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Special Issue:
食品科学合辑Food Science
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| Comparison of structural and physicochemical properties of potato protein and potato flour modified with tyrosinase |
| ZHU Yu1, 2, YUAN Yu-han3, MEI Li-ping1, DING Shuang-kun1, GAO Yu-chen1, DU Xian-feng1, GUO Li2 |
1 Anhui Engineering Laboratory of Agro-products Processing, Anhui Agricultural University, Hefei 230036, P.R.China
2 State Key Laboratory of Biobased Material and Green Papermaking, School of Food Sciences and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, P.R.China
3 College of Life Science and Technology, Xinjiang University, Urumchi 830046, P.R.China
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摘要
本研究中，使用酪氨酸酶对马铃薯蛋白和马铃薯粉进行改性，以促进马铃薯主食的多样化。结果表明，酪氨酸酶处理显著改变了蛋白质的二级结构。酪氨酸酶处理后，马铃薯蛋白质和马铃薯粉的最高分解温度分别从322.32°C上升到332.40°C,从294.24°C上升到299.61°C。酪氨酸酶处理显著降低了马铃薯粉的糊化粘度，即峰值粘度、谷底粘度、衰减值、最终粘度和回生值分别降低了32.50%、60.98%、13.04%、68.24%和74.31%。相反，酪氨酸酶处理增加了蛋白质和面粉凝胶的抗剪切性和硬度；蛋白质和面粉凝胶的最大应力值分别从1.48%增加到10.1%以及6.87%增加到14.8%。此外，酪氨酸酶处理促进了马铃薯蛋白和马铃薯粉的粘弹性和结构稳定性。研究结果为新型马铃薯主食的开发提供了重要的理论依据。
Abstract The present study modified potato protein and flour with tyrosinase to promote the diversification of potato staple foods. The results indicated that tyrosinase treatment markedly altered the secondary structure of proteins. After tyrosinase treatment, the maximum decomposition temperature of potato protein and flour increased from 322.32 to 332.40°C and from 294.24 to 299.61°C, respectively. Tyrosinase treatment remarkably reduced the pasting viscosity of potato flour, that is, the peak viscosity, through reducing viscosity, breakdown, final viscosity, and setback by 32.50, 60.98, 13.04, 68.24, and 74.31%, respectively. In contrast, tyrosinase treatment increased the shear resistance and hardness of the protein and flour gels; the maximum stress values of the protein and flour gels increased from 1.48 to 10.1% and from 6.87 to 14.8%, respectively. Furthermore, tyrosinase treatment promoted viscoelastic properties and structural stability of potato protein and flour. These results may provide an important foundation for the development of novel potato staple foods.
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Received: 06 May 2021
Accepted: 21 October 2021
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| Fund: This research was supported by the National Natural Science Foundation of China (31771933 and 31471700), the Shandong Key Research and Development Plan (Public Welfare Projects), China (2019GSF109035), the International Cooperation Foundation of Qilu University of Technology (Shandong Academy of Sciences), China (QLUTGJUZ014), the Special Funds for Taishan Scholars Project, China (ts201712060), and the Independent Training Innovation Team Project of China (2018GXRC004). |
| About author: ZHU Yu, E-mail: zyzhuyu.good@163.com; Correspondence DU Xian-feng, E-mail: dxf@ahau.edu.cn; GUO Li, E-mail: guolizhuyuer@163.com |
Cite this article:
ZHU Yu, YUAN Yu-han, MEI Li-ping, DING Shuang-kun, GAO Yu-chen, DU Xian-feng, GUO Li.
2022.
Comparison of structural and physicochemical properties of potato protein and potato flour modified with tyrosinase. Journal of Integrative Agriculture, 21(5): 1513-1524.
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Aguilera J M, Baffico P. 1997. Structure-mechanical properties of heat induced whey protein/cassava starch gels. Journal of Food Science, 62, 1048–1066.
Ahmad M, Hani N, Nirmal N, Fazial F F, Mohtar N F, Romli S R. 2015. Optical and thermo-mechanical properties of composite films based on fish gelatin/rice flour fabricated by casting technique. Progress in Organic Coatings, 84, 115–127.
Bártová V, Bárta J, Brabcová A, Zdráhal Z, Horáčková V. 2015. Amino acid composition and nutritional value of four cultivated South American potato species. Journal of Food Composition and Analysis, 40, 78–85.
Chen B, Zhang B, Li M, Xie Y, Chen H. 2018. Effects of glutenin and gliadin modified by protein-glutaminase on pasting, rheological properties and microstructure of potato starch. Food Chemistry, 253, 148–155.
Cummings J H, Beatty E R, Kingman S M, Bingham S A, Englyst H N. 1996. Digestion and physiological properties of resistant starch in the human large bowel. British Journal of Nutrition, 75, 733–747.
Glusac J, Davidesko-Vardi I, Isaschar-Ovdat S, Kukavica B, Fishman A. 2018. Gel-like emulsions stabilized by tyrosinase-crosslinked potato and zein proteins. Food Hydrocolloids, 82, 53–63.
Goldfeder M, Kanteev M, Isaschar-Ovdat S, Adir N, Fishman A. 2014. Determination of tyrosinase substrate-binding modes reveals mechanistic differences between type-3 copper proteins. Nature Communications, 5, 4505.
Goo Y, Kim T, Lee M, Lee S. 2013. Accumulation of PrLeg, a perilla legumin protein in potato tuber results in enhanced level of sulphur-containing amino acids. Comptes Rendus Biologies, 336, 433–439.
Guerrieri N, Eynard L, Lavelli V, Cerletti P. 1997. Interactions of protein and starch studied through amyloglucosidase action. Cereal Chemistry, 74, 846–850.
