  力, 马克奇. 采前嘧菌酯处理对“银帝”甜瓜采前及采后主要病害的控制. 甘肃农业大学学报, 2004, 39(1): 14-17.

Ma L Y, Bi Y, Zhang Z K, Zhao L, An L, Ma K Q. Control of pre- and postharvest main diseases on melon variety Yindi with preharvest azoxystrobin spraying. Journal of Gansu Agricultural University, 2004, 39(1): 14-17. (in Chinese)

[4]Terry L A, Joyce D C. Elicitors of induced disease resistance in postharvest horticultural crops: a brief review. Postharvest Biology and Technology, 2004, 32:1-13.

[5]张咏梅, 安  力, 毕  阳, 柴世龙, 马克奇. BTH对厚皮甜瓜过氧化物酶、几丁质酶活性和木质素积累的影响. 甘肃农业大学学报, 2005, 40(3): 315-318.

Zhang Y M, An L, Bi Y, Chai S L, Ma K Q. Effect of BTH treatment on peroxidase, chitinase activities and lignin accumulation of muskmelons. Journal of Gansu Agricultural University, 2005, 40(3): 315-318. (in Chinese)

[6]Malamy J, Carr J P, Klessing D F, Daniel F, Raskin I. Salicylic acid: a likely endogenous signal in the resistance response of tobacco to viral infection. Science, 1990, 250: 1002-1004.

[7]Gaffney T, Friedrich L, Vernooij B, Negrotto D, Nye G, Uknes S, Ward E, Kessmann H, Ryals J. Requirement of salicylic acid for the induction of systemic acquired resistance. Science, 1993, 261(5122): 754-756.

[8]曾凯芳, 姜微波. 水杨酸处理对采后绿熟芒果炭疽病抗病性的诱导. 中国农业大学学报, 2005, 10(2): 36-40.

Zeng K F, Jiang W B. Induced resistance to anthracnose (Colletotrichum gloeosporioides) in mango fruit by postharvest treatment with salicylic acid. Journal of China Agricultural University, 2005, 10(2): 36-40. (in Chinese)

[9]王芳芳. 水杨酸处理对苹果轮纹病的抑制效果. 北方园艺, 2010(3): 147-148.

Wang F F. The inhibitory effect on the apple physalospora after treated with salicylic acid. Northern Horticulture, 2010(3): 147-148. (in Chinese)

[10]汤晓莉, 薛红芬, 邓国宾, 杨明挚, 王燕云, 陈小兰. 水杨酸诱导马铃薯疮痂病抗性的生理机制研究. 西南农业学报, 2010, 23(6): 1851-1854.

Tang X L, Xue H F, Deng G B, Yang M Z, Wang Y Y, Chen X L. Physiological mechanism of salicylic acid induced resistance to common scab of potato. Southwest China Journal of Agricultural Sciences, 2010, 23(6): 1851-1854. (in Chinese)

[11]Yao H J, Tian S P. Effects of pre- and post-harvest application of salicylic acid or methyl jasmonate on inducing disease resistance of sweet cherry fruit in storage. Postharvest Biology and Technology, 2005, 35: 253-262.

[12]曾凯芳. 套袋、SA和INA对芒果(Mangifera indica L.)果实采后抗病性和品质的影响[D]. 北京:中国农业大学, 2005.

Zeng K F. Effects of bagging, SA and INA on postharvest quality and disease resistance of mango (Mangifera indica L.) fruit[D]. Beijing: China Agricultural University, 2005. (in Chinese)

[13]曹建康. SA、ASM、INA和柠檬酸对鸭梨果实采后抗病性和品质的影响[D]. 北京: 中国农业大学, 2005.

Cao J K. Effects of SA, ASM, INA and citric acid on postharvest disease resistance and quality of Yali pear fruit[D]. Beijing: China Agricultural University, 2005. (in Chinese)

[14]Bi Y, Tian S P, Zhao J, Ge Y H. Harpin induces local and systemic resistance against Trichothecium roseum in harvested Hami melons. Postharvest Biology Technology, 2005, 38: 183-187.

[15]Liu H X, Jiang W B, Bi Y, Luo Y B. Postharvest BTH treatment induces resistance of peach (Prunus persica L. cv. Jiubao) fruit to infection by Penicillium expansum and enhances activity of fruit defense mechanisms. Postharvest Biology and Technology, 2005, 35: 263-269.

[16]Lamb C J, Rubery P H. A spectrophotometric assay for trans-cinnamic acid 4-hydroxylase activity. Analytical Biochemistry, 1975, 68: 554-561.

