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2025 Vol. 24 No. 2 Previous Issue   

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Review
Crop Science
Horticulture
Plant Protection
Animal Science · Veterinary Medicine
Agro-ecosystem & Environment
Letter


  Cover illustration


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Cover illustration


Modern commercial tomato fruit products have substantially less flavor than those from heirloom varieties. A better understanding of the key genes that regulate flavor in tomato would help to restore the quality of fruit for commercial production worldwide. CONSTANS (CO) and CONSTANS-LIKE (COL) transcription factors are known to regulate a series of cellular processes, including the transition from vegetative growth to flower development in plants. However, their role in regulating the fruit chlorophyll content is poorly understood. In this study, SlCOL1, the tomato (Solanum lycopersicum) ortholog of Arabidopsis CONSTANS, was shown to play key roles in controlling fruit chlorophyll. An analysis of protein–protein interactions indicated that SlCOL1 regulates chlorophyll accumulation in tomato fruit by interacting with SlGLK2, and SlCOL1 and SlBBX24 may control it by promoting the stability of SlGLK2. Meanwhile, we also found GLK2 is required for the function of SlCOL1 in regulating chlorophyll content. These results shed new light on the mechanisms used by COL1 and GLK2 to regulate fruit development and chlorophyll accumulation in tomato. Thus, the fine tuning of SlCOL1 expression could potentially improve the chlorophyll content of tomato fruit. The cover photo was provided by Prof. Junhong Zhang, National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, China. For more details, please see pages 536–545.


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Review

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Fine control of growth and thermotolerance in the plant response to heat stress Yulong Zhao, Song Liu, Kaifeng Yang, Xiuli Hu, Haifang Jiang 2025, 24(2): 409-428.  DOI: 10.1016/j.jia.2024.03.028 Abstract ( )   PDF in ScienceDirect   Global warming impacts plant growth and development, which in turn threatens food security.  Plants can clearly respond to warm-temperature (such as by thermomorphogenesis) and high-temperature stresses.  At the molecular level, many small molecules play crucial roles in balancing growth and defense, and stable high yields can be achieved by fine-tuning the responses to external stimuli.  Therefore, it is essential to understand the molecular mechanisms underlying plant growth in response to heat stress and how plants can adjust their biological processes to survive heat stress conditions.  In this review, we summarize the heat-responsive genetic networks in plants and crop plants based on recent studies.  We focus on how plants sense the elevated temperatures and initiate the cellular and metabolic responses that allow them to adapt to the adverse growing conditions.  We also describe the trade-off between plant growth and responses to heat stress.  Specifically, we address the regulatory network of plant responses to heat stress, which will facilitate the discovery of novel thermotolerance genes and provide new opportunities for agricultural applications.

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Advances on ALV-J in China over the past two decades Wenrui Fan, Yuntong Chen, Mengmeng Yu, Yongzhen Liu, Yulong Gao 2025, 24(2): 429-440.  DOI: 10.1016/j.jia.2024.05.009 Abstract ( )   PDF in ScienceDirect   Avian leukosis is an important tumorigenic disease caused by the avian leukosis virus (ALV) in poultry.  ALVs belong to the retroviral family and are classified into 11 subgroups (ALV-A to ALV-K).  Among them, ALV-J was first introduced into China in 1999, spreading widely and evolving from infecting meat-type chickens to layer chickens and Chinese local chickens.  ALV-J typically induces myeloid leukosis in infected chickens, but also induces a high proportion of hemangiomas in infected layer chickens, posing a serious threat to poultry breeds in China.  As a retrovirus, the genome of ALV-J has undergone significant mutations, which may be related to the expansion of the infection host range and increased pathogenicity of ALV-J.  Over the last two decades, the introduction and spread of ALV-J in China have caused substantial losses to the poultry industry.  Specialized detection assays have been developed to combat ALV-J infections in China.  Additionally, ongoing research aims to employ gene-editing technology as a novel antiviral strategy to control the spread of ALV infections.  This review highlights the importance of understanding the impact of ALV-J on the Chinese poultry industry and emphasizes the need for ongoing research and innovation to safeguard poultry health and promote sustainable poultry farming practices in China.

Crop Science

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OsNCED3 and OsPYL1 promote the closure of rice florets by regulating sugar transporters through endogenous abscisic acid Xiawan Zhai, Wenbin Kai, Youming Huang, Jinyin Chen, Xiaochun Zeng 2025, 24(2): 441-452.  DOI: 10.1016/j.jia.2023.11.004 Abstract ( )   PDF in ScienceDirect   Rice is the world’s largest food crop, but it often encounters flowering asynchronization problems during hybrid rice seed production.  In addition, the slow closure of female florets leads to seed mildew and affects the quality.  The hormone abscisic acid (ABA) plays a crucial role in plant responses to abiotic stresses.  Previous studies showed that exogenous ABA promotes floret closure, although the molecular mechanisms and effects of endogenous ABA on floret closure remain unknown.  In this study, the effect of endogenous ABA on floret closure and the molecular mechanism by which ABA promotes floret closure through sugar transporters were investigated by changing the expression levels of OsNCED3 and OsPYL1 in rice.  The results showed that overexpression (OE)-OsNCED3 increased the endogenous ABA level of florets.  Florets closed 5.91 min earlier and OsNCED3 gene knockout line delayed the closure of florets by 5.08 min compared with the wild type.  In addition, OsPYL1 regulated the endogenous ABA content and changed the sensitivity to ABA such that the floret closure times for OE and CRISPR-Cas9 (CR) were 9.84 min earlier and 12.78 min later, respectively, resulting in an increase in the split husk rate to 15.4%.  The gene expression levels of some sugar transporters (STs) changed.  The OsPYL1 and OsSWEET4 proteins could interact on the cell membrane.  These results indicate that ABA promotes the closure of rice florets and the enhanced sensitivity to ABA promotes this effect even more.  The molecular mechanism is mainly related to downstream sugar transporters that respond to the ABA signaling pathway, especially OsSWEET4.

