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OsMas1, a novel maspardin protein gene, confers tolerance to salt and drought stresses by regulating ABA signaling in rice
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WANG Fei-bing1*, WAN Chen-zhong1, NIU Hao-fei1, QI Ming-yang1, LI Gang2, ZHANG Fan3, HU Lai-bao1, YE Yu-xiu1, WANG Zun-xin1, PEI Bao-lei, CHEN Xin-hong1, YUAN Cai-yong2
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1 School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an 223003, P.R.China
2 Huaiyin Institute of Agricultural Sciences of Xuhuai Region, Huai’an 223001, P.R.China
3 Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, P.R.China
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摘要 水稻（Oryza sativa L.）是世界主要粮食作物之一，干旱和高盐等非生物胁迫环境严重影响水稻产量，提高水稻水分利用效率的主要途径是提高其抗旱性和耐盐性。克隆和利用水稻抗旱耐盐相关基因，提高水稻的抗逆能力，对我国乃至全世界粮食安全都具有重要意义。本研究报道了水稻maspardin蛋白基因OsMas1的克隆、生物学功能以及分子作用机制。亚细胞定位分析，结果表明 OsMas1蛋白定位于细胞质。逆境胁迫表达分析表明，OsMas1基因受到200 mM甘露醇、20% PEG6000、200 mM NaCl和100 μM ABA诱导表达。构建植物过表达载体和RNAi干扰载体，将该基因导入水稻品种中花11号（WT），通过对转基因水稻材料抗性鉴定，结果表明过表达（OsMas1-OE）植株的耐盐性和抗旱性显著增强，而干扰（OsMas1-RNAi）植株的耐盐性和抗旱性显著降低。对OsMas1-OE、OsMas1-RNAi和WT水稻材料进行芽期和苗期的外源ABA处理，结果发现OsMas1-OE植株对ABA的敏感性明显高于WT植株，而OsMas1-RNAi植株对ABA的敏感性明显低于WT植株。干旱、盐胁迫处理后，OsMas1-OE植株的ABA、脯氨酸、K+含量以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）和光合活性显著高于OsMas1-RNAi植株和WT植株，而丙二醛（MDA）、过氧化氢（H2O2）、超氧阴离子自由基（O2-）和Na+含量显著低于OsMas1-RNAi植株和WT植株。qRT-PCR分析表明，在盐、干旱胁迫下，过表达OsMas1基因显著上调ABA生物合成与信号途径、脯氨酸生物合成途径、活性氧（ROS）清除系统、光合作用和离子转运相关基因的表达，从而显著提高了转基因水稻植株的耐盐性和抗旱性，为水稻水分高效利用和抗逆育种奠定理论基础。
Abstract
Drought and salt stresses, the major environmental abiotic stresses in agriculture worldwide, affect plant growth, crop productivity, and quality. Therefore, developing crops with higher drought and salt tolerance is highly desirable. This study reported the isolation, biological function, and molecular characterization of a novel maspardin gene, OsMas1, from rice. The OsMas1 protein was localized to the cytoplasm. The expression levels of OsMas1 were up-regulated under mannitol, PEG6000, NaCl, and abscisic acid (ABA) treatments in rice. The OsMas1 gene was introduced into the rice cultivar Zhonghua 11 (wild type, WT). OsMas1-overexpression (OsMas1-OE) plants exhibited significantly enhanced salt and drought tolerance; in contrast, OsMas1-interference (OsMas1-RNAi) plants exhibited decreased tolerance to salt and drought stresses, compared with WT. OsMas1-OE plants exhibited enhanced hypersensitivity, while OsMas1-RNAi plants showed less sensitivity to exogenous ABA treatment at both germination and post-germination stages. ABA, proline and K+ contents and superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and photosynthesis activities were significantly increased. In contrast, malonaldehyde (MDA), hydrogen peroxide (H2O2), superoxide anion radical (O2-·), and Na+ contents were significantly decreased in OsMas1-OE plants compared with OsMas1-RNAi and WT plants. Overexpression of OsMas1 up-regulated the genes involved in ABA signaling, proline biosynthesis, reactive oxygen species (ROS)-scavenging system, photosynthesis, and ion transport under salt and drought stresses. Our results indicate that the OsMas1 gene improves salt and drought tolerance in rice, which may serve as a candidate gene for enhancing crop resistance to abiotic stresses.
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Received: 05 November 2021
Accepted: 07 January 2022
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This work was supported by the Natural Science Foundation of Jiangsu Province, China (BK20191483), the Natural Science Fund for Colleges and Universities in Jiangsu Province, China (20KJA180004), the Postgraduate Practice Innovation Program of Jiangsu Province, China (SJCX20_1339), the College Student Practice Innovation Program of Jiangsu Province, China (202111049104H, 202211049133H and 202211049138H) and the Talent Introduction Research Project of Huaiyin Institute of Technology, China (Z301B16534).
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| About author: Correspondence WANG Fei-bing, E-mail: wangfeibing1986@163.com |
Cite this article:
WANG Fei-bing, WAN Chen-zhong, NIU Hao-fei, QI Ming-yang, LI Gang, ZHANG Fan, HU Lai-bao, YE Yu-xiu, WANG Zun-xin, PEI Bao-lei, CHEN Xin-hong, YUAN Cai-yuan.
2023.
OsMas1, a novel maspardin protein gene, confers tolerance to salt and drought stresses by regulating ABA signaling in rice
. Journal of Integrative Agriculture, 22(2): 341-359.
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