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Detection of Apoptosis in Homocaryon with C and N Cytoplasm of Maize Induced by Helminthosporium maydis Race C (HMC) Toxin

MA Chun-hong; LI Xiu-li;DONG Wen-qi; ZHANG Hong-xin; LI Yun-chao; CUI Si-ping; WANG Li-an; JIA Yin-suo; DAI Zhi-gang   

  1. 1、Institute of Genetics and Physiology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051;
    2、College of Life Science, Hebei Normal University, Shijiazhuang 050016;
    3、Department of Science and Technology, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051;
    4、Tangshan Teachers College, Tangshan 063000, Hebei；5Soil and Fertilizer Station of Hubei Province, Wuhan 430064
  • Received:2010-07-15 Online:2011-05-05 Published:2010-09-27

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Abstract: 【Objective】 To study the feature of apoptosis and the change of its ratio induced by HMC toxin in the root cap cells detached from a pair of homokaryon Mo17-C and Mo17-N inbred line of maize. 【Method】AO and EB staining and Hoechst 33258 staining methods were used to detach the apoptosis of root cap cells. In the process of induction, root cap cells appeared apoptotic bodies and marginate of chromosome morphology. Living, necrosis and apoptosis cells could be distinguished clearly by AO and EB complex staining and Hoechst 33258 staining. 【Result】The ratio of apoptosis in Mo17-C cells was sharply higher than that of Mo17-N, and the max ratios of apoptosis of Mo17-C and Mo17-N were 68.7% and 29%. In contrast, the max ratios of apoptosis of Mo17-C and Mo17-N stained by Hoechst 33258 were 57.3% and 27.7%, respectively. After HMC toxin with 150 μg·mL-1 treated for 7 h, the apoptosis rate was the highest. 【Conclusion】 Harmed by HMC toxin, the root cap cells appeared apoptosis. The ratio of apoptosis in C cytoplasm was higher than in N cytoplasm. Along with the increase of toxin concentration and treating time, the ratio of apoptosis became higher. In spite of the different staining methods by AO and EB or by Hoechst 33258, the ratio of the cell apoptosis was similar. But the early and late apoptotic cells could be distinguished only by AO and EB complex staining.

Key words: Helminthosporium maydis (Bipolaris maydis) race C (HMC) , toxin , apoptosis , Hoechst 33258 , AO , EB

[1]罗孟军, 朱天辉. 植物病原真菌毒素. 四川林业科技, 2001, 22(3): 45-49.
Luo M J, Zhu T H. Phytotoxin in plant pathogen. Journal of Sichuan Forestry Science and Technology, 2001, 22(3): 45-49. (in Chinese)
[2]刘克明, 吴全安, 刘俊芳, 梁克恭, 魏建昆. 玉米小斑病菌三个生理小种生物学特性比较的初步研究. 华北农学报, 1989, 4(2): 74-78.
Liu K M, Wu Q A, Liu J F, Liang K G, Wei J K. Preliminary studies on comparative biological traits of three races of Bipolaris maydis. Acta Agriculturae Boreali-Sinica, 1989, 4(2): 74-78. (in Chinese)
[3]Wei J K, Liu K M, Chen J P, Luo P C, Lee-Stadelmann O Y. Pathological and physiological identification of race C of Bipolaris maydis in China. Phytopathology, 1988, 78(5): 550-554.
[4]Wei J K, Zhao K M, Deng L Y, Lee-Stadelmann O Y. Protoplasmic parameters of Zea mays with normal and male sterile cytoplasm. Physiological and Molecular Plant Pathology, 1989, 35(6): 543-553.
[5]郭兰凯, 刘俊芳, 马春红, 崔  洋. HMC毒素对雄花不育及正常细胞质玉米根冠活细胞的影响. 作物学报, 1991, 17(1): 54-57.
Guo L K, Liu J F, Ma C H, Cui Y. Effect of HMC toxin on the living root cap cells of maize with Cms and N cytoplasm. Acta Agronomica Sinica, 1991, 17(1): 54-57. (in Chinese)
[6]商  闯, 马春红, 李运朝, 翟彩霞, 董文琦, 崔四平, 侯立白, 贾银锁. 低浓度玉米小斑病菌C小种毒素培养滤液对玉米叶片过氧化物酶活性的诱导作用. 中国生物防治, 2007, 23(增刊): 42-46.
