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Journal of Integrative Agriculture  2021, Vol. 20 Issue (11): 2914-2931    DOI: 10.1016/S2095-3119(20)63393-7
Special Issue: 园艺-分子生物合辑Horticulture — Genetics · Breeding
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Transcriptome analysis reveals effects of red and blue lightemitting diodes (LEDs) on the growth, chlorophyll fluorescence and endogenous plant hormones of potato (Solanum tuberosum L.) plantlets cultured in vitro
CHEN Li-li1, WANG Hao-ying2, GONG Xiao-chen2, ZENG Zhao-hai2, XUE Xu-zhang3, HU Yue-gao2
1 College of Science & Technology, Ningbo University, Cixi 315300, P.R.China
2 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
3 Beijing Research Center for Intelligent Equipment in Agriculture, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, P.R.China
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研究报道红、蓝光源显著影响植物幼苗生长。马铃薯是世界上重要的粮饲兼用作物。马铃薯组培苗培养在马铃薯生产中扮演重要角色。然而,从转录组水平上揭示红、蓝光源对马铃薯组培苗生长的影响研究较少。本研究的目的是借助转录组技术探索单色红光(RR)、单色蓝光(BB)和红蓝组合(RB)光谱处理的马铃薯组培苗的生长和生理反应。与对照RB相比,RR和BB处理的马铃薯组培苗分别被检测到有3150和814个差异表达基因。与对照相比,富集在“光合作用”和“光合天线蛋白”代谢通路上的差异表达基因分别被BB和RR处理所上调和下调表达,这可能与在上述两个处理中分别增加和降低的叶绿素荧光参数Fv/Fm, φPSⅡ,qp和ETR有关。BB处理的马铃薯组培苗呈现出茎秆矮化、叶片较大,而RR处理则表现为茎秆伸长,叶片较小。这些显著的形态改变与马铃薯组培苗叶和茎器官中内源激素GAs,IAA 和CKs含量不同有关。此外,单色红、蓝LEDs光源引起“植物激素信号传导”通路上差异基因相反的表达模式与其激素含量不同有密切关系。本研究在转录组水平上揭示了马铃薯组培苗对红、蓝LEDs光源的不同响应,并从光谱特征方面有助于马铃薯组培苗快繁。

Abstract  Red and blue light illumination has been reported to significantly affect plantlet growth.  Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production.  However, few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level.  The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red (RR), monochromatic blue (BB) as well as combined red and blue (RB) LEDs using the RNA-Seq technique.  In total, 3 150 and 814 differentially expressed genes (DEGs) were detected in potato plantlets under RR and BB, respectively, compared to RB (used as control).  Compared to the control, the DEGs enriched in “photosynthesis” and “photosynthesis-antenna proteins” metabolic pathways were up-regulated and down-regulated by BB and RR, respectively, which might be responsible for the increases and decreases of maximum quantum yield (Fv/Fm), photochemical quantum yield (φPSII), photochemical quenching (qP) and electron transfer rate (ETR) in BB and RR, respectively.  Potato plantlets exhibited dwarfed stems and extended leaves under BB, whereas elongated stems and small leaves were induced under RR.  These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin (GAs), indoleacetic acid (IAA) and cytokinins (CKs), as assessed in stems and leaves of potato plantlets.  In addition, monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the “plant hormone signal transduction” metabolic pathway, which were closely related to the endogenous plant hormone levels in potato plantlets.  Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect.
Keywords:  potato (Solanum tuberosum L.) plantlets in vitro        red/blue LEDs light sources        RNA-seq, chlorophyll fluorescence        plant hormone  
Received: 05 June 2020   Accepted:
Fund: This work was funded by the Scientific Research Fund of College of Science & Technology, Ningbo University for Introduction of High-level Talents, China (RC190006).
Corresponding Authors:  Correspondence HU Yue-gao, E-mail:; XUE Xu-zhang, E-mail:   
About author:  CHEN Li-li, E-mail:;

Cite this article: 

CHEN Li-li, WANG Hao-ying, GONG Xiao-chen, ZENG Zhao-hai, XUE Xu-zhang, HU Yue-gao. 2021. Transcriptome analysis reveals effects of red and blue lightemitting diodes (LEDs) on the growth, chlorophyll fluorescence and endogenous plant hormones of potato (Solanum tuberosum L.) plantlets cultured in vitro. Journal of Integrative Agriculture, 20(11): 2914-2931.

