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Journal of Integrative Agriculture  2021, Vol. 20 Issue (1): 178-190    DOI: 10.1016/S2095-3119(20)63349-4
Special Issue: 昆虫合辑Plant Protection—Entomolgy
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Performance and transcriptomic response of the English grain aphid, Sitobion avenae, feeding on resistant and susceptible wheat cultivars
LAN Hao*, ZHANG Zhan-feng*, WU Jun, CAO He-he, LIU Tong-xian
Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling 712100, P.R.China
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植物抗虫性主要依靠限制害虫营养和有毒有害物质,但是,营养和有毒物质在植物抗蚜虫中的相对重要性仍未确定。在本论文中,我们探究了抗性小麦品种小偃22和易感小麦品种西农979对麦长管蚜的生长发育、繁殖、营养摄入量和转录组影响。结果显示,虽然小偃22的韧皮部汁液的营养质量(氨基酸:糖)比西农979的更高,但是,在小偃22上饲养的麦长管蚜的体重和繁殖力均显著低于在西农979上饲养的麦长管蚜。并且,在小偃22上饲养的麦长管蚜的蜜露分泌量也显著低于在西农979上饲养的麦长管蚜,这表明麦长管蚜在小偃22上饲养时,其摄入更少的韧皮部汁液。另外,通过比较在西农979和小偃22上饲养的麦长管蚜的转录组数据,我们发现共有600个差异表达基因,前20条差异表达基因显著富集的KEGG通路中有11条是与营养代谢相关的。我们共找到81个与糖、脂以及氨基酸代谢相关的差异表达基因,其中有59个差异表达基因是在小偃22上饲养的麦长管蚜中显著下调。另外,我们只找到18个与解毒代谢相关的差异表达基因,分别为8个UDP-glucuronosyltransferases,6个cytochromes P450 monooxygenases,1个glutathione S-transferase,2个ATP-binding cassette transporters以及1个major facilitator superfamily transporter,其中有12个差异表达基因是在小偃22上饲养的蚜虫中显著上调。以上结果说明,麦长管蚜从寄主小麦韧皮部获得的营养的数量和质量都对其生长发育很重要;小偃22较高的抗蚜性主要源于限制麦长管蚜取食韧皮部汁液,而非单纯依靠有毒物质。

Plant resistance against insects mainly depends on nutrient restriction and toxic metabolites, but the relative importance of nutrition and toxins remains elusive.  We examined performance, nutrition ingestion, and transcriptome response of the English grain aphid, Sitobion avenae, feeding on resistant Xiaoyan 22 (XY22) and susceptible Xinong 979 (XN979) wheat cultivars.  Aphids had lower body weight and fecundity when feeding on XY22 than on XN979, although the phloem sap of XY22 had a higher nutritive quality (in terms of amino acid:sucrose ratio).  Aphids feeding on XY22 also had a lower honeydew excretion rate than those on XN979, suggesting that aphids ingested less phloem sap from XY22.  The transcriptome data showed 600 differentially expressed genes (DEGs), and 11 of the top 20 KEGG pathways significantly enriched in DEGs were involved in nutrient metabolism.  We found 81 DEGs associated with the metabolism of sugars, lipids, and amino acids, 59 of which were significantly downregulated in aphids feeding on XY22.  In contrast, there were 18 DEGs related to detoxifying metabolism, namely eight UDP-glucuronosyltransferases, six cytochromes P450 monooxygenases, one glutathione S-transferase, two ATP-binding cassette transporters, and one major facilitator superfamily transporter; 12 of these were upregulated in the aphids feeding on XY22.  Our results indicated that both the quantity and quality of phloem nutrition available to aphids are critical for the growth and development of aphids, and the higher resistance of XY22 is mainly due to the reduction in phloem sap ingested by aphids, rather than toxic metabolites.
Keywords:  plant–aphid interaction        transcriptome response        nutrition restriction  
Received: 04 February 2020   Accepted:
Fund: This work was supported by the National Natural Science Foundation of China (31272089). We are grateful for the assistance of all the members in the Key Laboratory of Northwest Loess Plateau Crop Pest Management of Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling, Shaanxi, China.
Corresponding Authors:  Correspondence CAO He-he, Mobile: +86-15829727715, E-mail:; LIU Tong-xian, Tel: +86-29-87092663, E-mail:    
About author:  LAN Hao, E-mail:; ZHANG Zhan-feng, E-mail:; * These authors contributed equally to this study.

Cite this article: 

LAN Hao, ZHANG Zhan-feng, WU Jun, CAO He-he, LIU Tong-xian. 2021. Performance and transcriptomic response of the English grain aphid, Sitobion avenae, feeding on resistant and susceptible wheat cultivars. Journal of Integrative Agriculture, 20(1): 178-190.

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