Scientia Agricultura Sinica ›› 2019, Vol. 52 ›› Issue (5): 949-954.doi: 10.3864/j.issn.0578-1752.2019.05.016

• RESEARCH NOTES • Previous Articles    

Genetic Analysis of Sex-Linked Plumage Color Traits of Goose

YU JinCheng1,LI Zhe1,YU Ning1,LIU Kuang2,ZHAO Hui1()   

  1. 1 Liaoning Academy of Agricultural Sciences, Shenyang 110161
    2 Haicheng Zhengfeng Animal Husbandry Company, Haicheng 114222, Liaoning
  • Received:2018-07-25 Accepted:2019-01-16 Online:2019-03-01 Published:2019-03-12
  • Contact: Hui ZHAO E-mail:zhaohui_sy@126.com

Abstract:

【Objective】The Huoyan breed of geese is a famous white goose breed in China, 1-day-old gosling of the Huoyan goose are yellow or light yellow. In the offspring of the free mating group, 20% of 1-day-old goslings were found to have a light brown variation during Huoyan goose breeding process, and the ratio of male to female is obviously different (the ratio of male to female is close to 1﹕3), it is speculated that there is a sex-linked inheritance tendency in goose plumage color trait. Using the light brown male geese and yellow female geese as the parents, the hybrid experiment was used to test whether the phenotype of the gosling downy feathers conformed to the Mendelian inheritance law, so as to understand the genetic mechanism of goose plumage color trait. The study on the inheritance and mechanism of feather color traits of goose is of great significance for the breeding of new breeds or strains of goose and the goose specialized line cultivation of auto-sexing by feather color. 【Method】40 males and 200 females of Huoyan geese were chosen to generate the G1 generation of Huoyan goose pure line by random mating, and plumage color appearance and segregation ratio in G1 were observed. 4 light brown males and 20 white feather color females of Huoyan geese were chosen to generate the F1 generation, and plumage color appearance and segregation ratio in F1 were observed. 【Result】① In the random mating G1 generation of the Huoyan goose, the proportion of light brown downy feather trait individuals was 19.5%. Among them, the ratio of light brown individuals between male and female chicks is relatively large, the proportion of light brown in male chicks is about 10%, and 30% of females are light brown. According to Hardy-Weinberg’s law, the allele frequency of the color traits of the downy feather is calculated as follows: The ratio of light brown individuals in the female goslings is the frequency of the light brown trait alleles in the Huoyan goose population, in this experiment, 29.8% of the female are light brown, so the frequency of the light brown trait allele is about 0.3. At the same time, the ratio of the light brown individual in the male goslings can also calculate the frequency of the light brown allele, in this case, 10% of the male are light brown, so the frequency of the light brown trait allele is about 0.3. Correspondingly, the frequency of the yellow downy feather trait allele is about 0.7. ②Among the F1 populations, 81 were yellow feathers, accounting for 50%; 79 were light brown, accounting for 50%. And the yellow feathers are all male, and the light brown feathers are all female. The results showed that the light brown traits were associated with sex-linked inheritance. It has been confirmed that silver feather (S*S) and imperfect albinism (S*AL) are controlled by a pair of alleles located on the Z chromosome, and the imperfect albinism gene AL is recessive to the silver feather gene S. Therefore, based on the results of the hybridization test, it can be concluded that the light brown and yellow are the results determined by the multiple allele of one locus, and the light brown color is recessive inheritance for the yellow trait.【Conclusion】In this experiment, the separation of 1-day-old goslings feather phenotypes was analyzed by downy feather color matching hybridization and random mating test. The results showed that the light brown downy feather color trait of Huoyan goose exhibits recessive heredity to the yellow, and demonstrates sex-linked inheritance. The downy feather color of the Huoyan goose is mainly controlled by the Z chromosome silver feather locus. The variation site of the allele controlling the locus is associated with the corresponding appearance.

Key words: plumage color trait, recessive inheritance, sex-linked inheritance, Huoyan goose

Table 1

The amount of plumage color trait in G1 generation of Huoyan goose random-mating populations"

群体
Populations
黄色(只)
Yellow plumage
浅褐色(只)
Light brown plumage
浅褐色百分率
Percentage of geese with light brown plumage
实际比值Real ratio
黄色﹕浅褐色
Yellow﹕Light brown
基因频率
Gene frequency
Male
Female
Male
Female
Male♂+ Female♀ Male
Female
S AL
豁眼鹅G1
Huoyan goose G1 generation
160 115 18 49 19.5% 9:1 2:1 0.7 0.3

Table 2

The comparison of reality and theory amount of plumage color trait of F1 generation"

