Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (18): 3750-3758.doi: 10.3864/j.issn.0578-1752.2011.18.006

• PLANT PROTECTION • Previous Articles     Next Articles

Physiological Effect and Cytological Characterization Regarding Susceptible Response of New Herbicide ZJ0273 in Barley

 GUO  Xiang, ZHANG  Fan, JIN  Zong-Lai, DENG  Xiang-Qin, WU  Ling-Tong, Warusawitharana  Hasitha, ZHOU  Wei-Jun   

  1. 1.浙江大学作物科学研究所
  • Received:2010-11-18 Revised:2011-06-13 Online:2011-09-15 Published:2011-07-27

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Abstract: 【Objective】In order to define the safety and mechanism of new herbicide propyl 4-(2-(4,6-dimethoxypyrimidin- 2-yloxy)benzylamino) benzoate (ZJ0273) to barley, this paper investigated the physiological effect of ZJ0273 on the root-tip cell ultrastructural development and mitosis in barley. 【Method】 Effects of different concentrations of ZJ0273 applied at 4th leaf stage on acetolactate synthase (ALS) activity, protein content, photosystem II quantum characteristics, SPAD, net photosynthesis (Pn) and 100 mg•L-1 ZJ0273 on the development of root-tip cell were analyzed by various physiological measurements and microscopy techniques. 【Result】The inhibited effect of 100 mg•L-1 ZJ0273 treatment (also a recommended concentration used in the field) on the growth of barely seedlings was slight at first, but this treatment inhibited significantly the ALS activity, protein content, photosynthetic system of barley seedlings along with the prolongation of treatment durations. The results also showed that the volume and number of vacuoles as well as mitotic index in treated cells were decreased, while the number of microbodies was increased. Both of 500 and 1 000 mg•L-1 ZJ0273 treatments inhibited the physiological index of barley at different treatment times. 【Conclusion】 Barley seedlings are sensitive to herbicide ZJ0273 at concentration of 100 mg•L-1at late stage and unsafe at 500 and 1000 mg•L-1. The inhibitive effect of ZJ0273 on photosystem II and the change of microbody in mitochondria might be related to the mechanism of this new herbicide.

Key words: barley, ZJ0273, acetolactatesynthase(ALS), proteincontent, photosystem, microscopicstructure, mitoticindex

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