Shang C, Ma C H, Li Y C, Zhai C X, Dong W Q, Cui S P, Hou L B, Jia Y S. The induced effects of filtrate of Bipolaris maydis race C toxin cultivation on POD activities in maize leaves. Chinese Journal of Biological Control, 2007, 23(Suppl.): 42-46. (in Chinese)
[7]商  闯, 马春红, 李运朝, 翟彩霞, 董文琦, 崔四平, 侯立白, 贾银锁. 玉米小斑病菌C毒素培养滤液对玉米叶片苯丙氨酸解氨酶(PAL)活性的诱导效应. 玉米科学, 2008, 16(2): 131-134.
Shang C, Ma C H, Li Y C, Zhai C X, Dong W Q, Cui S P, Hou L B, Jia Y S. The induced effects of filtrate of Bipolaris maydis race C toxin cultivation on PAL activities in maize leaves. Journal of Maize Sciences, 2008, 16(2): 131-134. (in Chinese)
[8]商  闯, 马春红, 翟彩霞, 李运朝, 董文琦, 崔四平, 侯立白, 贾银锁. 丙二醛(MDA)含量在玉米诱导抗病过程中的变化. 华北农学报, 2007, 22(增刊): 29-32.
Shang C, Ma C H, Zhai C X, Li Y C, Dong W Q, Cui S P, Hou L B, Jia Y S. The changes of malondialdenyde content on course of the induced disease-resistance. Acta Agriculturae Boreali-Sinica, 2007, 22(Suppl): 29-32. (in Chinese)
[9]Ma C H, Shang C, Jia Y S, Dong W Q, Li Y C, Hou L B. Induced resistance to Bipolaris maydis disease of maize C103 by culture filtrate of HMC toxin. Journal of Huazhong Agricultural University, 2008, 27(5): 590-596.
[10]商  闯, 贾银锁, 马春红, 董文琦, 李运朝, 崔四平, 侯立白. HMC毒素培养滤液对专化寄主玉米叶片诱导抗病性及相关酶的影响. 中国农业科学, 2008, 41(12): 4307-4313.
Shang C, Jia Y S, Ma C H, Dong W Q, Li Y C, Cui S P, Hou L B. The influence of filtrated culture fluid of HMC toxin on inducing disease-resistance and its related enzymes in specific host maize (Zea mays L.) leaves. Scientia Agricultura Sinica, 2008, 41(12): 4307-4313. (in Chinese)
[11]商  闯, 马春红, 翟彩霞, 李运朝, 董文琦, 崔四平, 侯立白. 低浓度玉米小斑病菌C小种毒素培养滤液诱导寄主抗性的研究. 华北农学报, 2008, 23(2): 198-204.
Shang C, Ma C H, Zhai C X, Li Y C, Dong W Q, Cui S P, Hou L B. Induced host disease by culture filtrate of Bipolaris maydis race C toxin cultivation in maize (Zea mays L.). Acta Agriculturae Boreali-Sinica, 2008, 23(2): 198-204. (in Chinese)
[12]商  闯, 贾银锁, 侯立白, 马春红. 玉米离体根冠细胞凋亡的荧光显微观察. 分子细胞生物学报, 2008, 41(4): 329-334.
Shang C, Jia Y S, Hou L B, Ma C H. Apoptosis in detached corn root cap cells under fluorescent microscope. Journal of Molecular Cell Biology, 2008, 41(4): 329-334. (in Chinese)
[13]Weinrauch Y, Zychlinsky A. The induction of apoptosis by bacterial pathogens. Annual Review of Microbiology, 1999, 53: 155-187.
[14]Gadjev I, Stone J M, Gechev T S. Programmed cell death in plants: new insights into redox regulation and the role of hydrogen peroxide. International Review of Cell and Molecular Biology, 2008, 270: 87-144.
