Please wait a minute...
Journal of Integrative Agriculture  2023, Vol. 22 Issue (3): 727-737    DOI: 10.1016/j.jia.2022.08.058
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Discrimination of individual seed viability by using the oxygen consumption technique and headspace-gas chromatography-ion mobility spectrometry

TU Ke-ling1, YIN Yu-lin2, YANG Li-ming3, WANG Jian-hua1, SUN Qun1#

1 Department of Seed Science and Biotechnology, College of Agronomy and Biotechnology, China Agricultural University/The Innovation Center (Beijing) of Crop Seeds Whole-Process Technology Research, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement, Beijing 100193, P.R.China

2 College of Arts and Science of Hubei Normal University, Huangshi 435109, P.R.China

3 College of Science, China Agricultural University, Beijing 100083, P.R.China

Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
摘要  鉴定和筛选优质种子对于提高作物产量至关重要。本研究的目的是通过预测单粒种子的潜在发芽能力,判定种子是否具有生活力,以改进作物种子的精选技术,从而提高种子质量。本研究基于种子萌发早期的耗氧量(Q)和种子的挥发性气体成分对单粒种子的生活力进行判定。甜玉米种子、辣椒、小麦种子,有生活力和无生活力种子,其呼吸特征值——最终耗氧量(Q120)均存在差异。在此基础上,为了缩短Q2技术的测量过程,尽可能地减少测量过程中对种子造成不可逆的损害,我们探究了新的耗氧量变量Qt,将辣椒、甜玉米和小麦种子生活力评估的时间分别提前到12、6和9小时,生活力预测的准确率分别为91.9%、97.7%和96.2%。此过程可摒弃无生活力的种子,从而提高种子批的质量,将三种作物的发芽率分别从原始的86.6%、90.9%和53.8%提高到100%。考虑到单粒甜玉米种子的质量较大,存在从单粒种子上检测到挥发性气体成分的可能性,我们进一步尝试了将空气相色谱-离子迁移谱技术(HS-GC-IMS)应用于单粒甜玉米种子的生活力判定,共鉴定出48个峰,其中38种挥发性物质被表征,包括醇、醛、酸和酯。然而,由于单粒甜玉米种子之间挥发性气体成分的差异极为细微,有生活力种子和无生活力种子之间未检测到显著差异,基于挥发性气体成分的主成分分析也无法区分这两种类型的甜玉米种子。本研究所采取的这些方法可为单粒种子生活力的快速鉴定提供参考。

Abstract  

Identifying and selecting high-quality seeds is crucial for improving crop yield.  The purpose of this study was to improve the selection of crop seeds based on separating vital seeds from dead seeds, by predicting the potential germination ability of each seed, and thus improving seed quality.  The methods of oxygen consumption (Q) of seeds and the headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS) were evaluated for identifying the viability of individual seeds.  Firstly, the oxygen consumption technique showed clear differences among the values related to respiratory characteristics for seeds that were either vital or not, and the discrimination ability of final oxygen consumption (Q120) was achieved not only in sweet corn seeds but also in pepper and wheat seeds.  Besides, Qt was established as a new variable to shorten the measuring process in the Q2 (oxygen sensor) procedure, which was significantly related to the viability of individual seeds.  To minimize seed damage during measurement, the timing for viability evaluation was pinpointed at the 12, 6 and 9 h for pepper, sweet corn, and wheat seeds based on the new variables concerning oxygen consumption (i.e., Q12, Q6 and Q9, respectively).  The accuracies of viability prediction were 91.9, 97.7 and 96.2%, respectively.  Dead seeds were identified and hence discarded, leading to an enhancement in the quality of the seed lot as indicated by an increase in germination percentage, from 86.6, 90.9, and 53.8% to all at 100%.  We then used the HS-GC-IMS to determine the viability of individual sweet corn seeds, noting that corn seed has a heavier weight so the volatile gas components are more likely to be detected.  A total of 48 chromatographic peaks were identified, among which 38 target compounds were characterized, including alcohols, aldehydes, acids and esters.  However, there were no significant differences between the vital and dead seeds, due to the trace amount volatile composition differences among the individual seeds.  Furthermore, a PCA based on the signal intensities of the target volatile compounds obtained was found to lose its effectiveness, as it was unable to distinguish those two types of sweet corn seeds.  These strategies can provide a reference for the rapid detection of single seed viability.