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A genome-wide association study and transcriptome analysis reveal the genetic basis for the Southern corn rust resistance in maize Yang Wang, Chunhua Mu, Xiangdong Li, Canxing Duan, Jianjun Wang, Xin Lu, Wangshu Li, Zhennan Xu, Shufeng Sun, Ao Zhang, Zhiqiang Zhou, Shenghui Wen, Zhuanfang Hao, Jienan Han, Jianzhou Qu, Wanli Du, Fenghai Li, Jianfeng Weng 2025, 24(2): 453-466.  DOI: 10.1016/j.jia.2023.10.039 Abstract ( )   PDF in ScienceDirect   Southern corn rust (SCR) is an airborne fungal disease caused by Puccinia polysora Underw. (P. polysora) that adversely impacts maize quality and yields worldwide.  Screening for new elite SCR-resistant maize loci or genes has the potential to enhance overall resistance to this pathogen.  Using phenotypic SCR resistance-related data collected over two years and three geographical environments, a genome-wide association study was carried out in this work, which eventually identified 91 loci that were substantially correlated with SCR susceptibility.  These included 13 loci that were significant in at least three environments and overlapped with 74 candidate genes (B73_RefGen_v4).  Comparative transcriptomic analyses were then performed to identify the genes related to SCR infection, with 2,586 and 797 differentially expressed genes (DEGs) ultimately being identified in the resistant Qi319 and susceptible 8112 inbred lines following P. polysora infection, respectively, including 306 genes common to both lines.  Subsequent integrative multi-omics investigations identified four potential candidate SCR response-related genes.  One of these genes is ZmHCT9, which encodes the protein hydroxycinnamoyl transferase 9.  This gene was up-regulated in susceptible inbred lines and linked to greater P. polysora resistance as confirmed through cucumber mosaic virus (CMV)-based virus induced-gene silencing (VIGS) system-mediated gene silencing.  These data provide important insights into the genetic basis of the maize SCR response.  They will be useful for for future research on potential genes related to SCR resistance in maize.

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Mapping QTLs for fiber- and seed-related traits in Gossypium tomentosum CSSLs with a G. hirsutum background  Yongshui Hao, Xueying Liu, Qianqian Wang, Shuxin Wang, Qingqing Li, Yaqing Wang, Zhongni Guo, Tiantian Wu, Qing Yang, Yuting Bai, Yuru Cui, Peng Yang, Wenwen Wang, Zhonghua Teng, Dexin Liu, Kai Guo, Dajun Liu, Jian Zhang, Zhengsheng Zhang 2025, 24(2): 467-479.  DOI: 10.1016/j.jia.2024.02.023 Abstract ( )   PDF in ScienceDirect   Introducing the inherent genetic diversity of wild species into cultivars has become one of the hot topics in crop genetic breeding and genetic resource research.  Fiber- and seed-related traits, which are critical to the global economy and people’s livelihoods, are the principal focus of cotton breeding.  Here, the wild cotton species Gossypium tomentosum was used to broaden the genetic basis of G. hirsutum and identify QTLs for fiber- and seed-related traits.  A population of 559 chromosome segment substitution lines (CSSLs) was established with various chromosome segments from G. tomentosum in a G. hirsutum cultivar background.  Totals of 72, 89, and 76 QTLs were identified for three yield traits, five fiber quality traits, and six cottonseed nutrient quality traits, respectively.  Favorable alleles of 104 QTLs were contributed by G. tomentosum.  Sixty-four QTLs were identified in two or more environments, and candidate genes for three of them were further identified.  The results of this study contribute to further studies on the genetic basis of the morphogenesis of these economic traits, and indicate the great breeding potential of G. tomentosum for improving the fiber- and seed-related traits in G. hirsutum.

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Responses of yield, root traits and their plasticity to the nitrogen environment in nitrogen-efficient cultivars of drip-irrigated rice Qingyun Tang, Guodong Wang, Lei Zhao, Zhiwen Song, Yuxiang Li 2025, 24(2): 480-496.  DOI: 10.1016/j.jia.2023.12.014 Abstract ( )   PDF in ScienceDirect   The responses of drip-irrigated rice physiological traits to water and fertilizers have been widely studied.  However, the responses of yield, root traits and their plasticity to the nitrogen environment in different nitrogen-efficient cultivars are not fully understood.  An experiment was conducted from 2020–2022 with a high nitrogen use efficiency (high-NUE) cultivar (T-43) and a low-NUE cultivar (LX-3), and four nitrogen levels (0, 150, 300, and 450 kg ha–1) under drip irrigation in large fields.  The aim was to study the relationships between root morphology, conformation, biomass, and endogenous hormone contents, yield and NUE.  The results showed three main points: 1) Under the same N application rate, compared with LX-3, the yield, N partial factor productivity (PFP), fine root length density (FRLD), shoot dry weight (SDW), root indole-3-acetic acid (IAA), and root zeatin and zeatin riboside (Z+ZR) of T-43 were significantly greater by 11.4–18.9, 11.3–13.5, 11.6–15.7, 9.9–31.1, 6.1–48.1, and 22.8–73.6%, respectively, while the root–shoot ratio (RSR) and root abscisic acid (ABA) were significantly lower (P<0.05); 2) nitrogen treatment significantly increased the rice root morphological indexes and endogenous hormone contents (P<0.05).  Compared to N0, the yield, RLD, surface area density (SAD), root volume density (RVD), and root endogenous hormones (IAA, Z+ZR) were significantly increased in both cultivars under N2 by 61.6–71.6, 64.2–74.0, 69.9–105.6, 6.67–9.91, 54.0–67.8, and 51.4–58.9%, respectively.  Compared with N3, the PFP and N agronomic efficiency (NAE) of nitrogen fertilizer under N2 increased by 52.3–62.4 and 39.2–63.0%, respectively; 3) the responses of root trait plasticity to the N environment significantly differed between the cultivars (P<0.05).  Compared with LX-3, T-43 showed a longer root length and larger specific surface area, which is a strategy for adapting to changes in the nutrient environment.  For the rice cultivar with high-NUE, the RSR was optimized by increasing the FRLD, root distribution in upper soil layers, and root endogenous hormones (IAA, Z+ZR) under suitable nitrogen conditions (N2).  An efficient nutrient acquisition strategy can occur through root plasticity, leading to greater yield and NUE.

