Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (10): 3397-3404 .doi: 10.3864/j.issn.0578-1752.2009.10.003

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Genetic Diversity of the Founder Parent Orofen and Its Progenies Revealed by SSR Markers

LI Xiao-jun, XU Xin, LIU Wei-hua, LI Xiu-quan, LI Li-hui
  

  1. (中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程)
  • Received:2009-01-04 Revised:2009-04-07 Online:2009-10-10 Published:2009-10-10
  • Contact: LI Li-hui

Abstract:

【Objective】 The main objective is to uncover the genetic contribution of founder parent to its offspring through tracking chromosomal regions of Orofen in its progeny. 【Method】 Orofen and its 23 representative progenies were analyzed using morphological traits in five environments and microsatellite markers. 【Result】 The frequencies of the alleles which were amplified in Orofen but absent in other 4 parental accessions (Jinan 4, Beijing 8, 54405 and Heine Hvede) at 43 SSR loci were higher than the theoretical ratio 0.38 in the first generation; the frequencies of the alleles which were amplified in Orofen but absent in other 6 parental accessions (Jinan 4, Beijing 8, 54405, Heine Hvede, Lovrin 10 and Lovrin 18) at 41 SSR loci were higher than the theoretical ratio 0.18 in the second generation; at twenty-one of the found loci above, the unique alleles amplified only in Orofen have been selected continuously in the both generations. Association mapping indicated that the alleles according to Orofen which occurred in most progenies at the loci Xwmc710, Xbarc235 and Xbarc252 were associated with higher grain number and yield when compared with the remaining amplified alleles at the same loci. 【Conclusion】 It was speculated that the genomic region detected and associated traits played an important role for the improvement of wheat in China and could be acted as the emphases for further study.

Key words: wheat, founder parent, chromosomal loci, selection, association mapping

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