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Evolution and Genetic Recombination of Physiological Races of Puccinia striiformis f. sp. tritici in China

ZHAN Gang-ming, WANG Jian-feng, WANG Xiao-jie, HUANG Li-li, KANG Zhen-sheng   

  1. College of Plant Protection, Northwest Agriculture and Forestry University/Key Laboratory of Molecular Biology for Agriculture of Shaanxi, Yangling 712100, Shaanxi
  • Received:2010-10-20 Online:2011-05-05 Published:2011-02-28

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Abstract: 【Objective】 The objective of this study is to explore the virulence evolution and relative relationship in main physiological races of Puccinia striiformis f. sp. tritici (Pst) in China, for searching the molecular evidence of somatic genetic recombination and revealing the virulence variation mechanism of physiological races of Pst in China.【Method】Virulence of 21 races collected during the past 12 years were tested on Chinese differentials, and then the genomic DNA isolated from them were used for SSR analysis by TP-M13-SSR technique. 【Result】 Twenty-one races were clustered into 4 groups in both virulent analysis and SSR markers, the correlation index between them is 0.21. Furthermore, 2 pairs of primers among 13 pairs showed the existence of recombinant among the physiological races.【Conclusion】The Chinese physiological races of Pst have abundant diversity of virulence, but the genetic divergence among them are low, the somatic recombination of urediospores acts as a possible major mechanism of virulence variation for Pst in China.

Key words: Puccinia striiformis f. sp. tritici , virulent analysis , SSR marker , genetic recombination

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