Abstract:

【Objective】 The present study was aimed to develop a RIL (recombinant inbred line) population, construct its genetic map and analyze quantitative trait loci (QTL) associated with important agronomic traits in wheat. This work will accelerate gene discovery and marker-assistant breeding of wheat. 【Method】 A RIL population was derived from the cross of Yanzhan1 and Zaosui 30 by single seed descent approach. SSR (simple sequence repeat), DarT (diversity arrays technology), ISBP (insertion site-based polymorphism) and functional makers associated with days to heading (DH) and plant height (PH) were used to construct genetic map. QTLs related to DH, PH, thousand grain weight (TGW), grain number per spike (GNS), spike length (SL) and spikelet numbers (SPI) in different environments were detected by using the composite interval mapping (CIM). 【Result】 A set of 219 F7 RILs was derived. A genetic linkage map with 481 molecular markers covering the whole wheat genome was constructed. A total of 26 QTLs for the related traits located on 12 chromosomes were detected, among which 9 QTLs were detected in at least two environments. Three QTL clusters were revealed including one cluster for PH and TGW located on dwarf gene Rht2 region on 4DS, one cluster for DH, GNS and SPI located in region Vrn-D1-WMS212 of chromosome 5D and the other cluster for DH, SPI, PH, and SL located in the interval of wPt4230 and wPt4814 on chromosome 7B. 【Conclusion】 The genetic map is favorable to analyze important agronomic traits. Dwarfing gene Rht2 and vernalization gene Vrn-D1, two developmental related genes, are related to some important agronomic traits. There might be a novel gene concerning plant development on chromosome 7B.

Key words: wheat, molecular marker, QTL