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Journal of Integrative Agriculture
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A flavonoid 3’-hydroxylase regulates pink tuber skin in potato

Hui Du, 2, 3*, Jin Pu1, 2*, Rongyan Wang1*, Yanhui Zhu2, Pei Wang2, Zhong Zhang2, Guangtao Zhu1#, Chunzhi Zhang2#

1 School of Life Sciences, Yunnan Key Laboratory of Potato Biology, The AGISCAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal University, Southwest United Graduate School, Kunming 650500, China

2 State Key Laboratory of Genome and Multi-omics Technologies, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China

3 School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou 310053, China

 Highlights 

The Pink locus, responsible for pink tuber skin in potato, was fine-mapped to chromosome 3, and the anthocyanin biosynthesis gene StF3′H was identified as the causal gene.

Promoter variations in StF3′H regulate its expression and determine pink pigmentation, and the Red (R) locus acts epistatically over the Pink locus.

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摘要  

马铃薯块茎呈现多种颜色,这主要源于花青素色素的不同积累水平。尽管调控红色和紫色色素积累的基因位点已有报道,但马铃薯中粉色花青素生物合成的调控机制仍不清楚。本研究利用一个BC1S1群体(其块茎薯皮颜色在红色、粉色和黄色之间分离),通过集群分离分析测序方法,鉴定出粉色薯皮位点Pink。我们将Pink的位置缩小到第3号染色体上一个265 kb的区间内。代谢组学和转录组学分析显示,花青素合成基因——类黄酮3'-羟化酶(StF3'H)是候选基因。遗传转化试验证明StF3'H是产生粉色薯皮块茎所必需的。此外,我们发现红色薯皮位点(R)Pink具有上位性。这些发现为通过分子育种开发有色马铃薯提供了新的理论基础,并为探索马铃薯花青素生物合成的复杂调控机制提供了重要参考。



Abstract  

The tubers of potato (Solanum tuberosum L.) exhibit diverse colors, primarily resulting from different levels of anthocyanin pigments. While the loci regulating the accumulation of red and purple pigments have been reported, the regulatory mechanism underlying the biosynthesis of pink anthocyanins in potato remains unclear. In this study, we identified the pink tuber skin locus Pink through a bulked-segregant analysis sequencing approach using a BC1S1 population segregating for tubers with red, pink, or yellow skin. We narrowed down the location of Pink to a 265-kb interval on chromosome 3. Metabolomic and transcriptomic analyses revealed the anthocyanin biosynthesis gene Flavonoid 3'-hydroxylase (StF3'H) as the candidate gene. Genetic transformation assays demonstrated that StF3'H is essential for the production of tubers with pink skin. Furthermore, we showed that the red tuber skin locus (R) is epistatic to Pink. These findings provide a new theoretical basis for the development of colored potatoes through molecular breeding and offer an important reference for exploring the complex regulatory mechanisms of anthocyanin biosynthesis in potatoes.

Keywords:  anthocyanins       F3'H              genetic mapping              tuber skin              potato  
Online: 17 February 2026  
Fund: 

The work was supported by Guangdong Major Project of Basic and Applied Basic Research (2021B0301030004), the National Natural Science Foundation of China (32488302 and 32372695), and the Agricultural Science and Technology Innovation Program (CAAS-ZDRW202404). 

Cite this article: 

Hui Du, Jin Pu, Rongyan Wang, Yanhui Zhu, Pei Wang, Zhong Zhang, Guangtao Zhu, Chunzhi Zhang. 2026. A flavonoid 3’-hydroxylase regulates pink tuber skin in potato. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2026.02.029

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