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Brassinosteroids alleviate wheat floret degeneration under low nitrogen stress by promoting the redistribution of sucrose from stems to spikes Zimeng Liang, Xidan Cao, Rong Gao, Nian Guo, Yangyang Tang, Vinay Nangia, Yang Liu 2025, 24(2): 497-516.  DOI: 10.1016/j.jia.2023.12.017 Abstract ( )   PDF in ScienceDirect   The trade-off between yield and environmental effects caused by nitrogen fertilizer application is an important issue in wheat production.  A reduction in fertile florets is one of the main reasons for the lower yields under low nitrogen application rates.  Brassinosteroids (BRs) have been found to play a role in nitrogen-induced rice spikelet degeneration.  However, whether BRs play a role in wheat floret development and the mechanisms involved are not clear.  Therefore, a nitrogen gradient experiment and exogenous spraying experiment were conducted to investigate the role and mechanism of BRs in wheat floret development under low nitrogen stress.  The results showed that as the nitrogen application decreased, the endogenous BRs content of the spikes decreased, photosynthesis weakened, and total carbon, soluble sugar and starch in the spikes decreased, leading to a reduction in the number of fertile florets.  Under low nitrogen stress, exogenous spraying of 24-epibrassinolide promoted photosynthesis, and stimulated stem fructan hydrolysis and the utilization and storage of sucrose in spikes, which directed more carbohydrates to the spikes and increased the number of fertile florets.  In conclusion, BRs mediate the effects of nitrogen fertilizer on wheat floret development, and under low nitrogen stress, foliar spraying of 24-epibrassinolide promotes the flow of carbohydrates from the stem to the spikes, alleviating wheat floret degeneration.

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Enhancing carbon sequestration and greenhouse gas mitigation in semiarid farmland: The promising role of biochar application with biodegradable film mulching Jinwen Pang, Zhonghong Tian, Mengjie Zhang, Yuhao Wang, Tianxiang Qi, Qilin Zhang, Enke Liu, Weijun Zhang, Xiaolong Ren, Zhikuan Jia, Kadambot H. M. Siddique, Peng Zhang 2025, 24(2): 517-526.  DOI: 10.1016/j.jia.2023.12.011 Abstract ( )   PDF in ScienceDirect   Long-term mulching has improved crop yields and farmland productivity in semiarid areas, but it has also increased greenhouse gas (GHG) emissions and depleted soil fertility.  Biochar application has emerged as a promising solution for addressing these issues.  In this study, we investigated the effects of four biochar application rates (no biochar (N)=0 t ha–1, low (L)=3 t ha–1, medium (M)=6 t ha–1, and high (H)=9 t ha–1) under film mulching and no mulching conditions over three growing seasons.  We assessed the impacts on GHG emissions, soil organic carbon sequestration (SOCS), and maize yield to evaluate the productivity and sustainability of farmland ecosystems.  Our results demonstrated that mulching increased maize yield (18.68–41.80%), total fixed C in straw (23.64%), grain (28.87%), and root (46.31%) biomass, and GHG emissions (CO2, 10.78%; N2O, 3.41%), while reducing SOCS (6.57%) and GHG intensity (GHGI; 13.61%).  Under mulching, biochar application significantly increased maize yield (10.20%), total fixed C in straw (17.97%), grain (17.69%) and root (16.75%) biomass, and SOCS (4.78%).  Moreover, it reduced the GHG emissions (CO2, 3.09%; N2O, 6.36%) and GHGI (12.28%).  These effects correlated with the biochar addition rate, with the optimal rate being 9.0 t ha–1.  In conclusion, biochar application reduces CO2 and N2O emissions, enhances CH4 absorption, and improves maize yield under film mulching.  It also improves the soil carbon fixation capacity while mitigating the warming potential, making it a promising sustainable management method for mulched farmland in semiarid areas.

Horticulture

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The CONSTANS-LIKE SlCOL1 in tomato regulates the fruit chlorophyll content by stabilizing the GOLDEN2-LIKE protein Long Cui, Fangyan Zheng, Chenhui Zhang, Sunan Gao, Jie Ye, Yuyang Zhang, Taotao Wang, Zonglie Hong, Zhibiao Ye, Junhong Zhang 2025, 24(2): 536-545.  DOI: 10.1016/j.jia.2024.11.022 Abstract ( )   PDF in ScienceDirect   CONSTANS (CO) and CONSTANS-LIKE (COL) transcription factors are known to regulate a series of cellular processes, including the transition from vegetative growth to flower development in plants.  However, their role in regulating the fruit chlorophyll content is poorly understood.  In this study, SlCOL1, the tomato (Solanum lycopersicum) ortholog of Arabidopsis CONSTANS, was shown to play key roles in controlling fruit chlorophyll.  The suppression of SlCOL1 expression led to a reduction in the chlorophyll content of immature green fruit, while the overexpression of SlCOL1 increased it.  An analysis of protein–protein interactions indicated that SlCOL1 forms a complex with GOLDEN2-LIKE (GLK2), which promotes the stability of its protein.  The overexpression of SlCOL1 in the glk2 null mutation background of tomato failed to promote chlorophyll accumulation in the immature green fruit, which suggests that GLK2 is required for the function of SlCOL1 in regulating chlorophyll content.  These results shed new light on the mechanisms used by COL1 and GLK2 to regulate fruit development and chlorophyll accumulation in tomato.

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Abscisic acid alleviates photosynthetic damage in the tomato ABA-deficient mutant sitiens and protects photosystem II from damage via the WRKY22–PsbA complex under low-temperature stress Jiamao Gu, Pengkun Liu, Wenting Nie, Zhijun Wang, Xiaoyu Cui, Hongdan Fu, Feng Wang, Mingfang Qi, Zhouping Sun, Tianlai Li, Yufeng Liu 2025, 24(2): 546-563.  DOI: 10.1016/j.jia.2024.11.040 Abstract ( )   PDF in ScienceDirect   Abscisic acid (ABA) plays a key role in promoting the growth and development of plants, as well as mediating the responses of plants to adverse environmental conditions.  Here, we measured the photosynthetic capacity of wild-type RR, mutant sitiens (sit), and ABA-pretreated sit tomato seedlings following exposure to low-temperature (LT) stress.  We found that the net photosynthetic rate, intercellular carbon dioxide concentration, transpiration rate, and stomatal conductance of sit seedlings were lower than those of RR seedlings under LT stress.  The chloroplast width, area, and number of osmiophilic granules were significantly larger in sit seedlings than in RR seedlings, while the chloroplast length/width ratio was significantly lower in sit seedlings than in RR seedlings.  The photochemical activity of sit seedlings was lower, and the expression of photosynthesis-related genes in sit seedlings was altered following exposure to LT stress.  ABA pretreatment significantly alleviated the above phenomenon.  We also conducted an RNA sequencing analysis and characterized the expression patterns of genes in tomato seedlings following exposure to LT stress.  We constructed 15 cDNA libraries and identified several differentially expressed genes involved in photosynthesis, plant hormone signaling transduction, and primary and secondary metabolism.  Additional analyses of genes encoding transcription factors and proteins involved in photosynthesis-related processes showed pronounced changes in expression under LT stress.  Luciferase reporter assay and electrophoretic mobility shift assay revealed that WRKY22 regulates the expression of PsbA.  The PSII of WRKY22 and PsbA-silenced plants was inhibited.  Our findings indicate that ABA plays a role in regulating the process of photosynthesis and protecting PSII in tomato under LT stress through the WRKY22–PsbA complex.