Aksenova N P, Konstantinova T N, Sergeeva L I, Machachkova I, Golyanoversuskaya S A. 1994. Morphogenesis of potato plants in vitro. I. effects of light quality and hormones. Journal of Plant Growth Regulation, 13, 143–146.
Baker N R. 2008. Chlorophyll fluorescence: A probe of photosynthesis in vivo. Annual Review of Plant Biology, 59, 89–113.
Ballare C L, Pierik R. 2017. The shade-avoidance syndrome: Multiple signals and ecological consequences. Plant Cell and Environment, 40, 2530–2543.
Chang S X, Li C X, Yao X Y, Chen S, Jiao X L, Liu X Y, Xu Z G. 2016. Morphological, photosynthetic, and physiological responses of rapeseed leaf to different combinations of red and blue lights at the rosette stage. Frontiers in Plant Science, 7, 1144.
Chen L L, Xue X Z, Yang Y D, Chen F, Zhao J, Wang X Q, Khan A T. 2018. Effects of red and blue LEDs on in vitro growth and microtuberization of potato single-node cuttings. Frontiers of Agricultural Science and Engineering, 5, 197–205.
Chen L L, Yang Y D, Jiang Y, Zhao J, Zang H D, Wang X F, Hu Y G, Xue X Z. 2019. RNA-Seq analysis reveals differential responses of potato (Solanum tuberosum L.) plantlets cultured in vitro to red, blue, green, and white light-emitting diodes (LEDs). Journal of Plant Growth Regulation, 38, 1412–1427.
Chen L L, Zhang K, Gong X C, Wang H Y, Gao Y H, Wang X Q, Zeng Z H, Hu Y G. 2020. Effects of different LEDs light spectrum on the growth, leaf anatomy and chloroplast ultrastructure of potato plants in vitro and minituber production after transplanting in the greenhouse. Journal of Integrative Agriculture, 19, 108–119.
Chory J, Reinecke D, Sim S, Washburn T, Brenner M. 1994. A role for cytokinins in de-etiolation in Arabidopsis - det mutants have an altered response to cytokinins. Plant Physiology, 104, 339–347.
Daviere J M, de Lucas M, Prat S. 2008. Transcriptional factor interaction: A central step in DELLA function. Current Opinion in Genetics and Development, 18, 295–303.
Demotes-Mainard S, Peron T, Corot A, Bertheloot J, Le-Gourrierec J, Pelleschi-Travier S, Crespel L, Morel P, Huche-Thelier L, Boumaza R, Vian A, Guerin V, Leduc N, Sakr S. 2016. Plant responses to red and far-red lights, applications in horticulture. Environmental and Experimental Botany, 121, 4–21.
Edesi J, Kotkas K, Pirttilä A M, Häggman H. 2014. Does light spectral quality affect survival and regeneration of potato (Solanum tuberosum L.) shoot tips after cryopreservation? Plant Cell Tissue and Organ Culture, 119, 599–607.
Evans J R, Morgan P B, von Caemmerer S. 2017. Light quality affects chloroplast electron transport rates estimated from chl fluorescence measurements. Plant and Cell Physiology, 58, 1652–1660.
Fukuda N, Ajima C, Yukawa T, Olsen J E. 2016. Antagonistic action of blue and red light on shoot elongation in petunia depends on gibberellin, but the effects on flowering are not generally linked to gibberellin. Environmental and Experimental Botany, 121, 102–111.
Gao J, Shi J G, Dong S T, Liu P, Zhao B, Zhang J W. 2018. Grain development and endogenous hormones in summer maize (Zea mays L.) submitted to different light conditions. International Journal of Biometeorology, 62, 2131–2138.
Gautam P, Terfa M T, Olsen J E, Torre S. 2015. Red and blue light effects on morphology and flowering of Petunia×hybrida. Scientia Horticulturae, 184, 171–178.
Huché-Thélier L, Crespel L, Gourrierec J L, Morel P, Sakr S, Leduc N. 2016. Light signaling and plant responses to blue and UV radiations - Perspectives for applications in horticulture. Environmental and Experimantal Botany, 121, 22–38.
Hwang I, Sheen J. 2001. Two-component circuitry in Arabidopsis cytokinin signal transduction. Nature, 413, 383–389.
Jungandreas A, Costa B S, Jakob T, Bergen M, Baumann S, Wilhelm C. 2014. The acclimation of Phaeodactylum tricornutum to blue and red light does not influence the photosynthetic light reaction but strongly disturbs the carbon allocation pattern. PLoS ONE, 9, e99727.
