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| Comprehensive analysis of YABBY gene family in foxtail millet (Setaria italica) and functional characterization of SiDL |
| GUO Jie1, ZHOU Xu-tao1, DAI Ke-li1, YUAN Xiang-yang1, GUO Ping-yi1, SHI Wei-ping1, ZHOU Mei-xue1, 2 |
1 College of Agronomy, Shanxi Agricultural University, Jinzhong 030801, P.R.China
2 Tasmanian Institute of Agriculture, University of Tasmania, Hobart 7250, Australia
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摘要 YABBY基因是植物种特有的一类转录因子，它在植物生长和发育中发挥着重要作用。为研究YABBY基因在植物抗逆性中的功能，我们分析了谷子（Setaria italica）YABBY基因家族及它们在植物生长和响应不同非生物胁迫的功能。研究结果发现，8个YABBY基因不均等分布在五条染色体上，它们与其他单子叶植物YABBY基因具有很好的共线性关系。系统发育进化关系将SiYAB划分为四个亚组：FIL/YAB3, YAB2, INO和CRC。其中没有单子叶植物YABBY成员被划入YAB5亚组。 此外，在SiYAB蛋白发现有四个保守基序，基序1构成YABBY结构域，而基序2和3构成C2-C2结构域。表达模式分析发现SiYAB基因在生殖发育组织中高表达。为进一步研究SiYAB基因的功能，选择SiDL基因在拟南芥中进行异源表达。SiDL在拟南芥中的过量表达会导致植物开花延迟、叶片卷曲和种子大小发生改变。 此外，SiDL在植物种负向调控对盐胁迫的响应。我们的研究为进一步研究谷子YABBY基因功能提供了基础。
Abstract
YABBY genes are plant-specific transcription factors (TF) that function in plant growth and development. To investigate the functions of the YABBY genes in plants’ stress tolerance, we analyzed the YABBY genes in foxtail millet (Setaria italica) and investigated their functions on plant growth and responses to different stresses. Eight YABBY genes were identified on five chromosomes. These genes showed strong relationships with YABBY genes in other monocot species. Phylogenetical SiYABs were classified into four clades: FIL/YAB3, YAB2, INO, and CRC. No monocot YABBY member was classified into the YAB5 clade. Four conserved motifs were identified and motif 1 constituted the YABBY domain, whereas motifs 2 and 3 formed the C2-C2 region. SiYAB genes were highly expressed in reproductive tissues. Among all the SiYABs, SiDL was selected to be overexpressed in Arabidopsis thaliana to check the functions of the YABBY genes. Overexpression of SiDL in Arabidopsis thaliana caused delayed flowering, leaf curling, and reduced seed size. In addition, SiDL acted as a negative regulator in plant response to salt stress. Our study provides information to assist the study of YABBY gene function in S. italica.
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Received: 13 February 2022
Accepted: 12 April 2022
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This work was supported by grants from the National Key R&D Program of China (2021YFD1901101), the Key Science and Technology Program of Shanxi Province, China (20210140601026) and the Project of Biological Breeding of Shanxi Agricultural University, China (YZGC102).
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| About author: GUO Jie, E-mail: nxgj1115326@sxau.edu.cn; Correspondence SHI Wei-ping, Mobile: +86-18935469769, E-mail: shiwei968@sxau.edu.cn; ZHOU Mei-xue, E-mail: meixue.zhou@utas.edu.au
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Cite this article:
GUO Jie, ZHOU Xu-tao, DAI Ke-li, YUAN Xiang-yang, GUO Ping-yi, SHI Wei-ping, ZHOU Mei-xue.
2022.
Comprehensive analysis of YABBY gene family in foxtail millet (Setaria italica) and functional characterization of SiDL. Journal of Integrative Agriculture, 21(10): 2876-2887.
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