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Functional analysis of tomato MAP65 gene family, highlighting SlMAP65-1’s role in fruit morphogenesis Peiyu Zhang, Guoning Zhu, Chunjiao Zhang, Hongliang Zhu 2025, 24(2): 564-574.  DOI: 10.1016/j.jia.2024.07.014 Abstract ( )   PDF in ScienceDirect   The plant growth process is accompanied by dynamic changes in the microtubules, and the rearrangement of microtubules is regulated by diverse microtubule-associated proteins (MAPs).  Plant MAP65s have been exhaustively characterized in some species, but the information about MAP65 family members in tomato (Solanum lycopersicum) is limited.  In this study, nine SlMAP65 family genes were identified in the tomato genome.  Then a systematic analysis that considered the physio-chemical properties, evolution, conserved motifs, domains, gene structure, and cis-regulatory elements of SlMAP65 family members was conducted.  The family member SlMAP65-1, which had the highest expression, was knocked out by CRISPR/Cas9.  The tomato fruit of slmap65-1 loss of function lines showed an elongated morphology, and the data indicated that SlMAP65-1 is involved in fruit morphogenesis at the early fruit development stage.  These results provide new insights for fruit morphogenesis-related research and future functional studies of the SlMAP65 family members in tomato.

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HpFBH3 transactivates HpCO7 via binding to the E-boxes in the promoter and may accelerate flower formation in pitaya Xiaowei Cai, Ling Xiao, Xiangmei Nie, Qiandong Hou, Sulin Wen, Kun Yang, Xiaopeng Wen 2025, 24(2): 575-593.  DOI: 10.1016/j.jia.2024.07.039 Abstract ( )   PDF in ScienceDirect   Hylocereus polyrhizus, also known as pitaya or dragon fruit, is a climbing cactus grown worldwide because of its excellent performance under drought stress and appealing red-purple fruits.  In practice, accelerating flower formation and inducing more flowers usually result in higher yield.  However, the genes for this purpose have not been well characterized in pitaya.  Previously, FLOWERING BHLHs (FBHs) have been identified as positive regulators of flower formation.  In the present work, a total of eight FBHs were identified in pitaya.  This is a greater number than in beet and spinach, possibly because of the recent whole-genome duplication that occurred in the pitaya genome.  The phylogenetic tree indicated that the FBHs could be divided into three groups.  In TYPE II, the genes of Caryophyllales encode atypical FBHs and are generated by dispersed duplication.  The Ka/Ks ratios indicated that HpFBHs are under purifying selection.  Promoter and expression analysis of HpFBHs revealed that they are spatiotemporally activated in flower-related tissues and responsive to multiple abiotic stresses.  These results indicated that HpFBHs are involved in the flower formation of pitaya.  Therefore, typical HpFBH1/3 from TYPE III and an atypical HpFBH8 from TYPE II were selected for functional verification.  HpFBH3 was found to heterodimerize with HpFBH1 in the nucleus using subcellular localization, yeast two-hybrid and luciferase complementation assays.  With bioinformatic analysis, all HpFBHs were predicted to transactivate downstream genes via binding to the E-boxes, which were frequently detected in the promoters of HpCOs, HpFTs and HpSOC1s.  RNA-Seq datasets showed that these flowering accelerators were expressed in coordination with HpFBH3.  Yeast one-hybrid and dual-luciferase reporter assays further verified that HpFBH3 transactivated HpCO7 by selectively binding to the E-boxes in the promoter.  Moreover, ectopic overexpression of HpFBH3 accelerated flower formation in Arabidopsis.  In summary, this study systematically characterized the typical HpFBHs, especially HpFBH3, as positive regulators of flower formation, which could be target genes for the genetic improvement of pitaya.

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Development of an Agrobacterium tumefaciens-mediated transformation system for somatic embryos and transcriptome analysis of LcMYB1’s inhibitory effect on somatic embryogenesis in Litchi chinensis Yaqi Qin, Bo Zhang, Xueliang Luo, Shiqian Wang, Jiaxin Fu, Zhike Zhang, Yonghua Qin, Jietang Zhao, Guibing Hu 2025, 24(2): 594-609.  DOI: 10.1016/j.jia.2024.03.007 Abstract ( )   PDF in ScienceDirect   Litchi has great economic significance as a global fruit crop.  However, the advancement of litchi functional genomics has encountered substantial obstacles due to its recalcitrance to stable transformation.  Here, we present an efficacious Agrobacterium tumefaciens-mediated transformation system in somatic embryos of ‘Heiye’ litchi.  This system was developed through the optimization of key variables encompassing explant selection, A. tumefaciens strain delineation, bacterium concentration, infection duration, and infection methodology.  The subsequent validation of the transformation technique in litchi was realized through the ectopic expression of LcMYB1, resulting in the generation of transgenic calli.  However, the differentiation of transgenic calli into somatic embryos encountered substantial challenges.  To delineate the intricate molecular underpinnings of LcMYB1’s inhibitory role in somatic embryo induction, a comprehensive transcriptome analysis was conducted that encompassed embryogenic calli (C), globular embryos (G), and transgenic calli (TC).  A total of 1,166 common differentially expressed genes (DEGs) were identified between C-vs.-G and C-vs.-TC.  Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that these common DEGs were mostly related to plant hormone signal transduction pathways.  Furthermore, RT-qPCR corroborated the pronounced down-regulation of numerous genes that are associated with somatic embryo induction within the transgenic calli.  The development of this transformation system provides valuable support for functional genomics research in litchi.

