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Journal of Integrative Agriculture  2025, Vol. 24 Issue (7): 2640-2654    DOI: 10.1016/j.jia.2025.05.002
Horticulture Advanced Online Publication | Current Issue | Archive | Adv Search |
The MdMYB306-MdERF114 module promotes tolerance to cadmium by regulating MdATG16 in apple

Yusong Liu1, 2, Yiwei Jia1, Yuhao Li1, Jifa Han1, Qianwei Liu1, Xuewen Li1, Zhijun Zhang1, Chao Li1#, Fengwang Ma1#

1 State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, China

2 College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, China

 Highlights 

MdERF114 enhances cadmium tolerance of apple by increasing autophagy activity. 
MdMYB306 interacts with MdERF114 and positively regulates cadmium tolerance in apple.

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摘要  

镉(Cd)胁迫严重威胁着苹果的生长发育。乙烯反应因子(ERFs)是转录因子(TFs)的主要家族,在镉胁迫抗性中起着关键作用。在这项研究中,我们发现ERF转录因子MdERF114在镉胁迫下被诱导。在苹果(Malus domestica)根系中的过表达MdERF114减少了植物体内镉的积累,增强了植株对镉胁迫的耐受性。酵母单杂交(Y1H)检测、双荧光素酶检测和电泳迁移率变化检测表明,MdERF114直接结合MdATG16的启动子并激活其表达以增加自噬活性,从而提高了对镉胁迫的抵抗力。此外,MdMYB306与MdERF104相互作用,通过促进MdERF114对MdATG16启动子的结合来增强对镉胁迫的抗性。我们的研究结果揭示了MdMYB306-MdERF114-MdATG16影响苹果对镉胁迫抗性的重要机制。



Abstract  

Cadmium (Cd) stress is a serious threat to apple growth and development.  Ethylene response factors (ERFs) are a major family of transcription factors (TFs) that play a key role in the resistance to Cd stress.  In this study, we found that the ERF TF MdERF114 was induced in response to Cd stress.  The overexpression of MdERF114 in apple (Malus domestica) roots reduced the accumulation of Cd in the plants and enhanced their tolerance to Cd stress.  Yeast one-hybrid (Y1H) assays, dual-luciferase assays, and electrophoretic mobility shift assays indicated that MdERF114 directly binds to the promoter of MdATG16 and activates its expression to increase autophagic activity, which leads to higher resistance to Cd stress.  In addition, MdMYB306 interacts with MdERF114 and enhances the resistance to Cd stress by promoting the binding of MdERF114 to the promoter of MdATG16.  Our findings reveal an important mechanism by which MdMYB306-MdERF114-MdATG16 influences the resistance of apple to Cd stress.

Keywords:  apple       MdERF114       MdATG16       MdMYB306       Cd stress       autophagy  
Received: 25 March 2024   Online: 09 May 2025   Accepted: 17 June 2024
Fund: 

This work was supported by the earmarked fund for the China Agriculture Research System (CARS-27), the National Natural Science Foundation of China (31972389) and the Key S&T Special Projects of Shaanxi Province, China (2020zdzx03-01-02).

About author:  #Correspondence Chao Li, E-mail: lc453@163.com; Fengwang Ma, E-mail: fwm64@sina.com

Cite this article: 

Yusong Liu, Yiwei Jia, Yuhao Li, Jifa Han, Qianwei Liu, Xuewen Li, Zhijun Zhang, Chao Li, Fengwang Ma. 2025. The MdMYB306-MdERF114 module promotes tolerance to cadmium by regulating MdATG16 in apple. Journal of Integrative Agriculture, 24(7): 2640-2654.

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