Abstract: 【Objective】The objective of the research were analysis the Genetic diversity of Wheat Leaf Rust Resistance Near-isogenic Lines in Thatcher Background, and investigating the feasibility of SRAP marker applied on study of Wheat Leaf rust Resistance Genes marker and clone. 【Method】Total 23 wheat leaf rust resistance near-isogenic lines and Thatcher were carried out using SRAP(sequence-related amplified polymorphism)markers. 【Result】41of 128 SRAP primer combinations identified a total of 537 reproducible fragments. Among them, 49.5% were polymorphic, and 115 of them were specific bands (10.6%). Between 6 and 41 fragments were amplified per primer combination, with an average of 13.1 bands. The number of polymorphic fragments for each primer combination varied from 1 to 13, with an average of 2.8. A cluster analysis was performed using NTSYSpc Version 2.10e. Cluster analysis revealed 23 wheat leaf resistance genes and Thatcher into two clusters of A and B with the similarity coefficient of 0.72 . 96% of them were clustered into cluster B, which was divided into two groups,ⅠandⅡ. 95% of the near isogenic lines in cluster B fell into group Ⅱ. Group Ⅱ included four small groups with 0.785 similarity coefficient. 【Result】It was concluded that the 23 wheat leaf rust resistance near isogenic lines have more differences in molecular level. All these results showed SRAP marker is simple, economic and effective,it could be widely used in study of Wheat Leaf Rust resistance genes.

Key words: Wheat Leaf rust Resistance Genes, NILs, SRAP