Please wait a minute...
Journal of Integrative Agriculture  2021, Vol. 20 Issue (11): 2849-2861    DOI: 10.1016/S2095-3119(20)63412-8
Special Issue: Triticeae Crops Genetics · Breeding · Germplasm Resources
Crop Science Advanced Online Publication | Current Issue | Archive | Adv Search |
Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments
LIU Hang1*, TANG Hua-ping1*, LUO Wei1, MU Yang1, JIANG Qian-tao1, LIU Ya-xi1, CHEN Guo-yue1, WANG Ji-rui1, ZHENG Zhi2, QI Peng-fei1, JIANG Yun-feng1, CUI Fa3, SONG Yin-ming4, YAN Gui-jun5, WEI Yu-ming1, LAN Xiu-jin1, ZHENG You-liang1, MA Jian
1 State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Ministry of Science and Technology/Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, P.R.China
2 Agriculture and Food, CSIRO, St Lucia, Brisbane 4217, Australia
3 College of Agriculture, Ludong University, Yantai 264000, P.R.China
4 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100093, P.R.China
5 School of Plant Biology, The University of Western Australia, Perth WA 6009, Australia
Download:  PDF in ScienceDirect  
Export:  BibTeX | EndNote (RIS)      
Abstract  
Uppermost-internode diameter (UID) is a key morphological trait associated with spike development and yield potential in wheat.  Our understanding of its genetic basis remains largely unknown.  Here, quantitative trait loci (QTLs) for UID with high-density genetic maps were identified in five wheat recombinant inbred line (RIL) populations.  In total, 25 QTLs for UID were detected in five RIL populations, and they were located on chromosomes 1A, 1D (3 QTL), 2B (2), 2D (3), 3B, 3D, 4A, 4B (3), 4D, 5A (5), 5B (2), 6B, and 7D.  Of them, five major and stable QTLs (QUid.sau-2CN-1D.1, QUid.sau-2SY-1D, QUid.sau-QZ-2D, QUid.sau-SC-3D, and QUid.sau-AS-4B) were identified from each of the five RIL populations in multiple environments.  QUid.sau-2CN-1D.1, QUid.sau-2SY-1D and QUid.sau-SC-3D are novel QTLs.  Kompetitive Allele Specific PCR (KASP) markers tightly linked to them were further investigated for developing near-isogenic lines (NILs) carrying the major loci.  Furthermore, candidate genes at these intervals harboring major and stable QTLs were predicted, and they were associated with plant development and water transportation in most cases.  Comparison of physical locations of the identified QTL on the ‘Chinese Spring’ reference genome showed that several QTLs including two major ones, QUid.sau-2CN-1D.1 and QUid.sau-2SY-1D, are likely allelic confirming their validity and effectiveness.  The significant relationships detected between UID and other agronomic traits and a proper UID were discussed.  Collectively, our results dissected the underlying genetic basis for UID in wheat and laid a foundation for further fine mapping and map-based cloning of these QTLs.
Keywords:  uppermost-internode diameter        wheat        yield potential        genetic basis        quantitative trait loci  
Received: 04 June 2020   Accepted: 17 September 2021
Fund: This work was supported by the projects from the National Natural Science Foundation of China (31971937 and 31970243), the Key Projects of Scientific and Technological Activities for Overseas Students of Sichuan Province, China  and the Applied Basic Research Programs of Science and Technology Department of Sichuan Province, China (2020YJ0140). 
Corresponding Authors:  Correspondence MA Jian, Tel: +86-28-86293115, Fax: +86-28-82650350, E-mail: jianma@sicau.edu.cn   
About author:  * These authors contributed equally to this study.

Cite this article: 

LIU Hang, TANG Hua-ping, LUO Wei, MU Yang, JIANG Qian-tao, LIU Ya-xi, CHEN Guo-yue, WANG Ji-rui, ZHENG Zhi, QI Peng-fei, JIANG Yun-feng, CUI Fa, SONG Yin-ming, YAN Gui-jun, WEI Yuming, LAN Xiu-jin, ZHENG You-liang, MA Jian. 2021. Genetic dissection of wheat uppermost-internode diameter and its association with agronomic traits in five recombinant inbred line populations at various field environments. Journal of Integrative Agriculture, 20(11): 2849-2861.

Avni R, Nave M, Barad O, Baruch K, Twardziok S O, Gundlach H, Hale I, Mascher M, Spannagl M, Wiebe K. 2017. Wild emmer genome architecture and diversity elucidate wheat evolution and domestication. Science, 357, 93–97.