世代
Generations
黄色(只)
Yellow plumage
浅褐色(只)
Light brown plumage
实际比值Real ratio
黄色﹕浅褐色
Yellow﹕Light brown
理论比值Theoretical ratio
黄色﹕浅褐色
Yellow﹕Light brown
卡方检验
Chisquare
test (χ2 value)
Male
Female
Male
Female
Male
Female
Male
Female
Male
Female
F1 81 0 0 79 1﹕0 0﹕1 1﹕0 0﹕1 * *
[1] PEARL R, SURFACE F M . On the inheritance of the barred color pattern in poultry. Archiv für Entwicklungsmechanik der Organismen, 1910,30(1):45-61.
doi: 10.1007/BF02263780
[2] KNOX C W. The genetics of plumage colour in poultry. Iowa Agricultral Experiment Station, 1926, Bulletin, No. 105.
[3] NORDSKOG A W . Inheritance of shank and plumage color in the Fayoumi breed. Poultry Science, 1970, 49(6):1735-1737.
doi: 10.3382/ps.0491735
[4] 王存芳, 李宁, 吴常信 . 基于F2群体的藏鸡羽色、胫色性状的遗传分析. 遗传, 2006,28(7):810-814.
doi: 10.3321/j.issn:0253-9772.2006.07.008
WANG C F, LI N, WU C X . Genetic analysis of feather color and shank color trait based on F2 resource population in Tibetan chicken. Hereditas, 2006,28(7):810-814. (in Chinese)
doi: 10.3321/j.issn:0253-9772.2006.07.008
[5] JAAP R G . Alleles of the mallard plumage pattern in ducks. Genetics, 1934, 19(4):310-322.
doi: 10.1007/BF02982235 pmid: 17246725
[6] GONG Y, YANG Q, LI S, FENG Y, GAO C, TU G, PENG X . Grey plumage coloration in the duck is genetically determined by the alleles on two different, interacting loci. Animal Genetics, 2010,41:105-108.
doi: 10.1111/j.1365-2052.2009.01967.x pmid: 19814756
[7] LI S J, WANG C, YU W H, ZHAO S H, GONG Y Z . Identification of genes related to white and black plumage formation by RNA-Seq from white and black feather bulbs in ducks. PLoS ONE, 2012,7(5):e36592.
doi: 10.1371/journal.pone.0036592 pmid: 3352928
[8] LIN R L, CHEN H P, ROUVIER R, POIVEY J P . Selection and crossbreeding in relation to plumage color inheritance in three chinese egg type duck breeds (Anas Platyrhynchos). Asian-Australasian Journal of Animal Sciences, 2014,27(8):1069-1074.
doi: 10.5713/ajas.2013.13289 pmid: 4109861
[9] ZHOU Z K, LI M, CHENG H, FAN W L, YUAN Z R, GAO Q, XU Y X, GUO Z B, ZHANG Y S, HU J, LIU H H, LIU D P, CHEN W H, ZHENG Z Q, JIANG Y, WEN Z G, LIU Y M, CHEN H, XIE M, ZHANG Q, HUANG W, WANG W, HOU S S, JIANG Y . An intercross population study reveals genes associated with body size and plumage color in ducks. Nature Communications, 2018, 9(1):2648.
doi: 10.1038/s41467-018-04868-4 pmid: 30018292
[10] LAUBER J K . Sex-linked albinism in the Japanese quail. Science, 1964,146(3646):948-950.
doi: 10.1126/science.146.3646.948-a pmid: 14199723
[11] TRUAX R E, JOHNSON W A . Genetics of plumage color mutants in Japanese quail. Poultry Science, 1979, 58(1):1-9.
doi: 10.1093/fs/17.4.399
[12] MINVIELLE F, ITO S, INOUE-MURAYAMA M, MIZUTANI M, WAKASUGI N . Brief communication. Genetic analyses of plumage color mutations on the Z chromosome of Japanese quail.Journal of Heredity, 2000, 91(6):499-501.
doi: 10.1093/jhered/91.6.499 pmid: 11218091
[13] STURTEVANT A H . An experiment dealing with sex-linkage in fowls. Journal of Experimental Zoology, 1912, 12:499-518.
doi: 10.1002/jez.1400120404
[14] 陈国宏, 王继文, 何大乾, 王志跃, 王宝维 .中国养鹅学. 北京: 中国农业出版社, 2013: 43-44.
CHEN G H, WANG J W, HE D Q, WANG Z Y, WANG B W. China Goose Husbandry Science. Beijing: China Agricultural Press, 2013: 43-44. (in Chinese)
[15] WERRET W F, CANDY A J KING J O L,SHEPPARD P M,. Semi-albino: a third sex-linked allelomorph of silver and gold in the fowl. Nature, 1959,184:480.
doi: 10.1038/184480a0 pmid: 13844061