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PrDA1-1 and its interacting proteins PrTCP1/PrTCP9 in flare tree peony affect yield by regulating seed weight and number Chunyan He, Fangyun Cheng 2025, 24(2): 610-622.  DOI: 10.1016/j.jia.2024.10.001 Abstract ( )   PDF in ScienceDirect   Tree peony is an emerging woody oilseed crop that has the potential to help meet the growing demand for edible oil and biofuel, and breeding high-yield varieties is the key to increasing production.  The DA1 gene regulates seed weight and number and effectively improves crop yield, although its function in tree peonies has not yet been reported.  In this study, three PrDA1 genes were first identified in the whole genome of ‘Jing Hong’ flare tree peony (Paeonia rockii).  PrDA1-1 is homologous to AtDA1 and contains three types of conserved structural domains: UIM (ubiquitin-interacting motif), LIM (Lin11, Isl-1, and Mec-3), and DA-like.  PrDA1-1 was strongly expressed during both the early and late stages of seed development, and its constitutive expression in Arabidopsis reduced seed number but increased seed weight, which ultimately led to a reduction in yield.  PrDA1-1 was found to interact with PrTCP1/PrTCP9 in yeast and plants, and the constitutive expression of PrTCP1/PrTCP9 in Arabidopsis increased seed weight and number, which resulted in higher yield.  Since this study showed that PrDA1-1 and PrTCP1/PrTCP9 regulate seed weight and number to affect yield, these new genetic resources will help to improve the seed yield of tree peonies.

Plant Protection

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Point mutations of Dicer2 conferred Fusarium asiaticum resistance to RNAi-related biopesticide Kaixin Gu, Ran Wei, Yidan Sun, Xiaoxin Duan, Jing Gao, Jianxin Wang, Yiping Hou, Mingguo Zhou, Xiushi Song 2025, 24(2): 623-637.  DOI: 10.1016/j.jia.2023.10.024 Abstract ( )   PDF in ScienceDirect   The use of RNA interference (RNAi) technology to control pests is explored by researchers globally.  Even though RNA is a new class of pest control compound unlike conventional chemical pesticides, the evolution of pest resistance needs to be considered.  Here, we first investigate RNAi-based biopesticide resistance of Fusarium asiaticum, which is responsible for devastating diseases of plants, for example, Fusarium head blight.  Five resistant strains were isolated from 500 strains that treated with UV-mutagenesis.  The mutation common to all of the five resistant mutants occurred in the gene encoding Dicer2 (point mutations at codon 1005 and 1007), which were under strong purifying selection pressure.  To confirm whether the mutations in Dicer2 confer resistance to RNAi, we exchanged the Dicer2 locus between the sensitive strain and the resistant strain by homologous double exchange.  The transformed mutants, Dicer2R1005D and Dicer2E1007H, exhibited resistance to dsRNA in vitro.  Further study showed that mutations of R1005D and E1007H affected the intramolecular interactions of Dicer2, resulting in the dysfunction of RNase III domain of Dicer2.  The amount of sRNAs produced by Dicer2R1005D and Dicer2E1007H was extremely reduced along with variation of sRNA length.  Together, these findings revealed a new potential mechanism of RNAi resistance and provided insight into RNAi-related biopesticide deployment for fungal control.

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Uncoupling of nutrient metabolism and cellular redox by cytosolic routing of the mitochondrial G-3-P dehydrogenase Gpd2 causes loss of conidiation and pathogenicity in Pyricularia oryzae Wenqin Fang, Yonghe Hong, Tengsheng Zhou, Yangdou Wei, Lili Lin, Zonghua Wang, Xiaohan Zhu 2025, 24(2): 638-654.  DOI: 10.1016/j.jia.2024.05.021 Abstract ( )   PDF in ScienceDirect   Oxidation of self-stored carbohydrates and lipids provides the energy for the rapid morphogenetic transformation during asexual and infection-related development in Pyricularia oryzae, which results in intracellular accumulation of reducing equivalents NADH and FADH2, requiring a cytosolic shuttling machinery towards mitochondria.  Our previous studies identified the mitochondrial D-lactate dehydrogenase MoDld1 as a regulator to channel the metabolite flow in conjunction with redox homeostasis.  However, the regulator(s) facilitating the cytosolic redox balance and the importance in propelling nutrient metabolite flow remain unknown.  The G-3-P shuttle is a conserved machinery transporting the cytosolic reducing power to mitochondria.  In P. oryzae, the mitochondrial G-3-P dehydrogenase Gpd2 was required for cellular NAD+/NADH balance and fungal virulence.  In this study, we re-locate the mitochondrial G-3-P dehydrogenase Gpd2 to the cytosol for disturbing cytosolic redox status.  Our results showed overexpression of cytosolic gpd2Δmts without the mitochondrial targeted signal (MTS) driven by Ribosomal protein 27 promoter (PR27) exerted conflicting regulation of cellular oxidoreductase activities compared to the ΔModld1 deletion mutant by RNA-seq and prevented the conidiation and pathogenicity of P. oryzae.  Moreover, overexpression of gpd2Δmts caused defects in glycogen and lipid mobilization underlying asexual and infectious structural development associated with decreased cellular NADH production and weakened anti-oxidation activities.  RNA-seq and non-targeted metabolic profiling revealed down-regulated transcriptional activities of carbohydrate metabolism and lower abundance of fatty acids and secondary metabolites in RP27:gpd2Δmts.  Thus, our studies indicate the essential role of cytosolic redox control in nutrient metabolism fueling the asexual and infection-related development in P. oryzae.

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Characterization of core maize volatiles induced by Spodoptera frugiperda that alter the mating-mediated approach–avoidance behaviors of Mythimna separata Denghai Yang, Hengzhe Fan, Ruyi Hu, Yong Huang, Chengwang Sheng, Haiqun Cao, Bin Yang, Xingchuan Jiang 2025, 24(2): 655-667.  DOI: 10.1016/j.jia.2024.05.029 Abstract ( )   PDF in ScienceDirect   The fall armyworm (Spodoptera frugiperda) is an invasive species and a destructive pest of maize, which significantly impacts native species and communities via complex mechanisms like competition for resources. However, the interaction between S. frugiperda and local pests remains unclear. In this study, we determined that Oriental armyworm (Mythimna separata) females with different mating status displayed different approach-avoidance behaviors towards maize which was damaged by S. frugiperda larvae. The virgin M. separata females were repelled, while the mated females were attracted by the S. frugiperda-damaged maize. To further understand the olfactory mechanism of this phenomenon, seven volatiles induced by S. frugiperda in maize were characterized by gas chromatography and mass spectrometry (GC-MS), including trans-2-hexenal, linalool, trans-β-farnesene, cis-3-hexenyl acetate, β-caryophyllene, trans-α-bergamotene, and isopentyl acetate. Additionally, electrophysiological and behavioral assays of the seven compounds were performed using both virgin and mated females of M. separata. We determined that virgin and mated females displayed different responses to the HIPV compounds. Trans-β-farnesene was the core compound for repelling virgin females, and trans-2-hexenal was the key attractant for oviposition in mated female M. separata individuals. These findings help our comprehension of the relationships between maize pests and offer new possibilities for controlling them by olfactory-based strategies.