Berry P M, Berry S T. 2015. Understanding the genetic control of lodging-associated plant characters in winter wheat (Triticum aestivum L.). Euphytica, 205, 1–19.
Cui F, Li J, Ding A M, Zhao C H, Wang L, Wang X Q, Li S S, Bao Y G, Li X F, Feng D S. 2011. Conditional QTL mapping for plant height with respect to the length of the spike and internode in two mapping populations of wheat. Theoretical and Applied Genetics, 122, 1517–1536.
Deng M, Wu F Q, Zhou W L, Li J, Shi H R, Wang Z Q, Lin Y, Yang X L, Wei Y M, Zheng Y L, Liu Y X. 2019. Mapping of QTL for total spikelet number per spike on chromosome 2D in wheat using a high-density genetic map. Genetics and Molecular Biology, 42, 603–610.
Goddard M E. 1992. A mixed model for analyses of data on multiple genetic markers.Theoretical and Applied Genetics, 83, 878–886.
Huang H. 1998. Relation between the tissue of the highest internode and the number of spikelets. Acta Agronomica Sinica, 24, 200–202. (in Chinese)
IWGSC (International Wheat Genome Sequencing Consortium). 2018. Shifting the limits in wheat research and breeding using a fully annotated reference genome by the international wheat genome sequencing consortium. Science, 361, eaar7191.
Jiang Y F, Lan X J, Luo W, Kong X C, Qi P F, Wang J R, Wei Y M, Jiang Q T, Liu Y X, Peng Y Y. 2014. Genome-wide quantitative trait locus mapping identifies multiple major loci for brittle rachis and threshability in Tibetan semi-wild wheat (Triticum aestivum ssp. tibetanum Shao). PLoS ONE, 9, e114066.
Kaldenhoff R, Ribascarbo M, Sans J F, Lovisolo C, Heckwolf M, Uehlein N. 2008. Aquaporins and plant water balance. Plant Cell and Environment, 31, 658–666.
Li C, Ma J, Liu H, Ding P Y, Yang C C, Zhang H, Qin N N, Lan X J. 2019. Detection of QTLs for spike length and plant height in wheat based on 55K SNP array. Journal of Triticeae Crops, 39, 4. (in Chinese)
Lin H, Guo H J, Xiao S H, Jiang G L, Zhang X Y, Yan C S, Xin Z Y, Jia J Z. 2005. Quantitative trait loci (QTL) of stem strength and related traits in a doubled-haploid population of wheat (Triticum aestivum L.). Euphytica, 141, 1–9.
Lin H, Qian H, Zhuang J, Lu J, Min S, Xiong Z, Huang N, Zheng K. 1996. RFLP mapping of QTLs for yield and related characters in rice (Oryza sativa L.). Theoretical and Applied Genetics, 92, 920–927.
Liu G L, Mei H W, Yu X Q, Zou G H, Liu H Y, Hu S P, Li M S, Wu J H, Chen L, Luo L J. 2008. QTL analysis of panicle neck diameter, a trait highly correlated with panicle size, under well-watered and drought conditions in rice (Oryza sativa L.). Plant Science, 174, 71–77.
Liu J J, Luo W, Qin N N, Ding P Y, Zhang H, Yang C C, Mu Y, Tang H P, Liu Y X, Li W, Jiang Q T, Chen G Y, Wei Y M, Zheng Y L, Liu C J, Lan X J, Ma J. 2018. A 55 K SNP array-based genetic map and its utilization in QTL mapping for productive tiller number in common wheat. Theoretical and Applied Genetics, 131, 2439–2450.
Liu J J, Tang H P, Qu X R, Liu H, Li C, Tu Y, Li S Q, Ahsan Habib, Mu Y, Dai S F, Deng M, Jiang Q T, Liu Y X, Chen G Y, Wang J R, Chen G D, Li W, Jiang Y F, Wei Y M, Lan X J, et al. 2020. A novel, major, and validated QTL for the effective tiller number located on chromosome arm 1BL in bread wheat. Plant Molecular Biology, 104, 173–185.
Liu K Y, Xu H, Liu G, Guan P F, Zhou X Y, Peng H R, Yao Y Y, Ni Z F, Sun Q X, Du J K. 2018. QTL mapping of flag leaf-related traits in wheat (Triticum aestivum L.). Theoretical and Applied Genetics, 131, 839–849.
Luo M C, Gu Y Q, Puiu D, Wang H, Twardziok S O, Deal K R, Huo N X, Zhu T T, Wang L, Wang Y. 2017. Genome sequence of the progenitor of the wheat D genome Aegilops tauschii. Nature, 551, 498.
