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Journal of Integrative Agriculture  2021, Vol. 20 Issue (11): 2849-2861    DOI: 10.1016/S2095-3119(20)63412-8
Special Issue: Triticeae Crops Genetics · Breeding · Germplasm Resources
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments
LIU Hang1*, TANG Hua-ping1*, LUO Wei1, MU Yang1, JIANG Qian-tao1, LIU Ya-xi1, CHEN Guo-yue1, WANG Ji-rui1, ZHENG Zhi2, QI Peng-fei1, JIANG Yun-feng1, CUI Fa3, SONG Yin-ming4, YAN Gui-jun5, WEI Yu-ming1, LAN Xiu-jin1, ZHENG You-liang1, MA Jian
1 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
2 Agriculture and Food, CSIRO, St Lucia, Brisbane 4217, Australia
3 College of Agriculture, Ludong University, Yantai 264000, P.R.China
4 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100093, P.R.China
5 School of Plant Biology, The University of Western Australia, Perth WA 6009, Australia
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Uppermost-internode diameter (UID) is a key morphological trait associated with spike development and yield potential in wheat.  Our understanding of its genetic basis remains largely unknown.  Here, quantitative trait loci (QTLs) for UID with high-density genetic maps were identified in five wheat recombinant inbred line (RIL) populations.  In total, 25 QTLs for UID were detected in five RIL populations, and they were located on chromosomes 1A, 1D (3 QTL), 2B (2), 2D (3), 3B, 3D, 4A, 4B (3), 4D, 5A (5), 5B (2), 6B, and 7D.  Of them, five major and stable QTLs (QUid.sau-2CN-1D.1, QUid.sau-2SY-1D, QUid.sau-QZ-2D, QUid.sau-SC-3D, and QUid.sau-AS-4B) were identified from each of the five RIL populations in multiple environments.  QUid.sau-2CN-1D.1, QUid.sau-2SY-1D and QUid.sau-SC-3D are novel QTLs.  Kompetitive Allele Specific PCR (KASP) markers tightly linked to them were further investigated for developing near-isogenic lines (NILs) carrying the major loci.  Furthermore, candidate genes at these intervals harboring major and stable QTLs were predicted, and they were associated with plant development and water transportation in most cases.  Comparison of physical locations of the identified QTL on the ‘Chinese Spring’ reference genome showed that several QTLs including two major ones, QUid.sau-2CN-1D.1 and QUid.sau-2SY-1D, are likely allelic confirming their validity and effectiveness.  The significant relationships detected between UID and other agronomic traits and a proper UID were discussed.  Collectively, our results dissected the underlying genetic basis for UID in wheat and laid a foundation for further fine mapping and map-based cloning of these QTLs.
Keywords:  uppermost-internode diameter        wheat        yield potential        genetic basis        quantitative trait loci  
Received: 04 June 2020   Accepted: 17 September 2021
Fund: This work was supported by the projects from the National Natural Science Foundation of China (31971937 and 31970243), the Key Projects of Scientific and Technological Activities for Overseas Students of Sichuan Province, China  and the Applied Basic Research Programs of Science and Technology Department of Sichuan Province, China (2020YJ0140). 
Corresponding Authors:  Correspondence MA Jian, Tel: +86-28-86293115, Fax: +86-28-82650350, E-mail:   
About author:  * These authors contributed equally to this study.

Cite this article: 

LIU Hang, TANG Hua-ping, LUO Wei, MU Yang, JIANG Qian-tao, LIU Ya-xi, CHEN Guo-yue, WANG Ji-rui, ZHENG Zhi, QI Peng-fei, JIANG Yun-feng, CUI Fa, SONG Yin-ming, YAN Gui-jun, WEI Yuming, LAN Xiu-jin, ZHENG You-liang, MA Jian. 2021. Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments. Journal of Integrative Agriculture, 20(11): 2849-2861.

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