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Transcriptome-based analysis reveals chromatin remodeling in post-adult eclosion reconstruction of the insect fat body Yiying Li, Yuanyuan Hu, Bei Wang, Mengyao Lang, Shutang Zhou, Zhongxia Wu 2025, 24(2): 668-679.  DOI: 10.1016/j.jia.2024.06.018 Abstract ( )   PDF in ScienceDirect   The insect fat body is comparable to the liver and adipose tissue in vertebrates, and plays a pivotal role in energy metabolism, nutrient storage, and reproduction.  During metamorphosis, the fat body is disassembled via programmed cell death and cell dissociation.  After adult eclosion, the fat body is reconstructed either by repopulation from the remaining juvenile fat body cells or by differentiation from adult progenitor cells.  This reconstruction is a prerequisite for initiating the extensive synthesis of vitellogenin (Vg), which is necessary for the maturation of eggs.  Despite its significance, the underlying mechanisms of this reconstruction remain inadequately understood.  Transcriptome analysis of the fat bodies from migratory locusts at 0–5 days post adult emergence revealed 79 genes associated with chromatin remodeling.  Weighted gene co-expression network analysis indicated a positive correlation between chromatin remodeling and fat body reconstitution.  Protein–protein interaction analysis revealed that brahma, which encodes the catalytic subunit of the SWI/SNF chromatin remodeling complex, is crucial for post-adult-eclosion fat body development.  qRT-PCR analysis demonstrated that the levels of brahma mRNA in the fat body are progressively increased during the previtellogenic stage, then reach the peak and remain elevated in the vitellogenic phase.  Furthermore, brahma is expressed in response to gonadotropic juvenile hormone (JH).  Knockdown of brahma led to a marked reduction in Vg expression within the fat body, along with arrested ovarian growth.  These findings shed light on the involvement of brahma-mediated chromatin remodeling in JH-stimulated fat body reconstruction and reproduction of adult female locusts.

Animal Science · Veterinary Medicine

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Expression of tissue factor pathway inhibitor 2 in the follicles of chicken ovaries and its regulatory mechanism in cultured granulosa cells Chong Li, Yangguang Ren, Yudian Zhao, Zihao Zhang, Bin Zhai, Jing Li, Qi Li, Guoxi Li, Zhuanjian Li, Xiaojun Liu, Xiangtao Kang, Ruirui Jiang, Yadong Tian, Donghua Li 2025, 24(2): 680-696.  DOI: 10.1016/j.jia.2023.06.038 Abstract ( )   PDF in ScienceDirect   Tissue factor pathway inhibitor 2 (TFPI2) plays a key role in female reproduction.  However, its expression and function in chickens are still unclear.  In this study, RNA-seq was performed on ovarian tissues from chickens aged 30 and 15 weeks to identify the differentially expressed gene TFPI2.  The full-length cDNA of TFPI2 was obtained from adult chicken ovaries by rapid-amplification of cDNA ends (RACE), and the putative TFPI2 protein was found to share a highly conserved amino acid sequence with known bird homologs.  In addition, TFPI2 was widely expressed in the tissues of adult chicken follicles according to quantitative real-time PCR (qRT-PCR) and Western blotting.  Immunohistochemistry suggested that the TFPI2 protein existed in chicken ovary follicles at different developmental states, such as primordial follicles, the ovarian stroma, and the granulosa and theca layers of prehierarchical follicles (6–8 mm) and preovulatory follicles (F1).  In vitro, follicle stimulating hormone or luteinizing hormone (FSH/LH) stimulated the expression of TFPI2 in chicken granulosa cells.  FSH-/LH-induced TFPI2 mRNA expression was mediated by signaling pathways such as the PKA, PKC, PI3K, and mTOR pathways.  Functionally, TFPI2 promoted the proliferation and viability of cultured granulosa cells and decreased the secretion of Progesterone (P4) and Estrogen (E2) and the mRNA abundance of key steroidogenic enzymes (STAR, Cyp17a1, Cyp19a1 and 3B-HSD) as well as MMPs (MMP2, 7, 9 and 11).  Mechanistically, TFPI2 inhibited the expression of MMP7 via the Wnt signaling pathway.  These findings indicate that TFPI2 may play an important role in regulating chicken follicular development and ovulation and suggest the molecular regulation mechanisms.

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Genome-wide circular RNAs signatures involved in sexual maturation and its heterosis in chicken Yuanmei Wang, Jingwei Yuan, Yanyan Sun, Aixin Ni, Jinmeng Zhao, Yunlei Li, Panlin Wang, Lei Shi, Yunhe Zong, Pingzhuang Ge, Shixiong Bian, Hui Ma, Jilan Chen 2025, 24(2): 697-711.  DOI: 10.1016/j.jia.2023.05.026 Abstract ( )   PDF in ScienceDirect   Sexual maturation heterosis has been widely exploited in animal crossbreeding.  However, the underlying mechanism has been rarely explored in chicken.  In the present study, we performed the reciprocal crossing between White Leghorn and Beijing You chicken to evaluate the phenotypes related to sexual maturation, and profiled the ovary circRNAs of purebreds (WW, YY) and crossbreds (WY, YW) to elucidate the molecular mechanism underlying heterosis for sexual maturation.  Pubic space and oviduct length exhibited positive heterosis, and age at first egg (AFE) exhibited negative heterosis in the crossbreds.  We identified 3,025 known circRNAs and 624 putative circRNAs, which were mainly derived from the exons.  Among these circRNAs, 141 and 178 circRNAs were specially expressed in WY and YW, respectively.  There were 52.38 and 64.63% of total circRNAs in WY and YW exhibited non-additive expression pattern, respectively.  GO enrichment and KEGG pathway analysis showed that the host genes of non-additive circRNAs were mainly involved in TGF-beta signaling pathway, oocyte development, ATPase activator activity, oocyte meiosis, progesterone-mediated oocyte maturation and GnRH signaling pathway.  Weighted gene co-expression network analysis identified that 4 modules were significantly (P<0.05) correlated with oviduct length and pubic space.  The host genes of non-additive circRNAs harbored in the 4 modules were associated with MAPK signaling pathway and Wnt signaling pathway.  Furthermore, competing endogenous RNAs (ceRNA) network analysis characterized non-additive circRNAs gal-FGFR2_0005 and gal-MAPKAP1_0004 could interact with gga-miR-1612 and gga-miR-12235-5p to regulate CNOT6, COL8A1, and FHL2, which were essential for ovary development, indicating that the non-additive circRNAs involved in the formation of sexual maturation heterosis through regulating genes related to the reproductive and developmental process.  The findings would provide a deeper understanding of the molecular mechanism underlying sexual maturation heterosis from a novel perspective.

