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Journal of Integrative Agriculture  2021, Vol. 20 Issue (8): 2255-2264    DOI: 10.1016/S2095-3119(20)63331-7
Special Issue: 农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe
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Functional diversity of soil microbial communities in response to supplementing 50% of the mineral N fertilizer with organic fertilizer in an oat field
ZHANG Mei-jun, JIA Ju-qing, LU Hua, FENG Mei-chen, YANG Wu-de

College of Agriculture, Shanxi Agricultural University, Taigu 030801, P.R.China
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利用Biolog-Eco平板研究氮肥减半配施有机肥对燕麦田土壤微生物群落代谢和多样性的影响。试验由5个处理组成:CK,不施肥;U1,90 kg ha–1 N的尿素;U2,45 kg ha–1 N的尿素;U2OM1,45 kg ha–1 N的尿素配施90 kg ha–1 N的羊粪有机肥;U2OM2,45 kg ha–1 N的尿素配施45 kg ha–1 N的羊粪有机肥。每个处理重复3次。试验于2018年和2019年在山西省平鲁县进行。结果表明,两年氮肥减半配施有机肥均有利于提高燕麦田土壤微生物群落对氨基酸,胺类,糖类,酸酸类和聚合物类碳源的利用。且对土壤微生物群落丰富度,优势度和均匀度也有显著促进作用。对氨基酸、胺类和酸酸类的利用以及对土壤微生物群落均匀度的影响随有机肥量增加而增强。双标图分析表明胺类和氨基酸是土壤微生物群落利用的主要碳源。2年结果均显示总氮量达135 kg ha–1,即45 kg ha–1 N的尿素配施90 kg ha–1 N的羊粪有机肥,燕麦产量最高。氮肥减半配施合适量的有机肥显著提高土壤微生物群落功能多样性。胺类和氨基酸类碳源可以作为总碳源的代表用于未来燕麦田土壤微生物群落对碳源利用的研究中。


The effects of supplementing 50% of the mineral N fertilizer with organic fertilizer on the metabolism and diversity of soil microbial communities in an oat field were investigated using Biolog-Eco plates.  The experiment consisted of five treatments: no fertilizer (CK), mineral N fertilizer applied at 90 and 45 kg ha–1 N in the form of urea (U1 and U2, respectively), and U2 supplemented with organic fertilizer  in the form of sheep manure at 90 and 45 kg ha–1 N (U2OM1 and U2OM2, respectively).  Each treatment had three replications.  The experiment was conducted in 2018 and 2019 in Pinglu District, Shanxi Province, China.  The carbon source utilization by soil microbial communities, such as amino acids, amines, carbohydrates, carboxylic acids, and polymers, increased when 50% of the mineral N fertilizer was replaced with organic fertilizer in both years.  This result was accompanied by increased richness, dominance, and evenness of the microbial communities.  The utilization of amino acid, amine, and carboxylic acid carbon sources and community evenness were further improved when the organic fertilizer amount was doubled in both years.  Biplot analysis indicated that amines and amino acids were the most representative of the total carbon source utilization by the soil microbial communities in both years.  The highest oat yield was achieved at a total N application rate of 135 kg ha–1 in the treatment involving 45 kg ha–1 N in the form of urea and 90 kg ha–1 N in the form of sheep manure in both years.  It was concluded that the application of 50% of the conventional rate of mineral N fertilizer supplemented with an appropriate rate of organic fertilizer enhanced both the functional diversity of soil microbial communities and oat yield.  Amine and amino acid carbon sources may be used as a substitute for total carbon sources for assessing total carbon source utilization by soil microbial communities in oat fields in future studies.
Keywords:  organic fertilizer        Biolog-Eco        soil microbial community        carbon source utilization        diversity        oats  
Received: 13 March 2020   Accepted:
Fund: This study was supported by the National Natural Science Foundation of China (72073131), the Central Public-Interest Scientific Institution Basal Research Fund, China (2020JKY025) and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2016-AII).
Corresponding Authors:  Correspondence HU Chen-pei, Fax: +86-10-68783928, E-mail:   
About author:  Correspondence YANG Wu-de, Tel: +86-354-6288206, E-mail:

Cite this article: 

ZHANG Mei-jun, JIA Ju-qing, LU Hua, FENG Mei-chen, YANG Wu-de. 2021. Functional diversity of soil microbial communities in response to supplementing 50% of the mineral N fertilizer with organic fertilizer in an oat field. Journal of Integrative Agriculture, 20(8): 2255-2264.

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