[16] COLE R K, JEFFERS T K . Allelism of silver, gold, and imperfect albinism in the fowl. Nature, 1963,200:1238-1239.
doi: 10.1038/2001238a0
[17] MORGAN T H, GOODALE H D . Sex-linked inheritance in poultry. Annals of the New York Academy of Sciences, 1912,22(1):113-133.
doi: 10.1002/jez.1400130102
[18] SERBROVSKY A S . Crossing-over involving three sex-linked genes in Chickens. American Naturalist, 1922,56:571-572.
doi: 10.1086/279898
[19] WARREN D C . Sex-linked characters of poultry. Genetics, 1928, 13(5):421-433.
doi: 10.1007/BF02983144 pmid: 17246558
[20] MAW A J G . The inheritance of skeletal dimensions in the domestic fowl. Madison: University of Wisconsin, 1935.
[21] 庞有志, 赵淑娟 . 鹌鹑羽色遗传的研究及应用. 遗传, 2003,25(4):450-454.
doi: 10.3321/j.issn:0253-9772.2003.04.018
PANG Y Z, ZHAO S J . The study and use on genetics of plumage color in quail. Hereditas, 2003 25(4):450-454. (in Chinese)
doi: 10.3321/j.issn:0253-9772.2003.04.018
[22] 于金成, 李喆, 于宁, 赵辉 . 基于F2群体的豁眼鹅豁眼性状遗传分析. 中国农业科学, 2016,49(19):3845-3851.
doi: 10.3864/j.issn.0578-1752.2016.19.015
YU J C, LI Z, YU N, ZHAO H . Genetic analysis of hoyan trait based on F2 resource population in Huoyan Goose. Scientia Agricultura Sinica, 2016 49(19):3845-3851. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2016.19.015
[23] JEROME F N . Color inheritance in geese and its application to goose breeding. Poultry Science, 1953,32(1):159-165.
doi: 10.3382/ps.0320159
[24] JEROME F N . Color inheritance of geese: Study 2. Poultry Science, 1954,33(3):525-528.
doi: 10.3382/ps.0330525
[25] 侯水生 .我国肉鹅产业发展应重视的问题与建议. 水禽世界,2014(03):6-9.
doi: 10.3969/j.issn.1673-1085.2014.03.002
HOU S S .Problems and suggestions for the development of meat goose industry in China.Waterfowl World, 2014(03):6-9. (in Chinese)
doi: 10.3969/j.issn.1673-1085.2014.03.002
[26] 何艺平, 许继国, 谢袖娟, 詹惠娜 . 鸡羽色性状基因座研究进展. 广东农业科学, 2014,41(9): 129-134+237.
doi: 10.3969/j.issn.1004-874X.2014.09.030
HE Y P, XU J G, XIE X J, ZHAN H N . Research progress of feather color loci in Gallus gallus domestica. Agricultural Science of Guangdong, 2014,41(9): 129-134+237. (in Chinese)
doi: 10.3969/j.issn.1004-874X.2014.09.030
[27] BRUMBAUGH J A, BARGAR T W, WILLIAM O . A “new” allele at the C pigment locus in the fowl. Journal of Heredity, 1983, 74(5):331-336.
doi: 10.1093/oxfordjournals.jhered.a109804
[28] KERJE S, SHARMA P, GUNNARSSON U, KIM H, BAGCHI S, FEDRIKSSON R, SCHU K, JENSEN P, VON HEIJNE G, OKIMOTO R, ANDERSSON L . The dominant white, dun and smoky color variants in chicken are associated with insertion/ deletion polymorphisms in the PMEL17 gene. Genetics, 2004, 168(3):1507-1518.
doi: 10.1534/genetics.104.027995 pmid: 15579702
[29] CHANG C M, COVILLE J L, COQERELLE G, GOURICHON D, OULUMOUDEN A, TIXIER-BOICHARD M . Complete association between a retroviral insertion in the tyrosinase gene and the recessive white mutation in chickens. BMC Genomics, 2006,7:19.
doi: 10.1186/1471-2164-7-19 pmid: 1373650
[30] GUNNARSSON U, HELLSTRON A R, TIXIER-BOICHARD M, MINVIELLE F BED'HOM B,ITO S,JENSE P,RATTINK A,VEREIJKEN A,ANDERSSON L,. Mutations in SLC45A2 cause plumage color variation in chicken and Japanese quail. Genetics, 2007,175(2):867-877.
doi: 10.1534/genetics.106.063107 pmid: 17151254
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[2] YU Jin-cheng, LI Zhe, YU Ning, ZHAO Hui. Genetic Analysis of Huoyan Trait Based on F2 Resource Population in Huoyan Goose [J]. Scientia Agricultura Sinica, 2016, 49(19): 3845-3851.
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