Kurepin L V, Emery R J, Pharis R P, Reid D M. 2007. Uncoupling light quality from light irradiance effects in Helianthus annuus shoots: Putative roles for plant hormones in leaf and internode growth. Journal of Experimental Botany, 58, 2145–2157.
Kurepin L V, Farrow S, Walton L J, Emery R J N, Pharis R P, Chinnappa C C. 2012. Phenotypic plasticity of sun and shade ecotypes of Stellaria longipes in response to light quality signaling: Cytokinins. Environmental and Experimental Botany, 84, 25–32.
Kurepin L V, Pharis R P, Emery R J N, Reid D M, Chinnappa C C. 2015. Phenotypic plasticity of sun and shade ecotypes of Stellaria longipes in response to light quality signaling, gibberellins and auxin. Plant Physiology and Biochemistry, 94, 174–180.
Kurepin L V, Walton L J, Pharis R P, Emery R J N, Reid D M. 2010. Interactions of temperature and light quality on phytohormone-mediated elongation of Helianthus annuus hypocotyls. Plant Growth Regulation, 64, 147–154.
Kurepin L V, Walton L J, Yeung E C, Reid D M. 2011. The interaction of light irradiance with auxin in regulating growth of Helianthus annuus shoots. Plant Growth Regulation, 65, 255–262.
Lee J S. 2006. Response to red and blue lights by electrical currents on the surface of intact leaves. Journal of Plant Biology, 49, 186–192.
Li C X, Xu Z G, Dong R Q, Chang S X, Wang L Z, Rehman K U M, Tao J M. 2017. An RNA-Seq analysis of grape plantlets grown in vitro reveals different responses to blue, green, red LED light, and white fluorescent light. Frontiers in Plant Science, 8, 78.
Ma X F, Wang Y P, Liu M X, Xu J M, Xu Z G. 2015. Effects of green and red lights on the growth and morphogenesis of potato (Solanum tuberosum L.) plantlets in vitro. Scientia Horticulturae, 190, 104–109.
Mao X, Cai T, Olyarchuk J G, Wei L. 2005. Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary. Bioinformatics, 21, 3787–3793.
Matysiak B, Gabryszewska E. 2016. The effect of in vitro culture conditions on the pattern of maximum photochemical efficiency of photosystem II during acclimatisation of Helleborus niger plantlets to ex vitro conditions. Plant Cell Tissue and Organ Culture, 125, 585–593.
Mishra S, Khurana J P. 2017. Emerging roles and new paradigms in signaling mechanisms of plant cryptochromes. Critical Reviews in Plant Sciences, 36, 89–115.
Murashige T, Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497.
Ouyang F, Mao J F, Wang J H, Zhang S G, Li Y. 2015. Transcriptome analysis reveals that red and blue light regulate growth and phytohormone metabolism in Norway spruce [Picea abies (L.) Karst]. PLoS ONE, 10, e0127896.
Pashkovskiy P P, Soshinkova T N, Korolkova D V, Kartashov A V, Zlobin I E, Lyubimov V Y, Kreslavski V D, Kuznetsov W. 2018. The effect of light quality on the pro-/antioxidant balance, activity of photosystem II, and expression of light-dependent genes in Eutrema salsugineum callus cells. Photosynthesis Research, 136, 199–214.
Roro A G, Dukker S A F, Melby T I, Solhaug K A, Torre S, Olsen J E. 2017. UV-B-induced inhibition of stem elongation and leaf expansion in pea depends on modulation of gibberellin metabolism and intact gibberellin signalling. Journal of Plant Growth Regulation, 36, 680–690.
Rosen H. 1957. A modified ninhydrin colorimetric analysis for amino acids. Archives of Biochemistry and Biophysics, 67, 10–15.
Roumeliotis E, Kloosterman B, Oortwijn M, Lange T, Visser R G, Bachem C W. 2013. Down regulation of StGA3ox genes in potato results in altered GA content and affect plant and tuber growth characteristics. Journal of Plant Physiology, 170, 1228–1234.
Schaller G E, Bishopp A, Kieber J J. 2015. The yin–yang of hormones: Cytokinin and auxin interactions in plant development. Plant Cell, 27, 44–63.
Schefe J H, Lehmann K E, Buschmann I R, Unger T, Funke-Kaiser H. 2006. Quantitative real-time RT-PCR data analysis: Current concepts and the novel “gene expression’s CT difference” formula. Journal of Molecular Medicine, 84, 901–910.