[15]Greenberg J T. Programmed cell death: a way of life for plants. Proceedings of the National Academy of Science of United States of America, 1996, 93(22): 12094-12097.
[16]Wang H, Li J, Bostock R M, Gilchrist D G. Apoptosis: a functional paradigm for programmed plant cell death induced by a host-selective phytotoxin and invoked during development. The Plant Cell, 1996, 8(3): 375-391.
[17]夏启中, 吴家和, 张献龙. 与植物超敏反应(HR)相关的细胞编程性死亡. 华中农业大学学报, 2005, 24(1): 97-103.
Xia Q Z, Wu J H, Zhang X L. Review on hrsensitive response related PCD in plant. Journal of Huazhong Agricultural University, 2005, 24(1): 97-103. (in Chinese)
[18]Deng X Y, Li J W, Zhou Z Q, Fan H Y. Cell death in wheat roots induced by the powdery mildew fungus Blumeria graminis f. sp. tritici. Plant and Soil, 2010, 328(1/2): 45-55.
[19]Wang L K, Zhou Z Q, Song X F, Li J W. Evidence of ceased programmed cell death in metaphloem sieve elements in the developing caryopsis of Triticum aestivum L.. Protoplasma, 2008, 234(1-4): 87-96.
[20]He X, Kermode A R. Proteases associated with programmed cell death of megagametophyte cells after germination of white spruce (Picea glauca) seeds. Plant Molecular Biology, 2003, 52(4): 729-744.
[21]夏启中, 张明菊, 张献龙, 郭小平. 高浓度细胞分裂素诱导棉花悬浮细胞程序性死亡. 华中农业大学学报, 2005, 24(4): 334-338.
Xia Q Z, Zhang M J, Zhang X L, Guo X P. High concentration of cytokinin induces programmed cell death of cotton suspension cultures. Journal of Huazhong Agricultural University, 2005, 24(4): 334-338. (in Chinese)
[22]崔  洋, 刘克明, 魏建昆, 张为国, 欧阳光察. 玉米小斑病菌C小种毒素的分离、纯化及其植物病理反应. 植物病理学报, 1991,  21(3): 187-191.
Cui Y, Liu K M, Wei J K, Zhang W G, Ouyang G C. 1solation and purification of a C-toxin complex from Bipolaris maydis race C and its pathological reactions. Acta Phytopathologica Sinica, 1991, 21(3): 187-191. (in Chinese)
[23]吴  兴, 陈峥嵘, 张光健. AO/ EB双重染色法检测人骨肉瘤细胞凋亡. 肿瘤, 2004, 24(1): 88-89.
Wu X, Chen Z R, Zhang G J. The detection of apoptosis in human osteosarcoma cell with AO/EB double staining. Tumor, 2004, 24(1): 88-89. (in Chinese)
[24]董金皋, 韩建民, 李  竹. 玉米大斑病菌HT-毒素对玉米细胞膜透性和Vc氧化酶、PPO活性的影响. 玉米科学, 1997, 5(2): 77-80.
Dong J G, Han J M, Li Z. Influence of Ht-toxin produced by Helminthosporium turcicum on membrane leakage, Vc oxidenzyme and PPO activity. Maize Sciences, 1997, 5(2): 77-80. (in Chinese)
[25]郝丽梅, 王立安, 马春红, 闫芝芬, 王建斌. 致病真菌与植物寄主相互作用关系的研究进展. 河北农业科学, 2001, 5(2): 73-78.
Hao L M, Wang L A, Ma C H, Yan Z F, Wang J B. Research advances in interaction between pathogenic fungi and plant host. Journal of Hebei Agricultural Science, 2001, 5(2): 73-78. (in Chinese)
[26]郝丽梅. HMC毒素对玉米CMS与N细胞质线粒体结构和功能的影响[D]. 石家庄: 河北师范大学, 2002.
Hao L M. The effect of HMC-toxin to the structure and function of mitochondria in maize with CMS and normal cytoplasm[D]. Shi jiazhuang: Hebei Normal University, 2002. (in Chinese)
[27]章元寿, 王建新, 周明国, 刘经芬, 方中达. 棉花黄萎病菌毒素对棉花作用机制的探讨. 植物病理学报, 1991, 21(1): 49-52.
