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Special Issue:
麦类遗传育种合辑Triticeae Crops Genetics · Breeding · Germplasm Resources
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| Quantitative trait loci analysis for root traits in synthetic hexaploid wheat under drought stress conditions |
| LIU Rui-xuan1, WU Fang-kun1, YI Xin2, LIN Yu1, WANG Zhi-qiang1, LIU Shi-hang1, DENG Mei1, MA Jian1, WEI Yu-ming1, ZHENG You-liang1, LIU Ya-xi1
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1 Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
2 College of Environmental Sciences, Sichuan Agricultural University, Chengdu 611130, P.R.China |
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Abstract Synthetic hexaploid wheat (SHW), possesses numerous genes for drought that can help breeding for drought-tolerant wheat varieties. We evaluated 10 root traits at seedling stage in 111 F9 recombinant inbred lines derived from a F2 population of a SHW line (SHW-L1) and a common wheat line, under normal (NC) and polyethylene glycol-simulated drought stress conditions (DC). We mapped quantitative trait loci (QTLs) for root traits using an enriched high-density genetic map containing 120 370 single nucleotide polymorphisms (SNPs), 733 diversity arrays technology markers (DArT) and 119 simple sequence repeats (SSRs). With four replicates per treatment, we identified 19 QTLs for root traits under NC and DC, and 12 of them could be consistently detected with three or four replicates. Two novel QTLs for root fresh weight and root diameter under NC explained 9 and 15.7% of the phenotypic variation respectively, and six novel QTLs for root fresh weight, the ratio of root water loss, total root surface area, number of root tips, and number of root forks under DC explained 8.5–14% of the phenotypic variation. Here seven of eight novel QTLs could be consistently detected with more than three replicates. Results provide essential information for fine-mapping QTLs related to drought tolerance that will facilitate breeding drought-tolerant wheat cultivars.
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Received: 16 April 2019
Accepted:
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| Fund: This study was supported by the National Natural Science Foundation of China (31771794, 91731305 and 31560388), the outstanding Youth Foundation of the Department of Science and Technology of Sichuan Province, China (2016JQ0040), the Key Technology Research and Development Program of the Department of Science and Technology of Sichuan Province, China (2016NZ0057), and the International Science & Technology Cooperation Program of the Bureau of Science and Technology of Chengdu, China (2015DFA306002015-GH03-00008-HZ). |
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Corresponding Authors:
Correspondence LIU Ya-xi, Tel: +86-28-86290952, Fax: +86-28-82650350, E-mail: liuyaxi@sicau.edu.cn, yaxi.liu@hotmail.com, ORCID: 0000-0001-6814-7218
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| About author: LIU Rui-xuan, E-mail: 947130299@qq.com; |
Cite this article:
LIU Rui-xuan, WU Fang-kun, YI Xin, LIN Yu, WANG Zhi-qiang, LIU Shi-hang, DENG Mei, MA Jian, WEI Yu-ming, ZHENG You-liang, LIU Ya-xi.
2020.
Quantitative trait loci analysis for root traits in synthetic hexaploid wheat under drought stress conditions. Journal of Integrative Agriculture, 19(8): 1947-1960.
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