Analysis of genetic diversity and population structure in sweetpotato using SSR markers

doi:10.1016/j.jia.2023.02.004

Journal of Integrative Agriculture

2023, Vol. 22

Issue (11): 3408-3415 DOI: 10.1016/j.jia.2023.02.004

Crop Science

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Analysis of genetic diversity and population structure in sweetpotato using SSR markers

LIU Cheng*, ZHAO Ning*, JIANG Zhi-cheng, ZHANG Huan, ZHAI Hong, HE Shao-zhen, GAO Shao-pei, LIU Qing-chang^#

Key Laboratory of Sweetpotato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education/College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, P.R.China

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摘要

甘薯是世界上重要的粮食作物。大规模评价甘薯种质资源的遗传多样性，对明确甘薯种质资源间的遗传关系、有效利用这些种质资源对甘薯进行遗传改良是非常重要的。本研究使用30对多态性好的SSR引物，分析了617份甘薯种质资源的遗传多样性。这些甘薯种质资源包括中国的376个地方品种和162个育成品种以及来自其他11个国家的79个引进品种。根据群体结构分析，将这些甘薯种质资源分为3个群，即：群体1、群体2、群体3，它们分别含有228份、136份和253份种质资源。通过聚类分析和主成分分析（PCoA）也获得了一致的结果。在这3个群中，群体2显示出最高的遗传多样性水平，该群主要分布在低纬度地区。中国南方的甘薯种质资源具有最高的遗传多样性水平，这一结果支持甘薯最早由福建和广东传入中国的假说。分子方差分析（AMOVA）结果显示，不同群间存在显著的遗传分化，但是不同来源地和不同种质类型之间的遗传分化水平较低。这些结果为在甘薯育种中更好地利用这些种质资源提供了有价值的信息。

Abstract Sweetpotato, Ipomoea batatas (L.) Lam., is an important food crop worldwide. Large scale evaluation of sweetpotato germplasm for genetic diversity is necessary to determine the genetic relationships between them and effectively use them in the genetic improvement. In this study, the genetic diversity of 617 sweetpotato accessions, including 376 landraces and 162 bred varieties from China and 79 introduced varieties from 11 other countries, was assessed using 30 simple sequence repeat (SSR) primer pairs with high polymorphism. Based on the population structure analysis, these sweetpotato accessions were divided into three groups, Group 1, Group 2 and Group 3, which included 228, 136 and 253 accessions, respectively. Consistent results were obtained by phylogenic analysis and principal coordinate analysis (PCoA). Of the three groups, Group 2 showed the highest level of genetic diversity and its accessions were mainly distributed in low-latitude regions. The accessions from South China exhibited the highest level of genetic diversity, which supports the hypothesis that Fujian and Guangdong were the first regions where sweetpotato was introduced to China. Analysis of molecular variance (AMOVA) indicated significant genetic differentiations between the different groups, but low levels of genetic differentiation existed between the different origins and accession types. These results provide valuable information for the better utilization of these accessions in sweetpotato breeding.

Keywords: sweetpotato genetic diversity population structure SSR

Received: 11 October 2022 Accepted: 14 November 2022

Fund: This work was supported by the National Key R&D Program of China (2019YFD1001301 and 2019YFD1001300), the earmarked fund for CARS-10-Sweetpotato and the Hebei Key R&D Program, China (20326320D).

About author: #Correspondence LIU Qing-chang, Tel: +86-10-62733710, E-mail: liuqc@cau.edu.cn * These authors contributed equally to this study.

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	LIU Cheng
	ZHAO Ning
	JIANG Zhi-cheng
	ZHANG Huan
	ZHAI Hong
	HE Shao-zhen
	GAO Shao-pei
	LIU Qing-chang

Cite this article:

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