番木瓜性别连锁的AFLP及其SCAR标记的建立

doi:10.3864/j.issn.0578-1752.2009.03.027

Scientia Agricultura Sinica ›› 2009, Vol. 42 ›› Issue (3): 967-973 .doi: 10.3864/j.issn.0578-1752.2009.03.027

• HORTICULTURE • Previous Articles Next Articles

Development of Sex Linked AFLP-Derived SCAR Markers in Carica papaya

中国农业大学农业生物技术国家重点实验室/农业部作物基因组学与遗传改良重点开放实验室/北京市作物遗传改良重点实验室/教育部作物杂种优势研究与利用重点实验室

Received:2008-03-25 Revised:2008-05-09 Online:2009-03-10 Published:2009-03-10
Contact: LIU Zhi-yong

Abstract

Abstract:

【Objective】 Papaya (Carica papaya L.), native to tropical America, is a widely distributed fruit crop throughout the tropical and warmer subtropical regions of the world. Papaya is a polygamous plant species with three sex forms: female, male, and hermaphrodite. Sex determination in the young seedling stage is very important for papaya plantation. 【Method】 By cloning sex-linked AFLP markers, 31 Carica papaya sex linked SCAR markers were developed and validated at different sex types of papaya plants and segregation population. 【Result】 Sixteen SCAR markers were found to be polymorphic between different sex types and were co-segregated with male and hermaphrodite sex, and therefore can be used to discriminate the female and hermaphrodite or male tree, while the other 15 SCAR markers were monomorphism yielding same size DNA fragments at three sex types. 【Conclusion】These AFLP-derived SCAR markers can be used as diagnostic tools for molecular discrimination of different papaya sex types at seedling stage and as probes to screen papaya BAC library to construct physical map around the papaya sex determination gene.

Key words: Carica papaya L., sex, AFLP, SCAR

. Development of Sex Linked AFLP-Derived SCAR Markers in Carica papaya
[J].Scientia Agricultura Sinica, 2009, 42(3): 967-973 .

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