Zhang Y S, Wang J X, Zhou M G, Liu J F, Fang Z D. Preliminary studies on the mechanism of action with Vd-toxins from Verticillium dahliae Kleb. in cotton. Acta Phytopathologica Sinica, 1991, 21(1): 49-52. (in Chinese)
[28]Fan W W, Wang L A, Ma C H, Dong W Q, Li Y C, Liu Z H, Jia Y S, Geng J Y, Zhang X Y. The influence of the Verticillium dahliae Kleb infection on the anti-enzyme inside the body of the cotton with different root injured degree. Agricultural Sciences in China, 2007, 6(7): 816-824.
[29]马春红, 范尉尉, 董文琦, 李运朝, 崔四平, 贾银锁. 黄萎病菌毒素粗提液对棉花抗性酶的诱导. 中国农业科学, 2008, 41(12): 4314-4320.
Ma C H, Fan W W, Dong W Q, Li Y C, Cui S P, Jia Y S. Infection of Verticillium dahliae toxin filtrate inducing anti-enzyme in cotton (Gossypium hirsutum L.). Scientia Agricultura Sinica, 2008, 41(12): 4314-4320. (in Chinese)
[30]Curtis M J, Wolpert T J. The oat mitochondrial permeability transition and its implication in victorin binding and induced cell death. The Plant Journal, 2002, 29(3): 295-312.
[31]Ma C H, Zhai C X, Wang L A, Chen X, Li Y C, Guo X L, Cui S P, Li G M. Induced resistance by the toxin filtrate of Bipolaris maydis race T cultivation. Agricultural Sciences in China, 2006, 5(9): 678-684. (in Chinese)
[32]马春红, 翟彩霞, 郑秋玲, 董文琦, 李运朝, 崔四平, 范尉尉, 贾银锁. 玉米小斑病菌T小种毒素诱导对玉米叶片苯丙氨酸解氨酶活性的影响. 华中农业大学学报, 2010, 29(1): 21-25.
Ma C H, Zhai C X, Zheng Q L, Dong W Q, Li Y C, Cui S P, Fan W W, Jia Y S. Effect of inducement of HMT-toxin to PAL activity in maize leaves. Journal of Huazhong Agricultural University, 2010, 29(1): 21-25. (in Chinese)
[33]陈  颖, 杨  虹, 霍晨敏, 王立安. HMC毒素对同核异质玉米叶肉细胞原生质体的影响. 河北师范大学学报: 自然科学版, 2007, 31(5): 662-665.
Chen Y, Yang H, Huo C M, Wang L A. Effects of HMC toxin on protoplasts of Charrua male-sterility maize. Journal of Hebei Normal University: Natural Science Edition, 2007, 31(5): 662-665. (in Chinese)
[34]王立安, 马春红, 赵俊霞, 魏建昆. HMC-毒素对C型不育玉米根冠细胞原生质体及微丝分布的影响. 植物病理学报, 2003, 33(3)：225-228. 
Wang L A, Ma C H, Zhao J X, Wei J K. The effects of HMC-toxin on the protoplasts and their actin filaments distribution in detached root cap cells of Charrua cytoplasmic male sterility maize. Acta Phytopathologica Sinica, 2003, 33(3): 225-228. (in Chinese)
[35]马春红, 李秀丽, 董文琦, 李运朝, 崔四平, 贾银锁, 王立安. 玉米小斑病菌C小种毒素诱导玉米离体根冠细胞凋亡的检测. 华中农业大学学报, 2010, 29(2): 138-142.
Ma C H, Li X L, Dong W Q, Li Y C, Cui S P, Jia Y S, Wang L A. Detection of apoptosis in detached maize root cap cells induced by HMC-toxin. Journal of Huazhong Agricultural University, 2010, 29(2): 138-142. (in Chinese)
[36]Panda S K, Yamamoto Y, Kondo H, Matsumoto H. Mitochondrial alterations related to programmed cell death in tobacco cells under aluminium stress. Comptes Rendus Biologies, 2008, 331(8): 597-610.
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