Keywords:  headspace-gas chromatography-ion mobility spectrometry        oxygen consumption        seed respiration        sweet corn        tomato        wheat  
Received: 13 September 2021   Accepted: 10 November 2021
Fund: This work was supported by the National Key Research and Development Program of China (2018YFD0100903).
About author:  TU Ke-ling, E-mail: b20193010029@cau.edu.cn; #Correspondence SUN Qun, E-mail: sunqun@cau.edu.cn, sqcau@126.com

Cite this article: 

TU Ke-ling, YIN Yu-lin, YANG Li-ming, WANG Jian-hua, SUN Qun. 2023. Discrimination of individual seed viability by using the oxygen consumption technique and headspace-gas chromatography-ion mobility spectrometry. Journal of Integrative Agriculture, 22(3): 727-737.

Alani A, Bruzau F, Raymond P, Saintges V, Leblanc J M, Pradet A. 1985. Germination, respiration, and adenylate energy-charge of seeds at various oxygen partial pressures. Plant Physiology, 79, 885–890.
Arroyo-Manzanares N, Martin-Gomez A, Jurado-Campos N, Garrido-Delgado R, Arce C, Arce L. 2018. Target vs spectral fingerprint data analysis of iberian ham samples for avoiding labelling fraud using headspace-gas chromatography-ion mobility spectrometry. Food Chemistry, 246, 65–73.
Baalbaki R Z, Elias S G, Filho J M, McDonald M B. 2009. Seed Vigor Testing Handbook. Association of Official Seed Analysis, Ithaca, New York.
Bello P, Bradford K J. 2016. Single-seed oxygen consumption measurements and population-based threshold models link respiration and germination rates under diverse conditions. Seed Science Research, 26, 199–221.
Bello P, Bradford K J. 2021. Relationships of brassica seed physical characteristics with germination performance and plant blindness. Agriculture-Basel, 11, 220.
Bewley J D, Bradford K J, Hilhorst H, Nonogaki H. 2013. Dormancy and the control of germination. Seeds. Springer, New York, NY. 
Bradford K J, Bello P, Fu J C, Barros M. 2013. Single-seed respiration: A new method to assess seed quality. Seed Science and Technology, 41, 420–438.
Bradford K J, Benech-Arnold R L, Come D, Corbineau F. 2008. Quantifying the sensitivity of barley seed germination to oxygen, abscisic acid, and gibberellin using a population-based threshold model. Journal of Experimental Botany, 59, 335–347.
Bradford K J, Come D, Corbineau F. 2007. Quantifying the oxygen sensitivity of seed germination using a population-based threshold model. Seed Science Research, 17, 33–43.
Chen N F, Zhu Z J, He Y, Zhao G W, Shi H X. 2010. Applying Q2 technology to quickly test and improve tomato seed vigor. China Vegetables, 20, 47–51. (in Chinese)
Chen R J, Zhao G W, Cao D D, Ruan G H, Chen H Y. 2013. The application of oxygen sensing technology in rapid vigor testing of treated conventional rice seeds. Seed, 32, 8–11, 16. (in Chinese)
Colville L, Bradley E L, Lloyd A S, Pritchard H W, Castle L, Kranner I. 2012. Volatile fingerprints of seeds of four species indicate the involvement of alcoholic fermentation, lipid peroxidation, and maillard reactions in seed deterioration during ageing and desiccation stress. Journal of Experimental Botany, 63, 6519–6530.
Elias S G, Kopeland L O, McDonald M S, Baalbaki R Z. 2012. Seed Testing Principles and Practices. East Lansing, Michigan, Michigan State University Press, USA.
Ge S, Chen Y Y, Ding S H, Zhou H, Jiang L W, Yi Y J, Deng F M, Wang R R. 2020. Changes in volatile flavor compounds of peppers during hot air drying process based on headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS). Journal of the Science of Food and Agriculture, 100, 3087–3098.