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Velogenic Newcastle disease virus invades chicken brain by infecting brain microvascular endothelial cells to increase blood-brain barrier permeability Jie Zhao, Sa Xiao, Wei Yao, Xudong Chang, Xinglong Wang, Zengqi Yang, Wenbin Wang 2025, 24(2): 712-723.  DOI: 10.1016/j.jia.2024.06.003 Abstract ( )   PDF in ScienceDirect   The blood-brain barrier (BBB) keeps poisons and infections out of the brain.  Some viruses can pass through this barrier and replicate in the central nervous system (CNS).  Velogenic Newcastle disease virus (VNDV) is a neurotropic virus that causes avian nonsuppurative encephalitis.  VNDV often develops into a chronic infection that seriously affects poultry health in partially immune birds.  The routes by which the virus enters the chicken brain are poorly understood.  In this study, we discovered that VNDV increased BBB permeability in vivo and in vitro by breaking the tight junction protein zona occludens-1 (ZO-1) continuity of chicken brain microvascular endothelial cells (chBMECs).  By investigating the susceptibility of chBMECs to NDV infection, we found that VNDV but not lentogenic NDV was detected in the basolateral compartment in transwell assays after apical infection, suggesting that efficient replication and transcellular transport of the virus across the BBB in vitro.  Furthermore, viral replication and BBB permeability were reduced during the early stage of infection by using the dynamin inhibitor dynasore.  Our data demonstrate that VNDV invades the chicken brain by infecting and damaging the tight junction of chBMECs directly to increase BBB permeability.  VNDV could infect chBMECs via endocytosis.  As a result, our findings provide compelling evidence for VNDV entrance into the brain via the BBB, paving the way for the development of medications for NDV prevention and therapy.

Agro-ecosystem & Environment

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Water deficit affects the nitrogen nutrition index of winter wheat under controlled water conditions Ben Zhao, Anzhen Qin, Wei Feng, Xinqiang Qiu, Pingyan Wang, Haixia Qin, Yang Gao, Guojie Wang, Zhandong Liu, Syed Tahir Ata-Ul-Karim 2025, 24(2): 724-738.  DOI: 10.1016/j.jia.2024.08.027 Abstract ( )   PDF in ScienceDirect   Nitrogen (N) uptake is regulated by water availability, and a water deficit can limit crop N responses by reducing N uptake and utilization.  The complex and multifaceted interplay between water availability and the crop N response makes it difficult to predict and quantify the effect of water deficit on crop N status.  The nitrogen nutrition index (NNI) has been widely used to accurately diagnose crop N status and to evaluate the effectiveness of N application.  The decline of NNI under water-limiting conditions has been documented, although the underlying mechanism governing this decline is not fully understood.  This study aimed to elucidate the reason for the decline of NNI under water-limiting conditions and to provide insights into the accurate utilization of NNI for assessing crop N status under different water–N interaction treatments.  Rainout shelter experiments were conducted over three growing seasons from 2018 to 2021 under different N (75 and 225 kg N ha–1, low N and high N) and water (120 to 510 mm, W0 to W3) co-limitation treatments.  Plant N accumulation, shoot biomass (SB), plant N concentration (%N), soil nitrate-N content, actual evapotranspiration (ETa), and yield were recorded at the stem elongation, booting, anthesis and grain filling stages.  Compared to W0, W1 to W3 treatments exhibited NNI values that were greater by 10.2 to 20.5%, 12.6 to 24.8%, 14 to 24.8%, and 16.8 to 24.8% at stem elongation, booting, anthesis, and grain filling, respectively, across the 2018–2021 seasons.  This decline in NNI under water-limiting conditions stemmed from two main factors.  First, reduced ETa and SB led to a greater critical N concentration (%Nc) under water-limiting conditions, which contributed to the decline in NNI primarily under high N conditions.  Second, changes in plant %N played a more significant role under low N conditions.  Plant N accumulation exhibited a positive allometric relationship with SB and a negative relationship with soil nitrate-N content under water-limiting conditions, indicating co-regulation by SB and the soil nitrate-N content.  However, this regulation was influenced by water availability.  Plant N accumulation sourced from the soil nitrate-N content reflects soil N availability.  Greater soil water availability facilitated greater absorption of soil nitrate-N into the plants, leading to a positive correlation between plant N accumulation and ETa across the different water–N interaction treatments.  Therefore, considering the impact of soil water availability is crucial when assessing soil N availability under water-limiting conditions.  The findings of this study provide valuable insights into the factors contributing to the decline in NNI among different water–N interaction treatments and can contribute to the more accurate utilization of NNI for assessing winter wheat N status.

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Preceding crop rotation systems shape the selection process of wheat root-associated bacterial communities Shuting Yu, Tianshu Wang, Li Wang, Shuihong Yao, Bin Zhang 2025, 24(2): 739-753.  DOI: 10.1016/j.jia.2024.07.004 Abstract ( )   PDF in ScienceDirect   Wheat–maize (WM) and wheat–soybean (WS) double-cropping rotation systems are predominant in the North China Plain, with implications for national agricultural output and sustainability.  As rotation systems exert legacy effects on soil health and crop productivity, the role of crop rotation in shaping the root-associated microbiome of the succeeding crops has emerged as a pivotal aspect of crop management research.  Here, the effects of the preceding two cycles of WM and WS rotations on the recruitment and filtering of wheat root-associated bacterial communities across wheat developmental stages were investigated.  Our results revealed that bacterial community diversity and composition were primarily influenced by compartment and developmental stage, while the preceding rotation systems had a slight but significant effect on wheat root-associated bacterial communities.  The co-occurrence networks under WM were more complex in the wheat rhizosphere and rhizoplane, with the operational taxonomic units (OTUs) related to cellulolysis showing greater connectivity.  The co-occurrence networks under WS were simple but stable in the rhizosphere and complex in the rhizoplane and endosphere, with the OTUs related to ureolysis and nitrogen fixation showing greater connectivity.  While both stochastic and deterministic processes contributed to the assembly of wheat root-associated bacterial communities, the contributions of deterministic processes under WS were 19.4–38.5% higher than those under the WM rotation across the root-associated compartments, indicating the substantial impact of a soybean legacy effect on wheat root selection of microbes.  Plant growth-promoting rhizobacteria with the potential to fix nitrogen, produce indole-3-acetic acid, and inhibit diseases such as Betaproteobacteriales, Azospirillales and Dyella sp., were identified within the OTUs that were consistently enriched across all the wheat root-associated compartments and developmental stages, which were also important predictors of wheat yield.  This study elucidates the role of crop rotation in modulating the dynamics of crop root-associated bacterial communities, and underscores the potential of targeted microbiome manipulation for optimizing wheat production and enhancing soil health.

