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Journal of Integrative Agriculture  2022, Vol. 21 Issue (10): 2865-2875    DOI: 10.1016/j.jia.2022.07.054
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Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD

LI Chen1, 2*, LIU Xuan-xuan2*, ABOUELNASR Hesham1, 3, MOHAMED HAMED Arisha1, 4, KOU Meng1, TANG Wei1, 2, YAN Hui1, 2, WANG Xin1, WANG Xiao-xiao2, ZHANG Yun-gang1, LIU Ya-ju1, GAO Run-fei1, MA Meng1, LI Qiang1, 2

1 Xuzhou Institute of Agricultural Sciences in Jiangsu Xuhuai District/Sweetpotato Research Institute, Chinese Academy of Agricultural Sciences/Key Laboratory of Biology and Genetic Breeding of Sweetpotato, Ministry of Agriculture and Rural Affairs, Xuzhou 221131, P.R.China
2 School of Life Science, Jiangsu Normal University, Xuzhou 221116, P.R.China
3 Plant Pathology Department, Agriculture and Biology research division, National research center, Giza 12622, Egypt
4 Department of Horticulture, Faculty of Agriculture, Zagazig University, Zagazig, Sharkia 44511, Egypt
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摘要  甘薯(Ipomoea batatas (L.) Lam.)作为一种重要的杂粮作物,在世界各地广泛种植,但深受甘薯病毒病(SPVD)的影响。本研究利用短串联靶标模拟物(STTM)成功抑制甘薯miR397的表达,上调其靶基因漆酶(IbLACs)的表达,使木质素合成途径的上游基因,包括苯丙氨酸解氨酶(PAL)、4-香豆酸辅酶A连接酶(4CL)、羟基肉桂酰CoA:莽草酸/奎宁酸羟基肉桂酰转移酶(HTC)、咖啡酸O-甲基转移酶(COMT)、肉桂醇脱氢酶(CAD)等基因被反馈调控而广泛上调表达。同时,导致PAL和LAC的酶活性显著增加,促进木质素的合成与积累。木质素在细胞壁中的沉积增加了转基因甘薯植株的物理防御能力,有效减少了烟粉虱对SPVD的传播,保证甘薯的健康生长。本研究为甘薯抗病育种和绿色生产提供了新思路。


As a critical food crop, sweetpotato (Ipomoea batatas (L.) Lam.) is widely planted all over the world, but it is deeply affected by Sweetpotato Virus Disease (SPVD).  The present study utilized short tandem target mimic (STTM) technology to effectively up-regulate the expression of laccase (IbLACs) by successfully inhibiting the expression of miR397.  The upstream genes in the lignin synthesis pathway were widely up-regulated by feedback regulation, including phenylalanine ammonialyase (PAL), 4-coumarate-CoAligase (4CL), hydroxycinnamoyl CoA:shikimatetransferase (HTC), caffeicacid O-methyltransferase (COMT), and cinnamyl alcohol dehydrogenase (CAD).  Meanwhile, the activities of PAL and LAC increased significantly, finally leading to increased lignin content.  Lignin deposition in the cell wall increased the physical defence ability of transgenic sweetpotato plants, reduced the accumulation of SPVD transmitted by Bemisia tabaci (Gennadius), and promoted healthy sweetpotato growth.  The results provide new insights for disease resistance breeding and green production of sweetpotato. 

Keywords:  sweetpotato        miR397        STTM        SPVD        lignin content  
Received: 22 March 2021   Accepted: 07 June 2021
Fund: This work was financially supported by the National Key R&D Program of China (2019YFD1001300 and 2019YFD1001305), the earmarked fund for CARS-10-Sweetpotato, and the Jiangsu Postgraduate Scientific Research and Practical Innovation Program Project, China (KYCX19-2207).

About author:  LI Chen, E-mail:; LIU Xuan-xuan, E-mail:; Correspondence LI Qiang, Tel: +86-516-82189203, Fax: +86-516-82189209, E-mail: * These authors contributed equally to this study.

Cite this article: 

LI Chen, LIU Xuan-xuan, ABOUELNASR Hesham, MOHAMED HAMED Arisha, KOU Meng, TANG Wei, YAN Hui, WANG Xin, WANG Xiao-xiao, ZHANG Yun-gang, LIU Ya-ju, GAO Run-fei, MA Meng, LI Qiang. 2022. Inhibition of miR397 by STTM technology to increase sweetpotato resistance to SPVD. Journal of Integrative Agriculture, 21(10): 2865-2875.

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