He Y, Ye Z X, Ying Q S, Ma Y P, Zang Y X, Wang H S, Yu Y J, Zhu Z J. 2019. Glyoxylate cycle and reactive oxygen species metabolism are involved in the improvement of seed vigor in watermelon by exogenous GA3. Scientia Horticulturae, 247, 184–194.
Hourmant A, Pradet A. 1981. Oxidative-phosphorylation in germinating lettuce seeds (Lactuca sativa) during the 1st hours of imbibition. Plant Physiology, 68, 631–635.
Karpas Z. 2013. Applications of ion mobility spectrometry (IMS) in the field of food omics. Food Research International, 54, 1146–1151.
Liu D Y, Bai L, Feng X, Chen Y P, Zhang D N, Yao W S, Zhang H, Chen G L, Liu Y. 2020. Characterization of Jinhua ham aroma profiles in specific to aging time by gas chromatography-ion mobility spectrometry (GC-IMS). Meat Science, 168, 108178.
Liu Y J, Bu M T, Gong X, He J N, Zhan Y. 2021. Characterization of the volatile organic compounds produced from avocado during ripening by gas chromatography ion mobility spectrometry. Journal of the Science of Food and Agriculture, 101, 666–672.
Ma T, Tsuchikawa S, Inagaki T. 2020. Rapid and non-destructive seed viability prediction using near-infrared hyperspectral imaging coupled with a deep learning approach. Computers and Electronics in Agriculture, 177, 105683.
Michalak M, Plitta-Michalak B P, Nadarajan J, Colville L. 2021. Volatile signature indicates viability of dormant orthodox seeds. Physiologia Plantarum, 173, 788–804.
Mira S, Gonzalez-Benito M E, Hill L M, Walters C. 2010. Characterization of volatile production during storage of lettuce (Lactuca sativa) seed. Journal of Experimental Botany, 61, 3915–3924.
Patane C, Cavallaro V, Avola G, D’Agosta G. 2006. Seed respiration of sorghum [Sorghum bicolor (L.) Moench] during germination as affected by temperature and osmoconditioning. Seed Science Research, 16, 251–260.
Qu D Y. 2003. History and status of the vegetable industry in China. Asian Plants with Unique Horticultural Potential: Genetic Resources, Cultural Practices, and Utilization. pp. 43–51.
Thu-Phuong N, Cueff G, Hegedus D D, Rajjou L, Bentsink L. 2015. A role for seed storage proteins in Arabidopsis seed longevity. Journal of Experimental Botany, 66, 6399–6413.
Tu K L, Li L J, Yang L M, Wang J H, Sun Q. 2018. Selection for high quality pepper seeds by machine vision and classifiers. Journal of Integrative Agriculture, 17, 1999–2006.
Wang Y C, Liang S Q, Wang Y H, Zhao G W, Cao D D. 2016. Effect of harvest time on seed vigor and respiratory metabolism of waxy maize seeds. Acta Agriculturae Zhejiangensis, 28, 910–914. (in Chinese)
Wei S Y. 2014. Quickly detecting of tomato coating seeds qualities by Q2. Seed, 33, 110–113. (in Chinese)
Zhang T T, Ayed C, Fisk I D, Pan T, Wang J H, Yang N, Sun Q. 2021. Evaluation of volatile metabolites as potential markers to predict naturally-aged seed vigour by coupling rapid analytical profiling techniques with chemometrics. Food Chemistry, 367, 130760.
Zhang X X, Dai Z, Fan X J, Liu M, Ma J F, Shang W T, Liu J, Strappe P, Blanchard C, Zhou Z. 2020. A study on volatile metabolites screening by hs-spme-gc-ms and hs-gc-ims for discrimination and characterization of white and yellowed rice. Cereal Chemistry, 97, 496–504.
Zhao G W, Zhong T L. 2012. Improving the assessment method of seed vigor in Cunninghamia lanceolata and Pinus massoniana based on oxygen sensing technology. Journal of Forestry Research, 23, 95–101. 
[1] Ping’an Zhang, Mo Li, Qiang Fu, Vijay P. Singh, Changzheng Du, Dong Liu, Tianxiao Li, Aizheng Yang.