[17]朱明华, 欧阳光察, 薛应龙. 黄瓜免疫诱导过程中G6PD、PAL、4CL、PO活性和木质素含量的变化. 上海农业学报, 1990, 6(2): 21-26.

Zhu M H, Ouyang G C, Xue Y L. Changes of activities of four enzymes (G6PD, PAL, 4CL, PO) and lignin content in immunization-induced cucumber. Acta Agriculturae Shanghai, 1990, 6(2):21-26. (in Chinese)

[18]Pirie A, Mullins M G. Changes in anthocyanin and phenolics content of grapevine leaf and fruit tissue treated with sucrose, nitrate, and abscisic acid. Plant Physiology, 1976, 58: 468-472.

[19]刘国强, 吴锦程, 唐朝晖, 张琳华, 戴巧斌. 枇杷采后酚类物质代谢与果肉木质化的关系. 中国农学通报, 2008, 24(1): 247-251.

Liu G Q, Wu J C, Tang C H, Zhang L H, Dai Q B. Relationship between phenols compounds metabolism and pulp lignification of postharvest loquat. Chinese Agricultural Science Bulletin, 2008, 24(1): 247-251. (in Chinese)

[20]曹建康, 毕  阳, 李永才, 赵  劼. 水杨酸处理对苹果梨采后黑斑病及贮藏品质的影响. 甘肃农业大学学报, 2001, 36(4): 438-442.

Cao J K, Bi Y, Li Y C, Zhao J. Effects of salicylic acid treatment on the postharvest black spot disease and quality of Pingguoli pear during storage. Journal of Gansu Agricultural University, 2001, 36(4): 438-442. (in Chinese)

[21]Qin G Z, Tian S P, Xu Y, Wan Y K. Enhancement of biocontrol efficacy of antagonistic yeasts by salicylic acid in sweet cherry fruit.  

Physiological and Molecular Plant Pathology, 2003, 62(3): 147-154.

[22]Hammerschmidt R. Induced disease resistance: how do induced plants stop pathogens? Physiological and Molecular Plant Pathology, 1999, 55: 77-84.

[23]Mauch-Mani B, Slusarenko A J. Production of salicylic acid precursors is a major function of phenylalanine ammonia-lyase in the resistance of Arabidopsis to Peronospora parasitica. The Plant Cell, 1996, 8: 203-212.

[24]Whetten R, Sederoff R. Lignin biosynthesis. The Plant Cell, 1995, 7: 1001-1013.

[25]李  莉, 赵  越, 马君兰. 苯丙氨酸代谢途径关键酶: PAL、C4H、4CL研究新进展. 生物信息学, 2007, 5(4): 187-189

Li L, Zhao Y, Ma J L. Recent progress on key enzymes: PAL, C4H, 4CL of phenylalanine metabolism pathway. China Journal of Bioinformatics, 2007, 5(4): 187-189. (in Chinese)

[26]张维一, 毕  阳. 果蔬采后病害与控制. 北京: 中国农业出版社, 1996.

Zhang W Y, Bi Y. Postharvests Diseases and Control of Fruits and Vegetables. Beijing: China Agriculture Press, 1996. (in Chinese)

[27]赵淑娟, 刘  涤, 胡之璧. 植物4-香豆酸:辅酶A连接酶. 植物生理学通讯, 2006, 42(3): 529-538.

Zhao S J, Liu D, Hu Z B. 4-coumarate: Coenzyme a ligase in plant. Plant Physiology Communications, 2006, 42(3): 529-538. (in Chinese)

[28]Reimers P J, Leach I E. Race-specific resistance to Xanthomonas oryzae pv. Oryzae conferred by bacterial blight resistance gene Xa-10 in rice (Oryzae sativa) involves accumulation of a lignin-like substance in host tissues. Molecular Plant Pathology, 1999, 38: 39-55.

[29]罗建平, 夏  宁, 沈国栋. 茉莉酸甲酯、水杨酸和一氧化氮诱导怀槐悬浮细胞合成异黄酮及细胞结构变化. 分子细胞生物学报, 2006, 39(5): 438-444.

Luo J P, Xia N, Shen G D. Isoflavone accumulation associated with cell structural changes in Maackia amurensis suspension cultures elicited by methyl jasmonate, salicylic acid and nitric oxide. Journal of Molecular Cell Biology, 2006, 39(5): 438-444. (in Chinese)

[30]赵  艳, 李  昂, 徐茂军. 前处理刺激采摘后芦笋中类黄酮积累的研究. 食品研究与开发, 2006, 127(7): 42-44.

Zhao Y, Li A, Xu M J. Study on the flavonoids accumulation in harvested asparagus by pretreatment stimulation. Food Research and Development, 2006, 127(7): 42-44. (in Chinese)
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