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Anaerobic soil disinfestation rather than Bacillus velezensis Y6 inoculant suppresses tomato bacterial wilt by improving soil quality and manipulating bacterial communities Taowen Pan, Yulin Chen, Sicong Li, Lei Wang, Joji Muramoto, Carol Shennan, Jihui Tian, Kunzheng Cai 2025, 24(2): 754-768.  DOI: 10.1016/j.jia.2024.06.019 Abstract ( )   PDF in ScienceDirect   Continuous cropping leads to high incidence of soilborne diseases such as bacterial wilt caused by Ralstonia solanacearum, which poses a risk to agricultural production.  Anaerobic soil disinfestation (ASD) and plant growth-promoting rhizobacteria (PGPR) are considered environmentally friendly methods to control bacterial wilt.  However, the underlying mechanism of the improvement of soil health and the inhibition of bacterial wilt after ASD treatment and PGPR inoculation needs further exploration.  This study evaluated the effect of ASD treatment on soil improvement at pre-planting of tomato, and the effect of ASD treatment combined with the application of Bacillus velezensis Y6 (BV) on soil quality, R. solanacearum abundance, and bacterial communities at 90 days before harvesting of tomato.  The results showed that ASD treatment reduced R. solanacearum abundance in soil by 17.6% at pre-planting and 18.7% at 90 days before harvesting, but BV inoculation did not influence R. solanacearum abundance.  ASD and ASD+BV treatments effectively reduced the occurrence of bacterial wilt, improved soil nutrient status and increased soil microbial activity at 90 days before harvesting.  Principal co-ordinate analysis showed that the soil bacterial community was significantly influenced by ASD treatment both at pre-planting and at 90 days before harvesting.  Further investigation found that ASD contributed to the enrichment of beneficial flora (Bacillus and Streptomyces).  Moreover, pH was an important environmental factor affecting the abundance of R. solanacearum in soil.  Co-occurrence network analysis showed that ASD treatment significantly increased network connection of bacterial communities and the proportion of beneficial microorganisms (Proteobacteria and Firmicutes), leading to complex soil bacterial co-occurrence networks both at pre-planting and at 90 days before harvesting.  Collectively, these results indicate that ASD treatment, but not microbial inoculation can enhance tomato plant resistance to bacterial wilt by improving soil quality and modulating the soil bacterial community.

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Impact of a new pesticide on rhizosphere microbes and plant health: Case study of Y17991 against sharp eyespot in wheat Xiangxia Yang, Tingting Chen, Libo Xiang, Limin Liu, Mi Wei 2025, 24(2): 769-785.  DOI: 10.1016/j.jia.2024.08.022 Abstract ( )   PDF in ScienceDirect   Sharp eyespot (Rhizoctonia cerealis) is a widespread soil-borne fungal disease that poses a severe threat to wheat health, and it is one of the main obstacles to achieving stable and high-quality wheat yields in China.  Our collaborative team has developed a novel, efficient, and low-toxicity fungicide named Y17991 (N-(2-(2,4-bis-(trifluoromethyl)phenoxy)phenyl)-3-(difluoromethyl)-1-methyl-1H-pyrazole-4-carboxamide).  Preliminary laboratory tests confirmed the significant inhibitory effect of this agent on R. cerealis.  Large-area field trials also demonstrated its efficacy, with a disease prevention index of 83.52%, which is 1.97% greater than that of the widely used thifluzamide, and it significantly increased the wheat yield.  Moreover, this study explored the impacts of Y17991 on the structure and function of the microbial community in wheat rhizosphere soil.  Bacterial communities were more strongly affected than fungal communities.  Y17991 significantly modulated key amino acid metabolic pathways and certain biosynthetic processes in diseased wheat rhizospheres, and it also enhanced certain biosynthetic pathways and metabolic activities in healthy wheat rhizospheres.  Additionally, the application of Y17991 regulated rhizosphere metabolites, thus exerting significant control over the microbial community.  We identified 15 microbial strains potentially involved in the prevention and treatment of R. cerealis, and Y17991 treatment promoted the growth of Pedobacter and Bacillus strains.  These strains not only aid in plant growth but they also have the potential for disease prevention.  In summary, Y17991 application at a reasonable dose does not cause significant disruption to nontarget rhizosphere microbial communities.  In future studies, we will continue to investigate the impacts of Y17991 on nonmicrobial components in soil ecosystems, such as protozoa and nematodes.  Our research provides a theoretical basis for the scientific application and promotion of new fungicides and offers a significant reference for establishing a comprehensive system for assessing the ecological impact of pesticides on the environment.

Letter

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SiEPFs enhance water use efficiency and drought tolerance by regulating stomatal density in foxtail millet (Setaria italica) Jianhong Hao, Xueting Kang, Lingqian Zhang, Jiajing Zhang, Huashuang Wu, Zidong Li, Dan Wang, Min Su, Shuqi Dong, Xiaorui Li, Lulu Gao, Guanghui Yang, Xiaoqian Chu, Xiangyang Yuan, Jiagang Wang 2025, 24(2): 786-789.  DOI: 10.1016/j.jia.2024.09.008 Abstract ( )   PDF in ScienceDirect

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On-site visual detection of Nipah virus combining a reverse transcription recombinase-aided amplification with a lateral-flow dipstick assay Kaikai Jin, Junjie Zhao, Huanxin Chen, Zimo Zhang, Zengguo Cao, Zanheng Huang, Hao Li, Yongsai Liu, Lisi Ai, Yufei Liu, Changqi Fan, Yuanyuan Li, Pei Huang, Hualei Wang, Haili Zhang 2025, 24(2): 790-794.  DOI: 10.1016/j.jia.2024.11.018 Abstract ( )   PDF in ScienceDirect