Dynamic regulation of the irrigation–nitrogen–biochar nexus for the synergy of yield, quality, carbon emission and resource use efficiency in tomato [J]. >Journal of Integrative Agriculture, 2024, 23(2): 680-697.

[2] Changqin Yang, Xiaojing Wang, Jianan Li, Guowei Zhang, Hongmei Shu, Wei Hu, Huanyong Han, Ruixian Liu, Zichun Guo.

Straw return increases crop production by improving soil organic carbon sequestration and soil aggregation in a long-term wheat–cotton cropping system [J]. >Journal of Integrative Agriculture, 2024, 23(2): 669-679.

[3] DU Dan, HU Xin, SONG Xiao-mei, XIA Xiao-jiao, SUN Zhen-yu, LANG Min, PAN Yang-lu, ZHENG Yu, PAN Yu. SlTPP4 participates in ABA-mediated salt tolerance by enhancing root architecture in tomato[J]. >Journal of Integrative Agriculture, 2023, 22(8): 2384-2396.
[4] HU Wen-jing, FU Lu-ping, GAO De-rong, LI Dong-sheng, LIAO Sen, LU Cheng-bin. Marker-assisted selection to pyramid Fusarium head blight resistance loci Fhb1 and Fhb2 in a high-quality soft wheat cultivar Yangmai 15[J]. >Journal of Integrative Agriculture, 2023, 22(2): 360-370.
[5] ZHANG Guang-xin, ZHAO De-hao, FAN Heng-zhi, LIU Shi-ju, LIAO Yun-cheng, HAN Juan. Combining controlled-release urea and normal urea with appropriate nitrogen application rate to reduce wheat stem lodging risk and increase grain yield and yield stability[J]. >Journal of Integrative Agriculture, 2023, 22(10): 3006-3021.
[6] HUANG Feng, LI Xuan-shuang, DU Xiao-yu, LI Shun-cheng, LI Nan-nan, LÜ Yong-jun, ZOU Shao-kui, ZHANG Qian, WANG Li-na, NI Zhong-fu, HAN Yu-lin, XING Jie-wen. SNP-based identification of QTLs for thousand-grain weight and related traits in wheat 8762/Keyi 5214 DH lines[J]. >Journal of Integrative Agriculture, 2023, 22(10): 2949-2960.
[7] Carlos Kwesi TETTEY, YAN Zhi-yong, MA Hua-yu, ZHAO Mei-sheng, GENG Chao, TIAN Yan-ping, LI Xiang-dong . Tomato mottle mosaic virus: characterization, resistance gene effectiveness, and quintuplex RT-PCR detection system[J]. >Journal of Integrative Agriculture, 2022, 21(9): 2641-2651.
[8] LI Si-ping, ZENG Lu-sheng, SU Zhong-liang. Wheat growth, photosynthesis and physiological characteristics under different soil Zn levels[J]. >Journal of Integrative Agriculture, 2022, 21(7): 1927-1940.
[9] ZHANG Hai-feng, Tofazzal ISLAM, LIU Wen-de. Integrated pest management programme for cereal blast fungus Magnaporthe oryza[J]. >Journal of Integrative Agriculture, 2022, 21(12): 3420-3433.
[10] ZHAO Lai-bin, XIE Die, HUANG Lei, ZHANG Shu-jie, LUO Jiang-tao, JIANG Bo, NING Shun-zong, ZHANG Lian-quan, YUAN Zhong-wei, WANG Ji-rui, ZHENG You-liang, LIU Deng-cai, HAO Ming. Integrating the physical and genetic map of bread wheat facilitates the detection of chromosomal rearrangements[J]. >Journal of Integrative Agriculture, 2021, 20(9): 2333-2342.
[11] Hakan FIDAN, Pelin SARIKAYA, Kubra YILDIZ, Bengi TOPKAYA, Gozde ERKIS, Ozer CALIS. Robust molecular detection of the new Tomato brown rugose fruit virus in infected tomato and pepper plants from Turkey[J]. >Journal of Integrative Agriculture, 2021, 20(8): 2170-2179.
[12] YAN Zhi-yong, ZHAO Mei-sheng, MA Hua-yu, LIU Ling-zhi, YANG Guang-ling, GENG Chao, TIAN Yan-ping, LI Xiang-dong. Biological and molecular characterization of tomato brown rugose fruit virus and development of quadruplex RT-PCR detection[J]. >Journal of Integrative Agriculture, 2021, 20(7): 1871-1879.
[13] LI Si-nan, CHEN Wen, MA Xin-yao, TIAN Xia-xia, LIU Yao, HUANG Li-li, KANG Zhen-sheng, ZHAO Jie. Identification of eight Berberis species from the Yunnan-Guizhou plateau as aecial hosts for Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen[J]. >Journal of Integrative Agriculture, 2021, 20(6): 1563-1569.
[14] LIU Yang, LI Yu-xiang, LI Yi-xiang, TIAN Zhong-wei, HU Jin-ling, Steve ADKINS, DAI Ting-bo. Changes of oxidative metabolism in the roots of wheat (Triticum aestivum L.) seedlings in response to elevated ammonium concentrations[J]. >Journal of Integrative Agriculture, 2021, 20(5): 1216-1228.
[15] WU Li-hong, ZHOU Cao, LONG Gui-yun, YANG Xi-bin, WEI Zhi-yan, LIAO Ying-jiang, YANG Hong, HU Chao-xing . Fitness of fall armyworm, Spodoptera frugiperda to three solanaceous vegetables[J]. >Journal of Integrative Agriculture, 2021, 20(3): 755-763.
No Suggested Reading articles found!