Luo W, Ma J, Zhou X H, Jiang Y F, Sun M, Yang Y J, Kong X C, Qi P F, Jiang Q T, Liu Y X, Peng Y Y, Chen G Y, Wei Y M, Zheng Y L, Lan X J. 2016a. Genetic analysis of glume hairiness (Hg) gene in bread wheat (Triticum aestivum L.). Genetic Resources and Crop Evolution, 63, 763–769.
Luo W, Ma J, Zhou X H, Sun M, Kong X C, Wei Y M, Jiang Y F, Qi P F, Jiang Q T, Liu Y X, Peng Y Y, Chen G Y, Zheng Y L, Liu C J, Lan X J. 2016b. Identification of quantitative trait loci controlling agronomic traits indicates breeding potential of Tibetan semiwild wheat (Triticum aestivum ssp. tibetanum). Crop Science, 56, 2410–2420.
Ma J, Ding P Y, Liu J J, Li T, Zou Y Y, Habib A, Mu Y, Tang H P, Jiang Q T, Liu Y X, Chen G Y, Wang J R, Deng M, Qi P F, Li W, Pu Z E, Zheng Y L, Wei Y M, Lan X J. 2019a. Identification and validation of a major and stably expressed QTL for spikelet number per spike in bread wheat. Theoretical and Applied Genetics, 132, 3155–3167.
Ma J, Qin N N, Cai B, Chen G Y, Ding P Y, Zhang H, Yang C C, Huang L, Mu Y, Tang H P, Liu Y X, Wang J R, Qi P F, Jiang Q T, Zheng Y L, Liu C J, Lan X J, Wei Y. 2019b. Identification and validation of a novel major QTL for all-stage stripe rust resistance on 1BL in the winter wheat line 20828. Theoretical and Applied Genetics, 132, 1363–1373.
Ma J, Tu Y, Zhu J, Luo W, Liu H, Li C, Li S Q, Liu J J, Ding P Y, Ahsan H, Mu Y, Tang H P, Liu C J, Jiang Q T, Chen G D, Wang J R, Li W, Pu Z E, Zheng Y L, Wei Y M, et al. 2019c. Flag leaf size and posture of bread wheat: genetic dissection, QTL validation and their relationships with yield-related traits. Theoretical and Applied Genetics, 133, 297–315.
Ma J, Yan G J, Liu C J. 2012. Development of near-isogenic lines for a major QTL on 3BL conferring Fusarium crown rot resistance in hexaploid wheat. Euphytica, 183, 147–152.
Morita M T, Sakaguchi K, Kiyose S I, Taira K, Kato T, Nakamura M, Tasaka M. 2006. A C2H2-type zinc finger protein, SGR5, is involved in early events of gravitropism in Arabidopsis inflorescence stems. The Plant Journal, 47, 619–628.
Pan X Y, Miao F. 2005. Survey of wheat microtubule tissue structure. Chinese Agricultural Science Bulletin, 9, 121–123. (in Chinese)
Piepho H P, Möhring J, Melchinger A E, Büchse A. 2008. BLUP for phenotypic selection in plant breeding and variety testing. Euphytica, 161, 209–228.
Qiao Y L, Piao R H, Shi J X, Lee S I, Jiang W Z, Kim B K, Lee J, Han L Z, Ma W B, Koh H J. 2011. Fine mapping and candidate gene analysis of dense and erect panicle 3, DEP3, which confers high grain yield in rice (Oryza sativa L.). Theoretical and Applied Genetics, 122, 1439–1449.
Qiu Z F, Fang C, Chen H J. 1987. On the vascular tissue of wheat internodal and its relationship to the grain number per spike. Acta Agronomica Sinica, 2, 102–102. (in Chinese)
Rahaie M, Xue G P, Naghavi M R, Alizadeh H, Schenk P M. 2010. A MYB gene from wheat (Triticum aestivum L.) is up-regulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes. Plant Cell Reports, 29, 835–844.
Ratcliffe O J, Riechmann J L. 2002. Arabidopsis transcription factors and the regulation of flowering time: A genomic perspective. Current Issues in Molecular Biology, 4, 77–92.
Sallam A, Hashad M, Hamed E S, Omara M. 2015. Genetic variation of stem characters in wheat and their relation to kernel weight under drought and heat stresses. Journal of Crop Science and Biotechnology, 18, 137–146.
