Scientia Agricultura Sinica ›› 2013, Vol. 46 ›› Issue (10): 1974-1983.doi: 10.3864/j.issn.0578-1752.2013.10.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Establishment of Molecular Identity for Kenaf Germplasm Using SRAP Marker

 LIU  Qian, DAI  Zhi-Gang, CHEN  Ji-Quan, WEN  Lan, GONG  You-Cai, SU  Jian-Guang   

  1. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205
  • Received:2013-01-04 Online:2013-05-15 Published:2013-03-26

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Abstract: 【Objective】One hundred and twenty-seven accessions of kenaf gemplasm collected from different countries and areas, which contain cultivated, wild and related species, were selected and SRAP markers were used to establish molecular identity of kenaf germplasm.【Method】 Sequence- related amplified polymorphism(SRAP) marker was used for analyzing genetic relationships of the 127 different type accessions. Nei's similarity coefficients were generated and dendrogram was constructed using UMPGA by NTSYS-PC program.【Result】Three hundred and eighty-three bands were amplified by 40 SRAP primers, of which 375 were polymorphic and PPB was 97.9%. The cluster analysis based on UPGMA showed that the 127 accessions of kenaf germplasm were divided into four major groups at similarity coefficient of 0.70. All of the accessions could be effectively distinguished by specific bands and different primer combinations. The specific molecular identity for 127 accessions of kenaf germplasm were established with 99.99% probability of confidence.【Conclusion】A set of unique molecular identity was established for 127 kenaf germplasm based on 36 bands which were amplified by 15 core primer combinations.

Key words: kenaf germplasm , SRAP marker , DNA fingerprint , molecular identity

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