Gui Y F, Li J H, Zhu Y, Guo L. 2020. Roles of four enzyme crosslinks on structural, thermal and gel properties of potato proteins. LWT-Food Science and Technology, 123, 109–116.
Gui Y F, Zou F X, Li J H, Zhu Y, Guo L, Cui B. 2021. The structural and functional properties of corn starch treated with endogenous malt amylases. Food Hydrocolloids, 117, 106722
Gujral H S, Rosell C M. 2004. Functionality of rice flour modified with a microbial transglutaminase. Journal of Cereal Science, 39, 225–230.
Guo J, Zhang Y, Yang X. 2012. A novel enzyme cross-linked gelation method for preparing food globular protein-based transparent hydrogel. Food Hydrocolloids, 26, 277–285.
Guo L, Zhu Y, Li J H, Gui Y F, Tao H T, Zou F X, Liu P F, Janaswamy S, Cui B. 2021. The effects of wheat amylose ratios on the structural and physicochemical properties of waxy rice starch using branching enzyme and glucoamylase. Food Hydrocolloids, 113, 106410.
Hamer R J. 2005. Coeliac disease: Background and biochemical aspects. Biotechnology Advances, 23, 401–408.
Hammes G G. 2007. Principles of chemical kinetics. Methods of Biochemical Analysis, 50, 75–96.
Isaschar-Ovdat S, Fishman A. 2017. Mechanistic insights into tyrosinase-mediated crosslinking of soy glycinin derived peptides. Food Chemistry, 232, 587–594.
Isaschar-Ovdat S, Fishman A. 2018. Crosslinking of food proteins mediated by oxidative enzymes - A review. Trends in Food Science & Technology, 72, 134–143.
Lakemond C M M, van Vliet V. 2008. Rheological properties of acid skim milk gels as affected by the spatial distribution of the structural elements and the interaction forces between them. International Dairy Journal, 18, 585–593.
Li B, Du W, Jin J, Du Q. 2012. Preservation of (–)-epigallocatechin-3-gallate antioxidant properties loaded in heat treated β-lactoglobulin nanoparticles. Journal of Agricultural and Food Chemistry, 60, 3477–3484.
Li J H, Zou F X, Gui Y F, Guo L, Wang N, Liu P F, Cui B. 2021. Long-term retrogradation properties of rice starch modified with transglucosidase. Food Hydrocolloids, 121,1070532.
Mattinen M, Lantto R, Selinheimo E, Kruus K, Buchert J. 2008. Oxidation of peptides and proteins by Trichoderma reesei and Agaricus bisporus tyrosinases. Journal of Biotechnology, 133, 395–402.
Mu T, Sun H. 2017. Progress in research and development of potato staple food processing technology. Journal of Applied Glycoscience, 64, 51–64.
Na Y L, Fen C S, Huang Y, Bo L, Wen Z Z, Xiao J Y. 2012. Thermal degradation kinetics of poly-alpha-methylstyrene. High Power Laser Part Beams, 24, 2400–2405.
Nickerson M T, Paulson A T, Speers R A. 2003. Rheological properties of gellan solutions: Effect of calcium ions and temperature on pre-gel formation. Food Hydrocolloids, 17, 577–583.
Nishinari K, Zhang H, Ikeda S. 2000. Hydrocolloid gels of polysaccharides and proteins. Current Opinion in Colloid & Interface Science, 5, 195–201.
Ribotta P D, Rosell C M. 2010. Effects of enzymatic modification of soybean protein on the pasting and rheological profile of starch–protein systems. Starch/Stärke, 62, 373–383.
Rosane M D, Soares F F, Scremin V S. 2005. Thermal stability of biodegradable films based on soy protein and corn starch. Macromolecular Symposia, 229, 258–265.
Schmidt J M, Damgaard H, Greve-Poulsen M, Sunds A V, Larsen L B, Hammershøj M. 2019. Gel properties of potato protein and the isolated fractions of patatins and protease inhibitors - Impact of drying method, protein concentration, pH and ionic strength. Food Hydrocolloids, 96, 246–258.
Shen L Q, Wang X Y, Wang Z Y, Wu Y F, Chen J S. 2008. Studies on tea protein extraction using alkaline and enzyme methods. Food Chemistry, 107, 929–938.
Surewicz W K, Mantsch H H. 1988. Solution and membrane structure of enkephalins as studied by infrared spectroscopy. Biochemical and Biophysical Research Communications, 150, 245–251.
Villanueva M, Harasym J, Muñoz J M, Ronda F. 2019. Rice flour physically modified by microwave radiation improves viscoelastic behavior of doughs and its bread-making performance. Food Hydrocolloids, 90, 472–481.
Wu X M, Liu Y W, Liu A J, Wang W H. 2017. Improved thermal-stability and mechanical properties of type I collagen by crosslinking with casein, keratin and soy protein isolate using transglutaminase. International Journal of Biological Macromolecules, 98, 292–301.
Xu X, Liu W, Zhong J, Luo L, Liu C, Luo S, Chen L. 2015. Binding interaction between rice glutelin and amylose: Hydrophobic interaction and conformational changes. International Journal of Biological Macromolecules, 81, 942–950.
Yuan C, Sang L Y, Wang Y L, Cui B. 2018. Influence of cyclodextrins on the gel properties of kappa-carrageenan. Food Chemistry, 266, 545–550.
Zhou D, Zhang B, Chen B, Chen H. 2017. Effects of oligosaccharides on pasting, thermal and rheological properties of sweet potato starch. Food Chemistry, 230, 516–523.
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