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Special Issue:
麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources
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| Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments |
LIU Hang1*, TANG Hua-ping1*, LUO Wei1, MU Yang1, JIANG Qian-tao1, LIU Ya-xi1, CHEN Guo-yue1, WANG Ji-rui1, ZHENG Zhi2, QI Peng-fei1, JIANG Yun-feng1, CUI Fa3, SONG Yin-ming4, YAN Gui-jun5, WEI Yu-ming1, LAN Xiu-jin1, ZHENG You-liang1, MA Jian1 |
1 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
2 Agriculture and Food, CSIRO, St Lucia, Brisbane 4217, Australia
3 College of Agriculture, Ludong University, Yantai 264000, P.R.China
4 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100093, P.R.China
5 School of Plant Biology, The University of Western Australia, Perth WA 6009, Australia |
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摘要
下节间直径（UID）是与小麦穗部发育和丰产性相关的重要形态性状。而我们对其遗传基础的了解知之甚少。本文在5个小麦重组自交系（RIL）群体中利用高密度遗传图谱鉴定了控制UID的数量性状位点（QTL）。在5个RIL群体中共检测到25个UID QTL，分别位于1A、1D（3个QTL）、2B（2）、2D（3）、3B、3D、4A、4B（3）、4D、5A（5）、5B（2）、6B、7D染色体上。其中，5个主效且稳定的QTL：QUid.sau-2CN-1D.1、QUid.sau-2SY-1D、QUid.sau-QZ-2D、QUid.sau-SC-3D和QUid.sau-AS-4B分别在5个RIL群体的多个环境中检测到。QUid.sau-2CN-1D、QUid.sau-2SY-1D与QUid.sau-SC-3D 为三个新的UID 位点。我们进一步开发了与主效QTL紧密连锁的竞争性等位基因特异性PCR（KASP）标记，用于构建近等基因系（NILs）。此外，我们还在主效QTL的物理区间内预测了候选基因，这些候选基因大多与植物发育和水分运输有关。以中国春为参考基因组，我们对定位到的主效QTL的物理区间进行了比较，结果表明，QUid.sau-2CN-1D.1和QUid.sau-2SY-1D可能是等位基因，进一步证实了它们的真实性和有效性。本文也对UID与其他农艺性状的相关关系及UID的大小进行了讨论。总体而言，我们的研究结果剖析了小麦UID的潜在遗传基础，为这些QTL的进一步精细定位和图位克隆奠定了基础。
Abstract Uppermost-internode diameter (UID) is a key morphological trait associated with spike development and yield potential in wheat. Our understanding of its genetic basis remains largely unknown. Here, quantitative trait loci (QTLs) for UID with high-density genetic maps were identified in five wheat recombinant inbred line (RIL) populations. In total, 25 QTLs for UID were detected in five RIL populations, and they were located on chromosomes 1A, 1D (3 QTL), 2B (2), 2D (3), 3B, 3D, 4A, 4B (3), 4D, 5A (5), 5B (2), 6B, and 7D. Of them, five major and stable QTLs (QUid.sau-2CN-1D.1, QUid.sau-2SY-1D, QUid.sau-QZ-2D, QUid.sau-SC-3D, and QUid.sau-AS-4B) were identified from each of the five RIL populations in multiple environments. QUid.sau-2CN-1D.1, QUid.sau-2SY-1D and QUid.sau-SC-3D are novel QTLs. Kompetitive Allele Specific PCR (KASP) markers tightly linked to them were further investigated for developing near-isogenic lines (NILs) carrying the major loci. Furthermore, candidate genes at these intervals harboring major and stable QTLs were predicted, and they were associated with plant development and water transportation in most cases. Comparison of physical locations of the identified QTL on the ‘Chinese Spring’ reference genome showed that several QTLs including two major ones, QUid.sau-2CN-1D.1 and QUid.sau-2SY-1D, are likely allelic confirming their validity and effectiveness. The significant relationships detected between UID and other agronomic traits and a proper UID were discussed. Collectively, our results dissected the underlying genetic basis for UID in wheat and laid a foundation for further fine mapping and map-based cloning of these QTLs.
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Received: 04 June 2020
Accepted:
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| Fund: This work was supported by the projects from the National Natural Science Foundation of China (31971937 and 31970243), the Key Projects of Scientific and Technological Activities for Overseas Students of Sichuan Province, China and the Applied Basic Research Programs of Science and Technology Department of Sichuan Province, China (2020YJ0140). |
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Corresponding Authors:
Correspondence MA Jian, Tel: +86-28-86293115, Fax: +86-28-82650350, E-mail: jianma@sicau.edu.cn
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| About author:
* These authors contributed equally to this study. |
Cite this article:
LIU Hang, TANG Hua-ping, LUO Wei, MU Yang, JIANG Qian-tao, LIU Ya-xi, CHEN Guo-yue, WANG Ji-rui, ZHENG Zhi, QI Peng-fei, JIANG Yun-feng, CUI Fa, SONG Yin-ming, YAN Gui-jun, WEI Yuming, LAN Xiu-jin, ZHENG You-liang, MA Jian.
2021.
Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments. Journal of Integrative Agriculture, 20(11): 2849-2861.
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