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Journal of Integrative Agriculture  2021, Vol. 20 Issue (6): 1649-1659    DOI: 10.1016/S2095-3119(20)63290-7
Special Issue: 农业生态环境-土壤微生物合辑Agro-ecosystem & Environment—Soil microbe
Agro-ecosystem & Environment Advanced Online Publication | Current Issue | Archive | Adv Search |
Microbial community dynamics during composting of animal manures contaminated with arsenic, copper, and oxytetracycline
Ebrahim SHEHATA1, 2, CHENG Deng-miao3, MA Qian-qian1, LI Yan-li1, LIU Yuan-wang1, FENG Yao1, JI Zhen-yu1, LI Zhao-jun1 
1 Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs/China–New Zealand Joint Laboratory for Soil Molecular Ecology, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2 Department of Natural Resources and Agricultural Engineering, College of Agriculture, Damanhour University, Damanhour 22511, Egypt
3 Research Center for Eco-Environmental Engineering, Dongguan University of Technology, Dongguan 523808, P.R.China
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Abstract  Effects of the heavy metal copper (Cu), the metalloid arsenic (As), and the antibiotic oxytetracycline (OTC) on bacterial community structure and diversity during cow and pig manure composting were investigated.  Eight treatments were applied, four to each manure type, namely cow manure with: (1) no additives (control), (2) addition of heavy metal and metalloid, (3) addition of OTC and (4) addition of OTC with heavy metal and metalloid; and pig manure with: (5) no additives (control), (6) addition of heavy metal and metalloid, (7) addition of OTC and (8) addition of OTC with heavy metal and metalloid.  After 35 days of composting, according to the alpha diversity indices, the combination treatment (OTC with heavy metal and metalloid) in pig manure was less harmful to microbial diversity than the control or heavy metal and metalloid treatments.  In cow manure, the treatment with heavy metal and metalloid was the most harmful to the microbial community, followed by the combination and OTC treatments.  The OTC and combination treatments had negative effects on the relative abundance of microbes in cow manure composts.  The dominant phyla in both manure composts included Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria.  The microbial diversity relative abundance transformation was dependent on the composting time.  Redundancy analysis (RDA) revealed that environmental parameters had the most influence on the bacterial communities.  In conclusion, the composting process is the most sustainable technology for reducing heavy metal and metalloid impacts and antibiotic contamination in cow and pig manure.  The physicochemical property variations in the manures had a significant effect on the microbial community during the composting process.  This study provides an improved understanding of bacterial community composition and its changes during the composting process. 
Keywords:  composting        heavy metal and metalloid        oxytetracycline        microbial community       principle component analysis        redundancy analysis  
Received: 03 March 2020   Accepted:
Fund: The work was supported financially by the National Key Technology R&D Program of China (2018YFD0500206), the National Natural Science Foundation of China (31572209, 31772395 and 31972943), and the Foundation for Safety of Agricultural Products by Ministry of Agriculture and Rural Affairs, China (GJFP2019033).
Corresponding Authors:  Correspondence LI Zhao-jun, Tel: +86-10-82108657, E-mail:   

Cite this article: 

Ebrahim SHEHATA, CHENG Deng-miao, MA Qian-qian, LI Yan-li, LIU Yuan-wang, FENG Yao, JI Zhen-yu, LI Zhao-jun . 2021. Microbial community dynamics during composting of animal manures contaminated with arsenic, copper, and oxytetracycline. Journal of Integrative Agriculture, 20(6): 1649-1659.

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