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Journal of Integrative Agriculture  2022, Vol. 21 Issue (7): 2134-2144    DOI: 10.1016/S2095-3119(21)63733-4
Special Issue: 农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe
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Interaction of soil microbial communities and phosphorus fractions under long-term fertilization in paddy soil 
Muhammad QASWAR1, 2, Waqas AHMED3, HUANG Jing1, 4, LIU Kai-lou5, ZHANG Lu1, HAN Tian-fu1, DU Jiang-xue1, Sehrish ALI1, Hafeez UR-RAHIM6, HUANG Qing-hai5, ZHANG Hui-min1, 4
1 National Engineering Laboratory for Improving Quality of Arable Land/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 Department of Environment, Faculty of Bioscience Engineering, Ghent University, Ghent 9000, Belgium
3 Guangdong Provincial Key Laboratory for Radionuclides Pollution Control and Resources, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, P.R.China
4 National Observation Station of Qiyang Agri-ecology System/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Qiyang 426182, P.R.China
5 National Engineering and Technology Research Center for Red Soil Improvement/Jiangxi Institute of Red Soil, Nanchang 331717, P.R.China
6 Key Laboratory of Industrial Ecology and Environmental Engineering, Ministry of Education/School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, P.R.China
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Understanding the impact of biological activities on the soil phosphorus (P) distribution under long-term fertilizer application can facilitate better soil P fertility management.  Therefore, the primary objectives of this study were to investigate the effect of long-term (since 1981) fertilizer application on the soil P fractions and microbial community and to evaluate correlations between the microbial community structure and P distribution.  The following treatments were implemented in a long-term field trial: no fertilization (CK), inorganic N and K (NK), inorganic P and K (PK), inorganic N, P and K (NPK) and manure+NPK (MNPK) fertilization.  The study showed that the soil pH, soil organic carbon and total and available N and P concentrations were considerably higher in the MNPK treatment than in the CK treatment.  The soil microbial biomass C, N and P concentrations were also significantly higher in the MNPK treatment than in the CK treatment.  Among fertilization treatments, the β-1,4-glucosidase, α-1,4-glucosidase, urease, acid phosphatase and phosphodiesterase activities were the highest in the MNPK treatment.  Compared to inorganic fertilization, the MNPK treatment increased the labile soil P fractions and decreased the residual soil P concentration.  Continuous fertilization significantly affected the soil microbial composition.  The total phospholipid fatty acid (PLFA) concentrations in the NK, PK, NPK and MNPK treatments were 23.3, 43.1, 48.7 and 87.7% higher, respectively, than in the CK treatment.  A significant correlation was observed between the microbial community and soil P fractions.  Moreover, the aggregated boosted tree (ABT) model showed that among the various soil biochemical properties, the total PLFA concentration was the factor that most influenced the active P pool, accounting for 35.4% of the relative influence of all soil biochemical properties examined.  These findings reveal that combined manure and inorganic fertilizer application is a better approach than applying inorganic fertilizer alone for sustaining long-term P fertility by mediating soil biological activity.

Keywords:   enzyme activities        long-term fertilization        microbial community        microbial biomass        phosphorus fraction  
Received: 05 March 2021   Accepted: 11 May 2021
Fund: This work was supported by the National Key Research and Development Program of China (2016YFD0300901), the Fundamental Research Funds for Central Non-profit Scientific Institutions of China (1610132019035, 1610132020023 and 1610132020024), and the Natural Science Foundation of Jiangxi Province, China (20192BAB203022).
About author:  Muhammad QASWAR, E-mail:; Correspondence ZHANG Hui-min, E-mail:

Cite this article: 

Muhammad QASWAR, Waqas AHMED, HUANG Jing, LIU Kai-lou, ZHANG Lu, HAN Tian-fu, DU Jiang-xue, Sehrish ALI, Hafeez UR-RAHIM, HUANG Qing-hai, ZHANG Hui-min. 2022. Interaction of soil microbial communities and phosphorus fractions under long-term fertilization in paddy soil . Journal of Integrative Agriculture, 21(7): 2134-2144.

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