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Journal of Integrative Agriculture
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StCOMT1 enhances potato resistance to Fusarium sporotrichioides by regulating coumarin-related substance accumulation and the ROS system

Jiaqi Wang1,2*, Biao Zhao1,2*, Dan Liu1, Fumeng He1, Chong Du1, Yunzhu Che1, Zengli Zhang1, Xu Feng1, Xue Wang3 ,Yingnan Wang1#, Fenglan Li1,2,3# 

1 College of Life Sciences, Northeast Agricultural University, Harbin 150030, China

2 Heilongjiang High Quality Agricultural Functional Microbial Agent Engineering and Technology Research Centre, Harbin 150030, China

3 Heilongjiang Green Food Science Research Institute, Harbin 150028, China

 Highlights 

l Overexpression of StCOMT1 enhances potato resistance to Fusarium sporotrichioides.

StCOMT1 overexpression leads to a reduction in reactive oxygen species (ROS) accumulation during pathogen infection.

StCOMT1 overexpression promotes the accumulation of coumarin-related compounds in potato.

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摘要  

咖啡酸-O-甲基转移酶COMT)是苯丙烷代谢途径中的关键酶,在木质素与香豆素代谢途径都发挥着重要作用COMT在植物抗病方面的功能已在多个物种得到证实。本研究在全基因组水平铃薯COMT基因家族进行了鉴定,通过系统发育分析结合前期代谢差异、加权基因共表达网络分析WGCNA,确定了StCOMT1脱氧雪腐镰刀菌烯醇(DON)诱导下增强马铃薯抗病性增强候选基因。为了更好地了解StCOMT1基因的功能,我们进行了StCOMT1异源表达与过表达StCOMT1定位于叶绿体中,异源表达发现它可以催化底物的甲基化生成阿魏酸与褪黑素。生理指标显示相较于野生型马铃薯植株,StCOMT1过表达植株受到拟枝孢镰刀菌感染斑面积更小,活性氧(ROS水平降低。基于高效液相色谱HPLC表达谱与RT-qPCR数据的分析发现,接菌后,在StCOMT1过表达植株中香豆素相关化合物与香豆素相关基因呈现器官差异积累与表达。结果表明StCOMT1过表达马铃薯通过增强活性氧清除,促进香豆素相关化合物器官特异性积累,从而增强对拟枝孢镰刀菌的抗性。



Abstract  

Caffeic acid-O-methyltransferase (COMT) is a crucial enzyme in the phenylpropanoid metabolic pathway, with significant roles in both the lignin and coumarin pathways. The function of COMT in plant disease resistance has been demonstrated in several species. Our research identified the potato COMT gene family on a genome-wide scale and identified StCOMT1 as a candidate gene for enhancing potato disease resistance under DON induction through phylogenetic analyses combined with previously identified metabolic differences and weighted gene co-expression network analysis (WGCNA). In order to better understand the function of StCOMT1, heterologous expression and overexpression were conducted. StCOMT1 is localized in chloroplasts and was found to catalyze the methylation of substrates to produce ferulic acid and melatonin in vitro. Physiological parameters showed that, compared with wild-type potato plants, StCOMT1-overexpressing plants infectced with Fusarium sporotrichioides exhibited smaller lesion areas and lower reactive oxygen species (ROS) levels. Based on the analysis of high-performance liquid chromatography (HPLC) expression profiles and RT-qPCR data, it was found that coumarin-related compounds and coumarin-related genes showed organ-differential accumulation and expression in StCOMT1-overexpressing plants after inoculation. The results indicate that StCOMT1 overexpression in potatoes enhanced resistance to F. sporotrichioides by enhancing reactive oxygen species clearance and promoting organ-specific accumulation of coumarin-related compounds.

Keywords:  potato       COMT gene        overexpression        disease resistance        ROS scavenging  
Online: 15 November 2025  
Fund: 

This work was supported by the National Natural Science Foundation of China, grant number U22A20443; Heilongjiang Provincial Research Institutes Scientific Research Operating Expenses Project, grant number CZKYF2023-1-B020; Heilongjiang Province Key Research and Development Plan project, grant number GA23B015. National Natural Science Foundation of China Youth Fund, grant number 32201717.

Cite this article: 

Jiaqi Wang, Biao Zhao, Dan Liu, Fumeng He, Chong Du, Yunzhu Che, Zengli Zhang, Xu Feng, Xue Wang, Yingnan Wang, Fenglan Li. 2025. StCOMT1 enhances potato resistance to Fusarium sporotrichioides by regulating coumarin-related substance accumulation and the ROS system. Journal of Integrative Agriculture, Doi:10.1016/j.jia.2025.11.021

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