Scientia Agricultura Sinica ›› 2008, Vol. 41 ›› Issue (3): 636-642 .doi: 10.3864/j.issn.0578-1752.2008.03.002

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Isolation and chromosomal localization of cytokinin oxidase/dehydrogenase gene (TaCKX5) in wheat

  

  1. 沈阳农业大学农学院
  • Received:2007-01-05 Revised:1900-01-01 Online:2008-03-10 Published:2008-03-10

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Abstract: 【Objective】Genetic manipulation of CKX activity holds the promise of improving agricultural traits in crops.【Method】and【Result】In this study, a strategy of homologous cloning combined with BAC library screening was used to isolate TaCKX5 gene from wheat. Sequence analysis of TaCKX5 revealed that TaCKX5 contains a FAD-binding domain which is well conserved in the N-terminal halves of all known CKX sequences. Using TargetP program, TaCKX5 was predicted to be a secretion pathway protein, and one N-glycosylation site (NFTE) was also found with NetNGly program, implied that TaCKX5 protein may be modified posttranslationally by glycosylation. Using nulli-tetrasomic stocks, TaCKX5 was located on wheat homoeologous group 3 chromosomes.【Conclusion】TaCKX5 was very closely related to its orthologous CKX gene (OsCKX5) in rice, so they might have similar characters and functions.

Key words: Wheat, Cytokinin Oxidase/dehydrogenase, Cloning, Chromosomal Localization.

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