Sang Y, Zhao L, Zhang K P, Tian J C, Ye B X. 2010. Mapping QTLs for uppermost internode diameter and thickness and area of culm wall with doubled-haploid population in wheat. Acta Agronomica Sinica, 36, 61–67. (in Chinese)
Shen H B, Yang D L, Jing R L, Chang X P, Qu Y Y. 2007. Genetic characters of vascular bundle in the first internode and its relationship with yield components of wheat. Journal of Triticeae Crops, 27, 465–470. (in Chinese)
Simonneau T, Habib R, Goutouly J P, Huguet J G. 1993. Diurnal changes in stem diameter depend upon variations in water content: Direct evidence in peach trees. Journal of Experimental Botan, 44, 615–621.
Smith S E, Kuehl R O, Ray I M, Hui R, Soleri D. 1998. Evaluation of simple methods for estimating broad-sense heritability in stands of randomly planted genotypes. Crop Science, 38, 1125–1129.
Tian J C, Deng Z Y, Zhang K P, Yu H X, Jiang X L, Li C. 2015. Genetic analysis of main physiological and morphological traits. In: Genetic Analyses of Wheat and Molecular Marker-Assisted Breeding. vol. 1. Springer. pp. 351–443.
Wiarda S L. 2015. Effect of the tiller inhibition (tin) genein winter wheat. Ph D thesis, Purdue University, USA.
Xie Q G. 2011. QTL mapping of lodging related traits in wheat. MSc thesis, Shandong Agricultural University, China. (in Chinese)
Xu X W. 2019. QTL mapping of wheat growth and development and stem related traits. MSc thesis, Northwest A&F University, China. (in Chinese)
Yang J. 2016. QTL mapping for pre-harvest sprouting resistance and molecular characterization of six grain germination-related genes in synthetic wheat. Ph D thesis, Sichuan Agricultural University, China. (in Chinese)
Yu M, Chen G Y, Pu Z E, Zhang L Q, Liu D C, Lan X J, Wei Y M, Zheng Y L. 2015. Quantitative trait locus mapping for growth duration and its timing components in wheat. Molecular Breeding, 35, 44.
Zhang D Q. 2017. QTL mapping for stalk related traits in wheat. MSc thesis, Northwest A&F University. (in Chinese)
Zhang H J, Li T, Liu H W, Mai C Y, Yu G J, Li H L, Yu L Q, Meng L Z, Jian D W, Yang L, Li H J, Zhou Y. 2020. Genetic progress in stem lodging resistance of the dominant wheat cultivars adapted to Yellow-Huai River Valleys Winter Wheat Zone in China since 1964. Journal of Integrative Agriculture, 19, 438–448.
Zhang L Q, Liu D C, Yan Z H, Lan X J, Zheng Y L, Zhou Y H. 2004. Rapid changes of microsatellite flanking sequence in the allopolyploidization of new synthesized hexaploid wheat. Science China (Life Sciences), 47, 553–561.
Zhou C Y, Xiong H C, Li Y T, Guo H J, Xie Y D, Zhao L S, Gu J Y, Zhao S R, Ding Y P, Song X Y, Liu L X. 2021. Genetic analysis and QTL mapping of a novel reduced height gene in common wheat (Triticum aestivum L.). Journal of Integrative Agriculture, 19, 1721–1730.
[1] ZHAO Lai-bin, XIE Die, HUANG Lei, ZHANG Shu-jie, LUO Jiang-tao, JIANG Bo, NING Shun-zong, ZHANG Lian-quan, YUAN Zhong-wei, WANG Ji-rui, ZHENG You-liang, LIU Deng-cai, HAO Ming. Integrating the physical and genetic map of bread wheat facilitates the detection of chromosomal rearrangements[J]. >Journal of Integrative Agriculture, 2021, 20(9): 2333-2342.
[2] LI Si-nan, CHEN Wen, MA Xin-yao, TIAN Xia-xia, LIU Yao, HUANG Li-li, KANG Zhen-sheng, ZHAO Jie. Identification of eight Berberis species from the Yunnan-Guizhou plateau as aecial hosts for Puccinia striiformis f. sp. tritici, the wheat stripe rust pathogen[J]. >Journal of Integrative Agriculture, 2021, 20(6): 1563-1569.
[3] LIU Yang, LI Yu-xiang, LI Yi-xiang, TIAN Zhong-wei, HU Jin-ling, Steve ADKINS, DAI Ting-bo. Changes of oxidative metabolism in the roots of wheat (Triticum aestivum L.) seedlings in response to elevated ammonium concentrations[J]. >Journal of Integrative Agriculture, 2021, 20(5): 1216-1228.
