利用SSH技术鉴定黄瓜胚胎发育相关基因

doi:10.3864/j.issn.0578-1752.2010.11.012

Abstract

Abstract:

【Objective】 The aim of this experiment is to investigate the embryo development-related genes in cucumber, and study its molecular mechanism.【Method】 A subtracted cDNA library was constructed by using Suppression Subtractive Hybridzation (SSH). 【Result】 A total of 97 positive clones were screened using reverse Northern blot hybridization. After the removal of duplication and sequencing of low-quality sequences, 93 UniESTs were identified, including 86 singletons and 7 contigs. The 93 unique ESTs were used to BLAST in the GenBank non-redundant database. There were 83 ESTs were found matching homologous sequences, 10 ESTs homologous sequence were not found, presumably may be new genes. 【Conclusion】 Many of these ESTs were highly homologous with the heat shock proteins of Arabidopsis, rice and corn. Application of the GeneOntology functional classification, cell defense and metabolic processes in early embryos accounted for the main location. Cell defense and anti-osmotic stress function is very important in late embryos.

Key words: cucumber, SSH, embryonic development, cDNA library

SHENG Hui,QIN Zhi-wei,ZHOU Xiu-yan,WU Tao
.

Identification of Embryo Development-Related Genes in Cucumber Using SSH

[J].Scientia Agricultura Sinica, 2010, 43(11): 2292-2299 .

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.chinaagrisci.com/EN/10.3864/j.issn.0578-1752.2010.11.012

https://www.chinaagrisci.com/EN/Y2010/V43/I11/2292

References

Related Articles 15

[1]	LI QingLin,ZHANG WenTao,XU Hui,SUN JingJing. Metabolites Changes of Cucumber Xylem and Phloem Sap Under Low Phosphorus Stress [J]. Scientia Agricultura Sinica, 2022, 55(8): 1617-1629.
[2]	KANG Chen,ZHAO XueFang,LI YaDong,TIAN ZheJuan,WANG Peng,WU ZhiMing. Genome-Wide Identification and Analysis of CC-NBS-LRR Family in Response to Downy Mildew and Powdery Mildew in Cucumis sativus [J]. Scientia Agricultura Sinica, 2022, 55(19): 3751-3766.
[3]	CHEN Xi,LIU YingJie,DONG YongHao,LIU JinYan,LI Wei,XU PengJun,ZANG Yun,REN GuangWei. Effects of CMV-Infected Tobacco on the Performance, Feeding and Host Selection Behavior of Myzus persicae [J]. Scientia Agricultura Sinica, 2021, 54(8): 1673-1683.
[4]	WANG JunZheng,ZHANG Qi,GAO ZiXing,MA XueQiang,QU Feng,HU XiaoHui. Effects of Two Microbial Agents on Yield, Quality and Rhizosphere Environment of Autumn Cucumber Cultured in Organic Substrate [J]. Scientia Agricultura Sinica, 2021, 54(14): 3077-3087.
[5]	ZHANG Wei,WANG ShiYin,GAO Li,YANG LiWei,DENG ShuangYi,LIU XiaoNa,SHI GuoQing,GAN ShangQuan. Investigation of miR-486 Target Genes in Skeletal Muscle of Bashbay Sheep in Different Development Periods [J]. Scientia Agricultura Sinica, 2021, 54(14): 3134-3148.
[6]	LI ZhengGang,NONG Yuan,TANG YaFei,SHE XiaoMan,YU Lin,LAN GuoBing,DENG MingGuang,HE ZiFu. Molecular Characteristic and Pathogenicity Analyses of Cucumber green mottle mosaic virus (CGMMV) Infecting Bottle Gourd in Lianzhou, Guangdong [J]. Scientia Agricultura Sinica, 2020, 53(5): 955-964.
[7]	ZHOU Qi,LIU XiaoPing,BO KaiLiang,MIAO Han,DONG ShaoYun,GU XingFang,ZHANG ShengPing. Cloning and Analysis of Folate Synthesis Key Genes in Cucumber [J]. Scientia Agricultura Sinica, 2020, 53(18): 3764-3776.
[8]	HeXu CAI,KaiLiang BO,Qi ZHOU,Han MIAO,ShaoYun DONG,XingFang GU,ShengPing ZHANG. GWAS Analysis of Hypocotyl Length and Candidate Gene Mining in Cucumber Seedlings [J]. Scientia Agricultura Sinica, 2020, 53(1): 122-132.
[9]	Jian PAN,HaiFan WEN,HuanLe HE,HongLi LIAN,Gang WANG,JunSong PAN,Run CAI. Genome-Wide Identification of Cucumber ERF Gene Family and Expression Analysis in Female Bud Differentiation [J]. Scientia Agricultura Sinica, 2020, 53(1): 133-147.
[10]	WeiYuan SONG,Yu HOU,JianYu ZHAO,XiaoFeng LIU,XiaoLan ZHANG. Cloning and Functional Analysis of CsRPL1/2 in Cucumber [J]. Scientia Agricultura Sinica, 2020, 53(1): 148-159.
[11]	ZhiHong NIU,XiaoFei SONG,XiaoLi LI,XiaoYu GUO,ShuQiang HE,LuanJingZhi HE,ZhiHong FENG,ChengZhen SUN,LiYing YAN. Inheritance and QTL Mapping for Parthenocarpy in Cucumber [J]. Scientia Agricultura Sinica, 2020, 53(1): 160-171.
[12]	Fei QI,Shu LIN,MengFei SONG,MengRu ZHANG,ShuYan CHEN,NaiXin ZHANG,JinFeng CHEN,QunFeng LOU. Screening and Identification of Cucumber Mutant Resistant to Powdery Mildew [J]. Scientia Agricultura Sinica, 2020, 53(1): 172-182.
[13]	CHUAI HongYun,SHI YanXia,CHAI ALi,YANG Jie,XIE XueWen,LI BaoJu. Development of 10% Diethofencarb·Procymidone Micropowder and Its Control Efficacy to Cucumber Corynespora Leaf Spot [J]. Scientia Agricultura Sinica, 2019, 52(6): 1009-1020.
[14]	ZhiPing LIU,XuePing WU,RuoNan LI,FengJun ZHENG,MengNi ZHANG,ShengPing LI,XiaoJun SONG. Effect of Applying Chicken Manure and Phosphate Fertilizer on Soil Phosphorus Under Drip Irrigation in Greenhouse [J]. Scientia Agricultura Sinica, 2019, 52(20): 3637-3647.
[15]	RuoNan LI,ShaoWen HUANG,JianShuo SHI,LiYing WANG,JiWei TANG,HuaiZhi ZHANG,Shuo YUAN,FengZhi ZHAI,YanLi REN,Li GUO. Optimization Management of Water and Fertilization for Winter-Spring Cucumber Under Greenhouse Drip Irrigation Condition [J]. Scientia Agricultura Sinica, 2019, 52(20): 3648-3660.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

Comments

Recommended 0

No Suggested Reading articles found!