利用SSR分子标记研究国内外黍稷地方品种和野生资源的遗传多样性

doi:10.3864/j.issn.0578-1752.2016.17.002

Abstract

Abstract: 【Objective】The objective of this study is to assess the genetic diversity of broomcorn millet accessions which collected from China and abroad.【Method】Five hundred pairs of SSR primers developed in the authors’ laboratory by high-throughput sequencing were used to identify polymorphisms in six representatives randomly selected from the total of accessions. A total of 63 primer pairs produced clear and reproducible polymorphic fragments among the six accessions and then were used to analyze the genetic diversity and relationship of 192 broomcorn millet landraces and wild accessions.【Result】A total of 161 alleles were detected with an average of 2.56 alleles per locus, and the mean Shannon-Weaver index (I), mean Nei and mean PIC were 0.6275, 0.3874 and 0.4855, respectively. The results indicated that there is a significant difference among the 10 populations of broomcorn millet resources in genetic diversity from diverse geographic origins. The variance range of effective alleles number is 1.2407 (South region) - 1.8846 (Inner Mongolia). In domestic populations, the rank of Shannon-Weaver index is Inner Mongolia Plateau＞Tohoku＞Loess Plateau＞Northwest＞southern regions, and the rank of foreign populations is the former Soviet Union＞Europe＞Mongolia＞India＞United States. The results of Nei’s genetic heterozygosity analysis showed that the minimum and maximum of observed (Ho) and expected heterozygosity (He) is 0.2372 from India and 0.3966 from Inner Mongolia as well as 0.3114 from the Unite State and 0.4622 from Inner Mongolia Plateau, respectively. The effective number of alleles (1.9285±0.5101), Shannon-Weaver index (0.6948±0.2852) and Nei gene diversity index (0.4373±0.1773) of the wild germplasm are much higher which in domestic and foreign accessions. For domestic population and alien population, the effective number of alleles (1.8145±0.4519) of domestic resources, Shannon-Weaver index (0.6657±0.2413), and Nei gene diversity index (0.412± 0.1574) of domestic accessions were higher than that in foreign resources (1.6862±0.4527, 0.5897±0.2469, 0.3652±0.1655). UPGMA cluster analysis showed that the 10 geographic populations could be clustered into three categories, the accessions from the Inner Mongolia Plateau, the Loess Plateau, northeast, northwest, Mongolia area were clustered as one group, the former Soviet Union, the United States, India, Europe together as one group, and southern region of China clustered as one independent group. The wild millet (34) which is from Qiqihaer is separated from others at 0.37, the wild millet from Gansu (19) was divided into an independent individual at 0.34, indicating that there are significant genetic variances between the two wild accessions and others. In general, genetic division of population is not significant for 192 domestic and foreign accessions, and there have material interpenetration among different groups.【Conclusion】The Inner Mongolian Plateau, northeast area and the Loess Plateau with the most abundant genetic diversity is the most complex area of genetic relationship, which further confirms that China is the origin center of Panicum miliaceum.

Key words: Panicum miliaceum L, landraces, wild species, SSR markers, genetic diversity

LIAN Shuai, LU Ping, QIAO Zhi-jun, ZHANG Qi, ZHANG Qian, LIU Min-xuan, WANG Rui-yun. Genetic Diversity in Broomcorn Millet (Panicum miliaceum L.) from China and Abroad by Using SSR Markers[J].Scientia Agricultura Sinica, 2016, 49(17): 3264-3275.

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Genetic Diversity in Broomcorn Millet (Panicum miliaceum L.) from China and Abroad by Using SSR Markers

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