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Journal of Integrative Agriculture
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Grape DNA methyltransferase VvMET2b overexpression increases genome-wide methylation and enhances seed salt tolerance

Fengxia Wang1, Qinghua Liu3, Ao Li1, Zhanfeng Xu4, Guangqing Xiang1, Huiping Liu1, Qingtian Zhang1, Ke Li1, Qian Mu1, Yuxin Yao5, Zhichang Zhang2, Pengfei Wang1#

Shandong Academy of Grape, Shandong Academy of Agricultural Sciences, Jinan 250100, China

Shandong Zhichang Agricultural Science and Technology Development Co., Ltd., Rizhao 276500, China

College of Biological Science and Technology, Jinan University, Jinan 250000, China

College of Enology and Horticulture, Ningxia University, Ningxia 750021, China

College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An 271018, China

 Highlights 

VvMET2b enhanced seed salt tolerance

VvMET2b increased DNA methylation levels under salt stress

VvMET2b modulated salt tolerance through DNA methylation

VvMET2b modulated the expression of transcription factors and hormone-related genes 

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摘要  

MET1是一类编码DNA甲基转移酶的基因,是非生物胁迫响应与适应过程中的主要调节因子。为了探究DNA甲基转移酶1在盐胁迫中的作用,我们克隆了一个葡萄(Vitis vinifera L.MET1家族基因VvMET2b,并将其在拟南芥中过表达。转基因拟南芥表型分析表明,VvMET2b在盐处理下提高了种子萌发率和幼苗存活率。详细的甲基组分析发现VvMET2b 提高了转基因株系的整体甲基化水平,改变了差异甲基化区域(DMRs)的数量和甲基化类型。转录组分析表明,盐胁迫下,许多转录因子如NACsMYBsWRKYsVvMET2bOE转基因株系中发生了差异表达。盐胁迫下,过表达VvMET2b能够诱导细胞分裂素负调节因子AARRs、跨膜转运蛋白KAT1的上调表达,抑制MYB6的表达。同时,VvMET2b也减弱了对生长素相关基因 Aux/IAAsGH3s、扩展蛋白EXPA17、水通道蛋白TIP2 的调控作用。VvMET2b通过改变MYB6TIP2EXPA17的甲基化水平调控这些基因的表达。综上所述,VvMET2b可能通过DNA甲基化的变化和某些关键基因的表达提高种子的耐盐性。



Abstract  

MET1 encodes DNA methyltransferase 1, which is increasingly being identified as a major regulator of abiotic stress responses and adaptation. To explore the function of DNA methyltransferase 1 in salt stress, we cloned a grape (Vitis vinifera L.) MET1 subfamily gene, VvMET2b, and overexpressed it into A. thaliana. The phenotypic analysis of transgenic Arabidopsis revealed that VvMET2b improved seed germination and seedling survival under NaCl treatment. Detailed methylome analysis revealed that VvMET2b increased the global methylation level of transgenic plants and altered the quantity of differentially methylated regions (DMRs) and DNA methylation types. Comprehensive transcriptome analyses indicated that many transcription factors, such as NACs, MYBs, and WRKYs were differentially expressed in VvMET2bOE plants under salt stress. VvMET2b overexpression induced the expression of cytokinin negative regulator type-A ARRs, the transmembrane transporter KAT1, inhibited the expression of MYB6, and the up-regulated expression of auxin-related genes Aux/IAAs and down-regulated expression of GH3s, expansin EXPA17 and tonoplast aquaporin TIP2 were mitigated. VvMET2b altered DNA methylation level of MYB6, TIP2 and EXPA17 and thereby may regulate the expression of these genes. Taken together, VvMET2b may regulate seed salt tolerance through DNA methylation changes and certain key gene expression. 

Keywords:  Vitis vinifera L.       VvMET2b              Seed germination              Salt stress              Methylome              Transcriptome  
Online: 18 February 2026  
Fund: 

This work was supported by Improved Variety Program of Shandong Province (2022LZGCQY019), projects ZR2023QC217, ZR2023QC239 and ZR2023QC249 supported by Shandong Provincial Natural Science Foundation; Shandong Academy of Agricultural Sciences, Introduction and Training of High-level Talents (CXGC2025F15); Scientific Research Guide Foundation of Shandong Academy of Grape (SDAG2021A01); Innovation Project of Shandong Academy of Agricultural Sciences (CXGC2025A06); Agriculture key core technology research project of Ningxia (NXNYKJ202304); Jinan Agriculture and Rural Bureau Modern Seed Industry Innovation Development Project - Grape Core Germplasm Protection and Utilization and Gene Bank Construction. 

About author:  *Correspondence Pengfei Wang, E-mail: fengqiaoyouzi@126.com

Cite this article: 

Fengxia Wang, Qinghua Liu, Ao Li, Zhanfeng Xu, Guangqing Xiang, Huiping Liu, Qingtian Zhang, Ke Li, Qian Mu, Yuxin Yao, Zhichang Zhang, Pengfei Wang. 2026. Grape DNA methyltransferase VvMET2b overexpression increases genome-wide methylation and enhances seed salt tolerance. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2026.02.032

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