[4] BAI Sheng-sheng, ZHANG Han-bing, HAN Jing, WU Jian-hui, LI Jia-chuang, GENG Xing-xia, LÜ Bo-ya, XIE Song-feng, HAN De-jun, ZHAO Ji-xin, YANG Qun-hui, WU Jun, CHEN Xin-hong . Identification of genetic locus with resistance to take-all in the wheat-Psathyrostachys huashanica Keng introgression line H148[J]. >Journal of Integrative Agriculture, 2021, 20(12): 3101-3113.
[5] LIU Da-zhong, YANG Fei-fei, LIU Sheng-ping. Estimating wheat fractional vegetation cover using a density peak k-means algorithm based on hyperspectral image data[J]. >Journal of Integrative Agriculture, 2021, 20(11): 2880-2891.
[6] XIAO Jing-xiu, ZHU Ying-an, BAI Wen-lian, LIU Zhen-yang, TANG Li, ZHENG Yi. Yield performance and optimal nitrogen and phosphorus application rates in wheat and faba bean intercropping[J]. >Journal of Integrative Agriculture, 2021, 20(11): 3012-3025.
[7] LIU Rui-xuan, WU Fang-kun, YI Xin, LIN Yu, WANG Zhi-qiang, LIU Shi-hang, DENG Mei, MA Jian, WEI Yu-ming, ZHENG You-liang, LIU Ya-xi. Quantitative trait loci analysis for root traits in synthetic hexaploid wheat under drought stress conditions[J]. >Journal of Integrative Agriculture, 2020, 19(8): 1947-1960.
[8] DING Xiao-yu, XU Jin-song, HUANG He, QIAO Xing, SHEN Ming-zhen, CHENG Yong, ZHANG Xue-kun. Unraveling waterlogging tolerance-related traits with QTL analysis in reciprocal intervarietal introgression lines using genotyping by sequencing in rapeseed (Brassica napus L.)[J]. >Journal of Integrative Agriculture, 2020, 19(8): 1974-1983.
[9] PAN Li-jun, LU Lin, LIU Yu-ping, WEN Sheng-xian, ZHANG Zeng-yan. The M43 domain-containing metalloprotease RcMEP1 in Rhizoctonia cerealis is a pathogenicity factor during the fungus infection to wheat[J]. >Journal of Integrative Agriculture, 2020, 19(8): 2044-2055.
[10] Tahmina SHAR, SHENG Zhong-hua, Umed ALI, Sajid FIAZ, WEI Xiang-jin, XIE Li-hong, JIAO Gui-ai, Fahad ALI, SHAO Gao-neng, HU Shi-kai, HU Pei-song, TANG Shao-qing. Mapping quantitative trait loci associated with starch paste viscosity attributes by using double haploid populations of rice (Oryza sativa L.)[J]. >Journal of Integrative Agriculture, 2020, 19(7): 1691-1703.
[11] ZHOU Chun-yun, XIONG Hong-chun, LI Yu-ting, GUO Hui-jun, XIE Yong-dun, ZHAO Lin-shu, GU Jiayu, ZHAO Shi-rong, DING Yu-ping, SONG Xi-yun, LIU Lu-xiang. Genetic analysis and QTL mapping of a novel reduced height gene in common wheat (Triticum aestivum L.)[J]. >Journal of Integrative Agriculture, 2020, 19(7): 1721-1730.
[12] FANG Zheng-wu, HE Yi-qin, LIU Yi-ke, JIANG Wen-qiang, SONG Jing-han, WANG Shu-ping, MA Dong-fang, YIN Jun-liang. Bioinformatic identification and analyses of the non-specific lipid transfer proteins in wheat[J]. >Journal of Integrative Agriculture, 2020, 19(5): 1170-1185.
[13] LIANG Pan-pan, ZHAO Chen, LIN Yuan, GENG Ji-jia, CHEN Yuan, CHEN De-hua, ZHANG Xiang. Effects of sodium benzoate on growth and physiological characteristics of wheat seedlings under compound heavy metal stress[J]. >Journal of Integrative Agriculture, 2020, 19(4): 1010-1018.
[14] YUAN Wan-ling, XU Bo, RAN Gang-chao, CHEN Hui-ping, ZHAO Peng-yue, HUANG Qi-liang. Application of imidacloprid controlled-release granules to enhance the utilization rate and control wheat aphid on winter wheat[J]. >Journal of Integrative Agriculture, 2020, 19(12): 3045-3053.
[15] Seth TOLLEY, Yang Yang, Mohsen MOHAMMADI. High-throughput phenotyping identifies plant growth differences under well-watered and drought treatments[J]. >Journal of Integrative Agriculture, 2020, 19(10): 2429-2438.
No Suggested Reading articles found!