Seabrook J E A. 2005. Light effects on the growth and morphogenesis of potato (Solanum tuberosum) in vitro a review. American Journal of Potato Research, 82, 353–367.
Sergeeva L I, Machackova I, Konstantinova T N, Golyanovskaya S A, Josef E, Zaltsmanz O O, Hanu J, Aksenova N P. 1994. Morphogenesis of potato plants in vitro. II. endogenous levels, distribution, and metabolism of IAA and cytokinins. Journal of Plant Growth Regulation, 13, 147–152.
Su N N, Wu Q, Shen Z G, Xia K, Cui J. 2014. Effects of light quality on the chloroplastic ultrastructure and photosynthetic characteristics of cucumber seedlings. Plant Growth Regulation, 73, 227–235.
Tsai Y C, Weir N R, Hill K, Zhang W J, Kim H J, Shiu S H, Schaller G E, Kieber J J. 2012. Characterization of genes involved in cytokinin signaling and metabolism from rice. Plant Physiology, 158, 1666–1684.
Vinterhalter D, Vinterhalter B, Orbovic V. 2012. Photo- and gravitropic bending of potato plantlets obtained in vitro from single node explants. Journal of Plant Growth Regulation, 31, 560–569.
Warpeha K M, Montgomery B L. 2016. Light and hormone interactions in the seed-to-seedling transition. Environmental and Experimental Botany, 121, 56–65.
Weiler E, Jourdan P, Conrad W. 1981. Levels of indole-3-acetic in intact and decapitated coleoptiles as determined by a specific and highly sensitive solid-phase enzyme immunoassay. Planta, 153, 561–571.
Wilson D A, Weigel R C, Wheeler R M, Sager J C. 1993. Light spectral quality effects on the growth of potato (Solanum tuberosum L.) nodal cutting in vitro. In Vitro Cellular & Developmental Biology - Plant, 29P, 5–8.
Wu Y Y, Xing D K. 2013. Sterile measurement on the characteristics of chlorophyll fluorescence in plantlets in vitro preserved under low temperature condition. In: Proceedings of the 2013 3rd International Conference on Photonics and Image in Agriculture Engineering, 8762,  doi: 10.1117/12.2020252.
Yang F, Fan Y F, Wu X L, Cheng Y J, Liu Q L, Feng L Y, Chen J X, Wang Z L, Wang X C, Yong T W. 2018. Auxin-to-gibberellin ratio as a signal for light intensity and quality in regulating soybean growth and matter partitioning. Frontiers in Plant Science, 9, 56.
Yao X Y, Liu X Y, Xu Z G, Jiao X L. 2017. Effects of light intensity on leaf microstructure and growth of rape seedlings cultivated under a combination of red and blue LEDs. Journal of Integrative Agriculture, 16, 97–105.
Young M D, Wakefield M J, Smyth G K, Oshlack A. 2010. Gene ontology analysis for RNA-seq: Accounting for selection bias. Genome Biology, 11, R14.
Yu D Q, Qanmber G, Lu L L, Wang L L, Li J, Yang Z E, Liu Z, Li Y, Chen Q J, Mendu V. 2018. Genome-wide analysis of cotton GH3 subfamily II reveals functional divergence in fiber development, hormone response and plant architecture. BMC Plant Biology, 18, 350.
Yu W W, Liu Y, Song L L, Jacobs D F, Du X H, Ying Y Q, Shao Q S, Wu J S. 2016. Effect of differential light quality on morphology, photosynthesis, and antioxidant enzyme activity in camptotheca acuminata seedlings. Journal of Plant Growth Regulation, 36, 148–160.
Zheng L, Van Labeke M C. 2017a. Chrysanthemum morphology, photosynthetic efficiency and antioxidant capacity are differentially modified by light quality. Journal of Plant Physiology, 213, 66–74.
Zheng L, Van Labeke M C. 2017b. Long-term effects of red- and blue-light emitting diodes on leaf anatomy and photosynthetic efficiency of three ornamental pot plants. Frontiers in Plant Science, 8, 917.
Zhou Y H, Guo D P, Zhu Z J, Qian Q Q. 2005. Effects of in vitro rooting environments and irradiance on growth and photosynthesis of strawberry plantlets during acclimatization. Plant Cell Tissue and Organ Culture, 81, 105–108.
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