Scientia Agricultura Sinica ›› 2011, Vol. 44 ›› Issue (12): 2422-2430 .doi: 10.3864/j.issn.0578-1752.2011.12.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Construction of a Molecular Linkage Map Using SRAP and Mapping of Three Qualitative Traits in Corchorus olitorius L.

CHEN Hui; CHEN Mei-xia; TAO Ai-fen; ZHANG Guang-qing; XU Jian-tang; QI Jian-min; FANG Ping-ping
  

  1. 福建农林大学作物遗传育种综合利用教育部重点实验室
  • Received:2010-11-03 Revised:2010-11-26 Online:2011-06-15 Published:2011-06-15

Abstract:

【Objective】Jute (Corchorus olitorius L.) is one of the most economically important crops for fiber production. The present study was aimed to construct a molecular linkage map of jute and locate important qualitative traits, which will provide essential tools for investigating genomic structure and evolution, facilitating QTL mapping, marker-assisted selection and map-based cloning of important gene. 【Method】In this study, a population of F2 generation including 187 individual plants was derived from the cross between Corchorus aestuans L. (wild species) and wild leaf jute (cultivated species). A total of 513 sequence-related amplified polymorphisms (SRAP) primer pairs were used in constructing the molecular genetic linkage map and mapping 3 qualitative traits including color of stipule, color of stipe, and color of leaf margin.【Result】This genetic linkage map containing 10 linkage groups (LGs) was constructed using 122 SRAP loci and these 3 morphological markers, the map covered 2 231.9 cM with the average marker interval of 17.86 cM, every linkage group included 2-38 markers.【Conclusion】All the markers are distributed evenly among the linkage groups without clustering of loci. SRAP markers may be a better type of markers in map construction.

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